8 research outputs found

    The Role of PI3K/Akt/HIF-1α Signaling Pathway in Bleomycin-Induced Pulmonary Fibrosis in Mice

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    目的探讨PI3K/Akt/HIF-1α信号通路在博来霉素(BLM)诱导的小鼠肺纤维化中的作用机制。方法 56只C57BL/6小鼠随机分为对照组和实验组,实验组通过气管内滴注BLM(2.5 mg/kg)建立肺纤维化模型,对照组在相同条件下气管内滴注等量0.9%氯化钠溶液。在造模第21 d取小鼠肺组织标本行HE和Masson染色分析肺组织形态学变化;运用Ashcroft评分以及检测羟脯氨酸含量评估肺纤维化程度;Western blot方法检测PI3K/Akt/HIF-1α信号通路的变化以及肺泡表面活性物质(Pro-SPC)蛋白含量;实时定量PCR(RT-PCR)法检测胶原蛋白3(Collagen3)mRNA表达水平;免疫组织化学法检测肺组织内Collagen3蛋白和细胞凋亡数的变化。结果实验组与对照组相比,肺泡炎和肺纤维化程度明显加重,肺组织内充填大量炎细胞及纤维病灶。试验组Ashcroft评分和羟脯氨酸含量均较对照组显著升高(P<0.05)。同时,PI3K/Akt/HIF-1α信号通路在实验组肺内明显活化,并伴有Pro-SPC蛋白生成减少,Collagen3蛋白含量及mRNA水平增加,以及肺内细胞凋亡数明显增加。结论 PI3K/Akt/HIF-1α信号通路的异常活化促进了肺纤维化形成。Objective To investigate the role of PI3K/Akt/HIF-1α signaling pathway in bleomycininduced pulmonary fibrosis in mice.Methods Fifty-six C57 BL/6 mice were randomly divided into a control group and a bleomycin(BLM)group.The pulmonary fibrosis model was induced by single intratracheal instillation of BLM(2.5 mg/kg)in the BLM group.Similarly,0.9% saline was instilled directly into the trachea in the control group.Then all mice were sacrificed on 21 stday.The lungs were collected for morphometric analysis with HE and Masson staining.The degree of pulmonary fibrosis was evaluated with Ashcroft score and content of hydroxyproline.The activity of PI3 K/Akt/HIF-1α signaling pathway and prosurfactant protein C(Pro-SPC)were measured by Western blot.The level of collagen3 mRNA was assessed with quantitative real time PCR analysis.Collagen3 protein and numbers of apoptosis cells were observed with immuno-histochemistry.Results It was exhibited that the thickening alveolar septa,accumulation of inflammatory cells,and fibrous obliteration in the BLM group but not in the control group.There was a significant difference in Ashcroft score and hydryoproline content in the BLM group.Meanwhile,the activity of PI3 K/Akt/HIF-1α signaling pathway was up-regulated and the protein of Pro-SPC was decreased in the BLM group.It was revealed that the numbers of apoptosis cells,expressions of Collagen3 protein and mRNA were increased in the BLM group.Conclusion Aberrant activity of PI3 K/Akt/HIF-1α signaling pathway may aggravate the pulmonary fibrogenesis.福建省科技局课题(编号:J00162

    关于不定方程组x~2-22y~2=1与y~2-Dz~2=1764的公解

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    利用Pell方程的解的性质及递归序列的方法,证明了不定方程组x2-22y2=1与y2-Dz2=1764有以下结果:当D=2p1…ps,1≤s≤4(p1,…,ps为互异的奇素数)时,此方程组的整数解为(i)D≠2×77617时,仅有平凡解x,y,(z)=±197,±42,(0);(ii)D=2×77617时,有非平凡解x,y,(z)=±30580901,±6519870,±(16548)和平凡解(x,y,z)=±197,±42,(0).当D=pm(m∈Z+,p为任意素数)时,其整数解只有平凡解(x,y,z)=±197,±42,(0).国家自然科学基金项目(61462016);;贵州省教育厅青年科技人才成长项目(黔教合KY字[2016]130

    半群OPP IE*(X)的秩

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    设X为有限全序集,E为X上的凸等价关系.令OPP IE*(X)为所有E类方向保序变换所构成的半群,得到了OPP IE*(X)的秩

    Research on wireless sensor network applied in highway transportation monitoring system

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    Recent advances in digital circuit, radio communication, and MEMS technologies, have made wireless sensor network (WSN) improve greatly. WSN can be applicated in many areas such as military areas, habitat monitoring, traffic monitoring, and so on. The other way, transportation is looked as one of the most important issues for the development of social economy, especially highway. In order to improve the management and efficiency of highway, and to prevent effectively and resolve traffic accidents in time, a highway transportation monitoring system based on WSN is put forward in this paper. This system adequately makes use of WSN&#39;s distribution, self-organizing and extensibility. The architecture and mechanism of the system are described. And some functions of the system have been implemented. The speed measurement of high-speed moving targets with multiple sensors has been accomplished, and the performance and limitation have been analyzed

    黄河三角洲生态区土壤石油污染及其与理化性质的关系      

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    基于黄河三角洲生态区内部分油田生产区石油污染土壤的调查取样,研究了土壤的石油污染特性及其与理化性质的关系。结果表明,油田区土壤受到了不同程度的石油污染,溢油处油泥含油率最高达6230mg/kg。石油污染土壤总有机碳(TOC)含量与含油率呈正相关关系,研究区TOC为0.22%~1.14%,其中老油井含量大于新油井。但值得注意的是本次研究发现部分石油污染区域土壤的TOC反而低于未污染区,这可能与碱蓬的覆盖状况有关。石油污染对土壤的全氮含量没有显著影响,所研究的石油生产区土壤总氮含量为207.74~870.20mg/kg,未受石油污染的生态核心区该值为591.02~938.28mg/kg。土壤有机碳、全氮及C/N随土壤深度的增加皆呈下降趋势,土壤各层中有机碳和全氮含量的变化趋势基本一致,呈极显著线性相关。石油烃污染导致石油污染土壤中C、N比例严重失调,因此在进行石油污染土壤修复时需要添加相应的N营养元素来增强土壤中微生物的营养来增强土壤中微生物的活性,加快其对石油的分解。

    手性及非中心对称配位聚合物的组装

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    微小孔径激光器的工艺及器件功率和寿命特性分析

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    在650 nm波长半导体激光器的基础上制造出了高输出功率的微小孔径激光器.驱动电流为25 mA时,输出功率达到0.4mW,最大功率可达1 mW以上.叙述了微小孔径激光器的特殊的制造工艺,并分析了器件可能的失效机理
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