The relationship between nutrients and phytoplankton community structure in northern Beibu Gulf

根据2011年4月和8月北部湾北部(北部湾20°n以北的海域)的调查数据,北部湾北部春夏两季共鉴定出浮游植物312种,隶属于4门78属,硅藻是主要优势类群。春季优势种为细弱海链藻(THAlASSIOSIrA SubTIlIS)、丹麦细柱藻(lEPTOCylIndruS dAnICuS)、奇异棍形藻(bACIllArIA PArAdOXA)和尖刺拟菱形藻(PSEudOnITzSCHIA PungEnS);夏季优势种仅为海链藻(THAlASSIOSIrA SP.)。浮游植物群落在春季可划分为2个子群落(SPr1和SPr2),在夏季可划分为3个子群落(SuM1、SuM2和SuM3)。该海域n/P比值一直处于高的水平,绝大部分的水域表现为磷限制,这可能是硅藻大量生长所造成的。春季SPr2群落优势种的柔弱角毛藻(CHAETOCErOS dEbIlIS)与除铵盐之外的营养盐具显著相关关系,奇异棍形藻与环境因子无相关关系。夏季SuM3群落优势种的奇异棍形藻与磷酸盐具显著相关关系。分析表明,琼州海峡过道水为北部湾北部输送了磷酸盐,而硅酸盐则主要由陆地径流输入。Phytoplankton community and nutrient concentration in the northern Beibu Gulf in April and August 2011 were investigated.In this research, 312 phytoplankton taxa were identified, which belonged to four phyla and 78 genera, with Bacillariophyta being the main group.The dominant species were Thalassiosira subtilis, Leptocylindrus danicus, Bacillaria paradoxa, and Pseudonitzschia pungens in spring, and Thalassiosira sp.in summer.Phytoplankton community in northern Beibu Gulf could be divided into two natural groups in spring(spr1 & spr2) and three in summer(sum1, sum2 & sum3).N/P ratio was in a relatively high level in the study area, and most of the waters were phosphorus limitated which resulted from diatom blossom.Chaetoceros debilis was the dominant species of group spr1 and had significant correlation with nutrient in spring, except for ammonium.Bacillaria paradoxa was the dominant species of group sum3 and had significant correlation with phosphate in summer, but, it was uncorrelated with any environmental factors as the dominant species of group spr2 in spring.Phosphate was transported to northern Beibu Gulf through the Qiongzhou Strait corridor water, and silicate was input by land runoff.海洋公益性行业科研专项项目(201005012

miR-92b-3p靶向肌细胞增强子2D抑制心肌细胞肥大

【目的】探讨微小RNA microRNA-92b-3p（miR-92b-3p）对心肌细胞肥大的调控作用及其作用靶基因。【方法】建 立血管紧张素Ⅱ（Ang-Ⅱ）诱导的C57BL/6小鼠心肌肥厚模型。通过尾静脉注射胆固醇修饰的miR-92b-3p模拟物（agomiR- 92b-3p）来增加小鼠心肌中miR-92b-3p水平，分别设立3个实验组：agomiR-negative control （agomiR-NC）+saline组，agomiR- NC+Ang-Ⅱ组，agomiR-92b-3p+Ang-Ⅱ组。用Ang-Ⅱ诱导乳小鼠心肌细胞建立心肌细胞肥大模型；双荧光素酶报告基因实验 检测miR-92b-3p与潜在靶基因MEF2D 3'端非翻译区（3'-UTR）的结合作用；蛋白印迹法检测MEF2D及肥厚相关蛋白的表 达。【结果】（1）Ang-II诱导的小鼠肥厚心肌和肥大心肌细胞分别与对照组中心肌肥厚相关基因ANP、ACTA1和β-MHC水平 比较，差异具有统计学意义（P<0.05）；（2）双荧光素酶报告基因实验结果提示miR-92b-3p与MEF2D 3′UTR具有相互结合作 用；miR-92b-3p可在转录后水平抑制MEF2D的表达；升高miR-92b-3p或降低MEF2D水平能一致性地抑制Ang-II诱导的乳 小鼠心肌细胞肥大表型。【结论】MEF2D是miR-92b-3p的靶基因，并介导了miR-92b-3p发挥抑制心肌细胞肥大的作用

一种二维近程微波全息成像方法

本发明公开一种二维近程微波全息成像方法，其通过真实测量或模拟方式获取目标散射场和入射场的参数数据，能够获取更为真实的目标散射场和入射场，从而解决了近程毫米波全息成像技术中假设入射场为球面波或柱面波的不合理性；求解目标图像中利用超定方程组的最小二乘解作为目标函数的二维傅里叶变换，能够扩大成像目标的范围以及提高目标在不同的背景环境下的成像的清晰度和精准度