Investigation on the in vitro inhibitory mechanisms of resveratrol on varicella-zoster virus

【目的】 1、应用构建的水痘-带状疱疹病毒（VZV）报告细胞系MV9G进一步研究白黎芦醇体外抑制VZV的作用机制。 2、进一步验证应用VZV报告细胞MV9G筛选抗VZV药物并研究其作用机制的可行性。 【方法】 1、将无细胞VZV直接感染MV9G细胞（CFVs直接感染）或将带细胞VZV与MV9G细胞共培养（CAVs共培养）以激发MV9G细胞表达报告基因萤火虫荧光素酶。 2、在CFVs直接感染前或CAVs共培养不同时间点加入白黎芦醇，通过比较药物对CFVs或CAVs激发荧光素酶的抑制强度分析白黎芦醇直接灭活病毒、抑制病毒粘附和穿透、抑制病毒在细胞内复制及其时间点和可逆性。 3、通过...Objectives 1. To further investigate the in vitro inhibitory mechanism(s) of resveratrol on varicella-zoster virus (VZV) with our previously generated reporter cell line MV9G. 2. To further verify the application of VZV reporter cell line MV9G to screening for anti-VZV drug and investigating its inhibitory mechanisms. Methods 1. Cell-free VZVs (CFVs) were directly inoculated onto monolayer...学位：医学硕士院系专业：医学院_肿瘤学学号：2452010115338

Anti-demodecidosis activity and skin safety ofJatropha curcas.1 leaves extract in vitro

目的 探讨麻风树叶治疗蠕形螨病的应用价值。方法 用80%乙醇热回流提取法提取麻风树叶提取液。取蠕形螨感染者面部皮脂,分离并鉴定蠕形螨备用。设不同浓度麻风树叶实验组、2%浓度的甲硝唑对照组和生理盐水对照组,进行体外抗螨实验。pH仪测定不同浓度麻风树叶提取物pH值。设麻风树叶实验组和75%乙醇对照组,用健康家兔进行皮肤刺激实验和急性皮肤毒性实验。结果 50、25、12 mg/ml麻风树叶组与2%甲硝唑组毛囊蠕形螨死亡时间分别为（1.55±0.67）min、（1.61±0.67）min、（2.47±0.80）min和（1.20±0.48）min。50、25 mg/ml麻风树叶组以及2%甲硝唑组两两之间差异无统计学意义（P均〉0.05）。50、25、12 mg/ml麻风树叶提取液pH值分别为6.07±0.73、6.27±0.82、6.35±0.83,对家兔完整皮肤及破损皮肤刺激评分均为0,且无明显毒性。结论 麻风树叶提取物具有较强的体外抗蠕形螨活性且具有皮肤安全性。Objective To test the application value of the extract of Jatropha curcas.l leaves in treatment of demodecidosis. Methods Jatropha curcas.1 leaves were extracted with 80% ethanol by using heat reflux method. Facial sebum specimen from demodecidosis-infected patients were used to isolate and identify demodecidosis. Different concentration groups of Jatropha curcas.l leaves, 2% metronidazole control group and physiological saline control group were defined. The anti-demodecidosis experiment was performed in vitro. The pH value of different concentrations of Jatropha curcas.l leaves extract was determined. Skin irritation test and acute skin toxicity test were carried out in healthy rabbits, and Jatropha curcas.1 leaves and 75% ethanol served as experiment and control groups, respectivelyl Results The mite-killing time was （1.55-4-0.67） min with 50 mg/ml Jatropha curcas.l leaves extract, （1.61-4-0.67） min with 25 mg/ml Jatropha curcas.l leaves extract, （2.47-4-0.80） min with 12 mg/ml Jatropha curcas.l leaves extract, and （1.20-4-0.48） min with 2% metronidazole. There was no significant different between 50, 25 mg/ml Jatropha curcas.l leaves extract groups and 2% metronidazole group （P 〉 0.05）. The pH value was 6.07＋0.73 of 50 mg/ml Jatropha curcas.l leaves extract, 6.27-1-0.82 of 25 mg/ml Jatropha curcas.l leaves extract and 6.35-4-0.83 of 12 mg/ml Jatropha curcas.1 leaves extract. Score for irritation to normal and wounded rabbit skin was both 0, and acute toxicity test showed no significant toxicity. Conclusions Jatropha curcas.l leaves extract shows a remarkable activity to demodecidosis with skin safety in vitro.漳州市自然科学基金（ZZ2014J33

Study on the morphologic change of Caofeidian islands

在解译1979-2012年间典型历史时期遥感影像的基础上,研究曹妃甸诸岛形态演变情况。研究显示,曹妃甸岛围填海（2003年）前自然变化较小,但整体形态呈逐渐缩小、向内陆弯曲趋势。随通岛路的建成（2005年）,自然岛体消失,人工岛体面积逐渐扩大。除曹妃甸因围填海面积扩大外,其周边诸岛均呈面积缩小趋势,已经处于末年期,自然演化状态已趋于终止。分析曹妃甸诸岛演变过程,除受围填海和挖沙等人为活动影响外,来水来沙量减少、风暴潮频发、水动力条件影响是曹妃甸诸岛演变的主要自然影响因素

近三十年来曹妃甸岸线岛体时空演变特征分析

在对1979年、1987年、1991年、1997年、2002年-2005年、2008年、2012年十个时期曹妃甸遥感影像图解译的基础上,从自然地理学角度对岸线和岛体历史演变趋势进行系统分析,得到曹妃甸岸线和岛体动态变化。研究结果显示,曹妃甸岛体面积在近半个世纪发生了较大的变化,由0.34 km2增加为227.34 km2;岸线整体表现为向海推进,1979年曹妃甸陆域岸线25.21%为自然岸线,之后自然岸线比例逐渐减少,自2002年起人工岸线占据了曹妃甸陆域岸线的全部,2012年岛体整体人工岸线长度较1989年自然岸线增长10倍之多;岛体在自然演变时期呈现中部侵蚀、两端淤积、端点延长、西向漂移且..

Research on the long-term variation of zooplankton community in the coastal area of Caofeidian, Hebei Province

基于1959、1984、1990与2004年曹妃甸海域的历史调查资料,分析了曹妃甸海域浮游动物群落的长期变化特征。结果表明:曹妃甸海域浮游动物生物量和丰度整体上呈现增长的趋势,春季尤为显著。2004年春季生物量和丰度分别达到了1093.8 mg/m~3和775.8 ind/m~3,为1959年的4.75倍和5.15倍。浮游动物丰度的季节变化由1959年的夏季高于春季转变为2004年的春季高于夏季的趋势。曹妃甸北部和东北部海域春、夏两季浮游动物生物量和丰度均较高,尤其东北侧龙岛附近海域形成一个极高值区。浮游动物优势种季节变化明显,春季主要为中华哲水蚤(Calanus sinicus)与双毛纺锤水蚤(Acartia bifilosa);夏季以强壮箭虫(Sagitta crassa)为主。此外,水母类浮游动物在夏季明显增多

Phytoplankton community and changes after reclamation in Caofeidian coastal waters

Based on two surveys respectively in August 2013 and May 2014 in Caofeidian coastal waters,phytoplankton community,its correlations with environmental factors,and changes after land reclamation were analyzed.The results showed that totally 49 and 76 species were identified in spring and summer,respectively,in which diatoms dominated with rare dinoflagellates and chrysophytes.Phytoplankton abundance in spring ranged from 1.93×10~4 cells/m~3 to 7.89×10~4 cells/m~3,and dominant species included Paralia sulcata,Coscinodiscus subtilis,etc.; while in summer,it ranged from 5.85×10~4 cells/m~3 to 5022.91×10~4 cells/m~3,and dominant species were Eucampia zodiacus,Skeletonema costatum,etc.The distributions of phytoplankton abundance in spring and summer were similar with high abundances ordinarily observed in inshore waters.Temperature,salinity and nutrients were important factors controlling seasonal shifts and spatial variations of phytoplankton community.After land reclamation,the changes of Shannon-Wiener diversity index(H′) and Pielou eveness index(J) showed spatial variations,which was related to nutrients redistribution due to hydrodynamic changes.The northeastern bay was seriously affected by reclamation.Freshwater and nutrients imported by rivers were hard to exchange outside,thus promoting the proliferation of E.zodiacus whose abundance reached 3725.58×10~4 cells/m~3 in summer,which suggested that the bay tended to be threatened by red tide

Effects of environmental variables on catch rates of Thunnus alalunga in waters near Cook Islands

基于2014年5月的现场调查,从水质、底质和生物三个方面构建了曹妃甸近岸生态系统的健康评价指标体系,利用模糊综合评价法评价曹妃甸近岸海域各站点健康水平和综合健康水平。评价结果显示:底质优于水质和生物,综合健康评价结果为亚健康。采用反距离权重(IDW)空间插值法绘制的水质、底质和生物的健康状况空间分布图以及综合健康水平空间分布图。水质、底质和生物的健康水平空间变化趋势差别较大,综合健康分布图反映出研究区西侧比东侧污染更为严重的现状

河北省曹妃甸近岸海域春季生态系统健康现状评价

基于2014年5月的现场调查,从水质、底质和生物三个方面构建了曹妃甸近岸生态系统的健康评价指标体系,利用模糊综合评价法评价曹妃甸近岸海域各站点健康水平和综合健康水平。评价结果显示:底质优于水质和生物,综合健康评价结果为亚健康。采用反距离权重(IDW)空间插值法绘制的水质、底质和生物的健康状况空间分布图以及综合健康水平空间分布图。水质、底质和生物的健康水平空间变化趋势差别较大,综合健康分布图反映出研究区西侧比东侧污染更为严重的现状

Spatiotemporal change characteristics of Caofeidian shoreline and island in the past three decades

在对1979年、1987年、1991年、1997年、2002年-2005年、2008年、2012年十个时期曹妃甸遥感影像图解译的基础上,从自然地理学角度对岸线和岛体历史演变趋势进行系统分析,得到曹妃甸岸线和岛体动态变化。研究结果显示,曹妃甸岛体面积在近半个世纪发生了较大的变化,由0.34 km2增加为227.34 km2;岸线整体表现为向海推进,1979年曹妃甸陆域岸线25.21%为自然岸线,之后自然岸线比例逐渐减少,自2002年起人工岸线占据了曹妃甸陆域岸线的全部,2012年岛体整体人工岸线长度较1989年自然岸线增长10倍之多;岛体在自然演变时期呈现中部侵蚀、两端淤积、端点延长、西向漂移且..

Spatial and temporal variations of zooplankton community and their influential factors in Caofeidian coastal waters

Based on two surveys conducted in August 2013 and May 2014 in Caofeidian coastal waters, we studied the temporal and spatial variations of species composition, abundance, biomass, dominant species, and the diversity of the zooplankton community; analyzed their relationships with environmental factors; and compared it with an investigation in 2004 to discuss the changes of zooplankton abundance and dominant species and reclamation impacts. A total of 31 species were identified, which were mainly composed of copepod and larvae. Dominant species include Acartia bifilosa, Paracalanus parvus, Oithona similis, and Copepodid larva. Compared to summer, spring exhibited higher values in zooplankton abundance and biomass but lower values in diversity and in the evenness index. Zooplankton spatial distribution of abundance was largely related to seawater temperature and chlorophyll a (Chl-a). Zooplankton abundance showed a roughly decreasing trend from inshore to offshore waters in spring, whereas showing a converse pattern with high values distributed in offshore waters in summer. A comparison with the investigation in 2004 revealed an obvious decline of zooplankton abundance in spring and summer, possibly due to the decrease of dissolved inorganic phosphorus and phytoplankton abundance. The western (eastern) waters showed a decrease (an increase) in zooplankton abundance in spring, which is related to the promoted phytoplankton proliferation induced by nutrient redistribution after reclamation. The study area was mainly impacted by riverine inputs in summer, and the changes in zooplankton abundance showed no obvious spatial variation