Study on the Secondary Metabolites Produced by Two Specific Habitats Actinomycetes and One Marine Bacterium

随着陆地可培养微生物资源的大量开发和研究，从中发现新菌种、新代谢产物的可能性日趋减小。于是人们将目光投向了海洋和植物内共生菌等新资源的研究。这些特殊生境的微生物，能够产生结构新颖、种类繁多及活性独特的次级代谢产物，已成为发现新活性物质的重要来源。 本论文对一株海洋细菌、一株海洋放线菌和一株植物内生菌的次级代谢产物进行了研究，旨在发掘具有较高生物活性的新天然产物，为开发新药奠定基础。 对海洋细菌（Pseudomonassp.1896）进行了30L发酵罐发酵培养，并对其次生代谢产物进行了分离纯化，通过波谱学方法对化合物的结构进行了测定，共鉴定了5个环二肽化合物，分别为cyclo-(D-pro-...As the terrestrial sources have been fully researched, the possibility has become declined gradually that found new species and new secondary metabolites. Thus, people pay attention to the study of new groups from marine and plant endophytes. The microorganisms with special ecological environment can produce numerous metabolites with novel skeletons and varied and unique bioactivities. They have b...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2162007115196

Ansamycins Produced by Streptomyces sp. LZ35

目的对海洋放线菌STrEPTOMyCES SP.lz35固体发酵提取物的化学成分进行研究。方法采用SEPHAdEX lH-20,硅胶等色谱柱及重结晶进行分离纯化,用波谱技术鉴定化合物的结构。结果从14 l固体发酵提取物中分离得到7个安莎霉素类化合物,分别鉴定为gEldAnAMyCIn(1),17-O-dEMETHylgEldAnAMyCIn(2),HErbIMyCIn b(3),rEblASTATIn(4),17-O-dEMETHylrEblASTA-TIn(5),AuTOlyTIMyCIn(6),HygrOCIn A dErIVATIVE(7)。结论化合物1~7均为首次从该菌株中分离得到,且首次从一株链霉菌中分离到2种类型的安莎霉素。OBJECTIVE To study the chemical constituents of Streptomyces sp.LZ35.METHODS The constituents were isolated and purified by Sephadex LH-20 column,silicagel column and recrystallization,and their structures were identified by NMR and ESI-MS techniques.RESULTS Seven compounds were isolated and identified as geldanamycin(1),17-O-demethylgeldanamycin(2),herbimycin B(3),reblastatin(4),17-O-demethylreblastatin(5),autolytimycin(6),hygrocin A derivative(7),respectively.CONCLUSION Compounds 1-7 were obtained from the strain for the first time.Two types of ansamycins were isolated for the first time from Streptomyces sp.国家杰出青年科学基金(30325044);教育部科学技术研究重点(重大)项目(306010);山东大学自主创新基金(2010TB016;2010TS072

Relationship between genetic lineages and pathotypes of Magnaporthe grisea isolates from Fujian

采用rEP-PCr技术对福建省189个稻瘟病菌菌株进行dnA指纹图谱分析.结果表明:在58%相似水平上可以将供试菌株分为15个遗传谱系,表明该菌具有丰富的多态性.其中谱系fJl04出现频率为35.98%,为优势谱系.利用全国统一生理小种鉴别体系和lTH-nIlS鉴别体系从供试的189个稻瘟病菌株中分别鉴别出15和21个致病型(生理小种),优势型分别为zb13和J76.2,出现频率分别为58.72%和28.57%.采用这2套鉴别体系鉴别的致病型与dnA指纹图谱之间无明显的对应关系.The DNA fingerprints of 189 isolates of Magnaporthe grisea from Fujian were constructed using rep-PCR technique.All isolates could be divided into 15 genetic lineages at 58% similarity level.Among the 15 genetic lineages,FJL04 that consisted of 68 isolates was recognized as dominant lineages with occurrence frequency of 35.98%.The 189 isolates could be respectively classified into 15 and 21 pathotypes by Chinese race differentials and LTH-NILs,and their dominance types were ZB13 and J76.2 with occurrence frequency of 58.72% and 28.57%,respectively.There was no obviously corresponding relationship between pathotypes and genetic lineages.福建省科技厅资助项目(2005N030、2006N0034);福建省农科院青年科技人才创新基金资助项目(200708

Five Cyclodipeptides Isolated from Pseudomonas sp. 1896

通信作者: yshen1965@ gmail. com[中文文摘]以海洋细菌假单胞菌1896(Pseudomonas sp.1896)为研究对象,对其发酵液的化学成分进行研究.从30 L发酵液乙酸乙酯提取物中分离到5个化合物,并通过1D,2D-NMR和ESI-MS光谱数据确定了化合物的结构.它们分别为cyclo-(L-pro-L-tyr)(Ⅰ)、cyclo-(D-pro-L-tyr)(Ⅱ)、cyclo-(D-pro-L-val)(Ⅲ)、cyclo-(D-pro-D-trp)(Ⅳ)、cyclo-(L-pro-D-met)(Ⅴ).研究结果表明,海洋细菌中蕴藏着丰富的具有生物活性的环二肽,是开发微生物药物的重要资源.[英文文摘]To explore the chemical components from the fermentation of marine Pseudomonas sp.1896,five compounds were isolated and purified by column chromatography.The structures of compounds were elucidated by spectroscopic analysis,including 1D,2D-NMR data and mass-spectrometric analysis.They were identified as cyclo-(L-pro-L-tyr)(Ⅰ),cyclo-(D-pro-L-tyr)(Ⅱ),cyclo-(D-pro-L-val)(Ⅲ),cyclo-(D-pro-D-trp)(Ⅳ),cyclo-(L-pro-D-met)(Ⅴ).The results indicated that marine bacteria could be a promising source of searching leader.compo unds for drug discovery .国家高技术研究发展计划(863)项目(2007AA091904