Study of effects of diabetes on oocyte maturation and islet transplantation therapy

目的：Ⅰ型糖尿病孕妇在怀孕期间面临严重的生殖问题，如不育、流产、胎儿先天发育异常等等。越来越多证据表明糖尿病对胚胎的这种影响可能是由于母体的高糖环境影响到卵母细胞成熟造成的。胰岛移植是否可以逆转糖尿病对胚胎和卵母细胞成熟造成的影响、能否通过辅助干细胞治疗的方法降低胰岛移植中使用的免疫抑制剂对胚胎和卵母细胞成熟造成的影响等一系列问题需要进行系统的研究。为了解决这些问题，本文首先系统研究糖尿病对胚胎和卵母细胞成熟造成的影响；其次创新性的对比研究了胰岛素注射和胰岛移植逆转糖尿病对胚胎和卵母细胞成熟造成的影响的作用；最后，针对胰岛移植中免疫抑制剂对卵母细胞和胚胎的副作用，研究iPS诱导的MSCs（in...Objective: Women with poorly controlled type 1diabetes often suffer from a series of reproductive problems such as miscarriage, neonatal morbidity and mortality, and congenital malformations. Maternal hyperglycemia has been shown to adversely af-fect progression from a one-cell to a blastocyst stage in rodent models. Emerging evidence has implicated that these effects are associated with compromis...学位：理学博士院系专业：医学院_生理学学号：2452010015407

Advantages of double-embedding with agar and paraffin in making light microscope slices of small vessels

预先将固定好的小血管包埋到1.5%琼脂中,调整好其位置和管腔。采用自制的琼脂包埋模子可以方便快捷的获得结构完整大小适中的组织琼脂块。再按照常规的的脱水包埋程序做石蜡包埋,最后切片染色。结果显示,琼脂石蜡双包埋法保持了小血管管腔的完整性和规则性,内膜等组织结构几乎没有损坏和丢失,最终得到较为理想的光镜切片。The fixed small vessels were pre-embedded into 1.5% agar in advance,and its position and lumen were adjusted well.By using self-made agarembedding mould,the agar block in complete structure and right size could be obtained conveniently.Then paraffin embedding was done according to the conventional dehydration and embedding procedure,and staining was the last step.The results indicated that,by using the double embedding method,the integrity of lumen was kept,the intima and other tissues of vessel were not damaged and lost,the ideal light microscope slices were gotten ultimately.国家重点基础研究发展规划项目资助(973计划项目)(编号:2012CBA01303