北京生产性服务业与制造业的关联及空间分布

生产性服务业与制造业的有机融合、互动发展既是全球产业发展的趋势,也是中国走新型工业化道路的重要途径。以北京市为案例区,运用相关分析、投入产出模型、空间自相关模型、变异系数、地理联系率等,对生产性服务业与制造业互动的产业关联与空间分布进行实证研究,旨在为首都产业结构优化、空间布局调整提供科学依据。研究表明:①生产性服务业投入与制造业效益提升呈现正相关性,但制造业服务化程度较低;②制造业对生产性服务业的中间需求结构不断提升,不同类型制造业对生产性服务业的中间需求结构存在明显差异;③生产性服务业对资源密集型制造业的中间投入趋于下降,对技术密集型制造业的中间投入趋于上升,不同类型生产性服务业对制造业的中间投入结构存在明显差异;④制造业与配套生产性服务业均呈现显著的空间集聚性,但集聚与分散的空间格局存在明显差异,就业空间分布的一致性较差,进一步验证了制造业与配套生产性服务业具有空间可分性

北京城市低保家庭水价承受能力及用水状况研究

从兼顾效率与公平的角度出发,通过分析低保家庭用水承受能力,探讨了关注低保家庭生活用水的重要性和必要性,并对低保家庭的实际生活用水情况进行了研究.通过分析整理问卷调查获取的第一手数据,本文从用水角度定性评价了低保家庭生活状况和生活质量,并着重运用统计分析方法定量研究了北京城市低保家庭生活用水状况,建立了北京城市低保家庭生活用水计量模型,并提出了相关对策

Role of transcription factor YY1 in regulation of ITGB6 gene expression in oral squamous cell carcinoma

目的:研究肿瘤相关转录因子yy1对口腔鳞癌细胞整合素β6(InTEgrInβ6,ITgb6)基因表达调控的影响。方法:用生物信息学方法预测分布在ITgb6启动子区域的转录因子yy1潜在的结合位点,构建萤光素酶报告基因质粒,利用双萤光素酶报告基因系统检测ITgb6启动子片段的转录活性;利用染色质免疫沉淀技术检测在天然染色质条件下转录因子yy1与ITgb6启动子的结合情况;采用定点突变方法检测yy1结合位点对ITgb6启动子活性的影响;利用rT-PCr方法检测过表达转录因子yy1对口腔鳞癌细胞ITgb6 MrnA表达水平的影响。结果:ITgb6启动子-421~-150 nT区域存在多个转录因子yy1潜在的结合位点。在口腔鳞癌细胞的天然染色质中,转录因子yy1结合于ITgb6启动子-421~-150 nT区域;定点突变yy1潜在结合位点对口腔鳞癌细胞中ITgb6启动子活性无显著影响。另外,过表达的yy1对口腔鳞癌细胞ITgb6 MrnA的表达水平也无影响。结论:转录因子yy1在口腔鳞癌细胞中结合于ITgb6基因启动子-421~-150 nT区域,但对ITgb6基因的基础转录水平无影响。AIM: To explore the role of transcription factor YY1 in the regulation of integrin β6( ITGB6) gene expression in oral squamous cell carcinoma.METHODS: The distribution of the potential YY1 binding sites in ITGB6 promoter were predicted by bioinformatic methods.Series of 5' deletion of ITGB6 promoter luciferase reporter constructs containing potential YY1 binding sites were made and transfected into 293T cell line to detect the promoter activity.The binding activity of the transcription factor YY1 to ITGB6 promoter in the native chromatin environment was determined by chromatin immunoprecipitation( ChIP) assay.The role of the potential YY1 binding sites in the regulation of ITGB6 promoter activity was analyzed by substitution mutant analysis.The effect of YY1 over-expression on the mRNA expression of ITGB6 in oral squamous cell carcinoma cell line was measured by RT-PCR.RESULTS: Bioinformatics analysis revealed that there were several potential binding sites for YY1 in the region of-421 ~-150 nt in the ITGB6 promoter.ChIP assay showed that transcription factor YY1 bound to the ITGB6 promoter region of-421 ~-150 nt in the native chromatin environment.Substitution mutant analysis of potential YY1 binding sites in ITGB6 promoter did not affect the promoter activity of ITGB6.The over-expression of YY1 in oral squamous cell carcinoma cells did not affect the ITGB6 mRNA expression.CONCLUSION: The transcription factor YY1 binds to the region of-421 ~-150 nt in the ITGB6 promoter.However,it is not involved in the basic transcriptional regulation of ITGB6 gene in oral squamous cell carcinoma cells.国家自然科学基金资助项目(No.30900661;No.81072208;No.81370160); 教育部留学回国人员科研启动基金资助项目; 汕头大学医学院基础与临床科研基金资助项