EFFECT OF MONOSODIUM GLUTAMATE ON ALUMINUM RETENTION IN TISSUES AND CAPACITY OF ANTIOXIDATION IN RATS

目的 :　了解谷氨酸钠对铝在组织中沉积及其对体内抗氧化能力的影响。方法 :　采用 2 4只 SD大鼠 ,体重约 ( 1 2 5± 1 5 ) g,平均分成 4组 ,进行为期 5 0 d的灌服试验 ,分别灌服蒸馏水 (空白对照组 ) ,1 .2 mmol氯化铝 ,1 .2 mmol柠檬酸钠 + 1 .2 mmol氯化铝 ,1 .2 mmol谷氨酸钠+ 1 .2 mmol氯化铝。取脑、肝、肾、骨组织及血液 ,测定组织中铝的含量 ,并测定血浆 MDA量和SOD活力。结果 :　给试验鼠服用铝 ,都能提高铝的吸收量 ,使各组织中铝的含量升高。同时给予铝和柠檬酸盐时 ,试验鼠肾、骨中铝的含量极显著地高于对照组和 Al Cl3 组 ( P<0 .0 1 ) ,肝中铝含量极显著地高于对照组 ( P<0 .0 1 ) ,脑中铝含量也显著高于对照组 ( P<0 .0 5 )。同时给予铝和谷氨酸钠 ,试验鼠肾、脑中铝的含量极显著地高于对照组和 Al Cl3 组 ( P<0 .0 1 ) ,血红细胞中铝的含量显著地高于对照组 ( P<0 .0 5 )。试验鼠同时服用铝和柠檬酸钠或谷氨酸钠将减弱血浆的抗氧化能力 ,血浆中 MDA的量明显增加 ,SOD酶活力则显著降低 ( P<0 .0 1 )。结论 :　谷氨酸钠和柠檬酸钠均能促进铝在组织中的沉积 ,减弱血浆的抗氧化能力。谷氨酸钠很可能是其α- NH2 ,α- CH-及 - COO-与Al形成络合物的结果。Objective: To investigate the effect of sodium glutamate on aluminum retention in tissues and capacity of antioxidation in rats. Methods: Twenty four SD rats were divided into 4 groups and gavaged with 1 ml of following solutions: distilled water, 1.2 mmol AlCl 3, 1.2 mmol AlCl 3+1.2 mmol sodium citrate (Al+Cit Na), and 1.2 mmol AlCl 3+1.2 mmol sodium glutamate (Al+Glu Na) for 50 days respectively. At the end of experiment, the tibia, liver, kidney, brain and blood were collected. Aluminum content of these tissues and red blood cells, malondialdehyde and superoxide dismutase of the plasma were determined. Results: The animals gavaged with aluminum had high aluminum content in the tissues. Al+Cit Na increased the aluminum level very significantly in the kidney and tibia compared to control and AlCl 3 (P<0.01) and very significantly(P<0.01)in liver and signicantly (P<0.05)in brain compared to control. Al+Glu Na elevated the aluminum level very significantly (P<0.05) in kidney and brain compared to control and AlCl 3 (P<0.01), significantly (P<0.05) in red blood cells compared to control. Absorption of aluminum increased malondialdehyde and decreased the activity of plasma superoxide dismutase very significantly. Conclusion: Monosodium glutamate and sodium citrate improve the aluminum retention in tissues and decrease plasma antioxidant capacity. A chelate complex is possibly formed from the α NH 2, α CH , and COO of glutamate and Al.厦门大学现代分析科学教育部重点实验室开放课题的资