A Study of Human Resource Management Outsourcing in Corporation

从二十世纪八十年代开始，随着信息产业技术、知识经济和经济全球化的发展，组织的经营和管理模式发生了很大的变化，业务外包兴起就是这些诸多变化中的一种。作为的一种新型企业经营模式，业务外包能够在世界经济发展不平衡、众多产业不景气的情况下保持持续高速的增长，这不仅引起了企业界的高度重视，也吸引了学术界的广泛关注，并成为近十几年来管理学和经济学研究的热点问题之一。人力资源管理外包是所有业务外包类型当中发展最快的领域之一，而中国在全球业务外包处于领先地位的国家和地区之一。企业的人力资源管理外包选择均出于某种特定的目的和动机。但不同类型的企业在进行人力资源管理外包决策时，其外包的动因会存在着一定的差异；即便...With the development of information industry, knowledge-based economy and economic globalization, great changes have taken place in the patterns of operation and management in corporation since the 1990s. The sustainable, rapid development of the outsourcing industry, in a time of global disequilibrium of economic development, has drawn wide attention not only from the business circle, but also fr...学位：管理学博士院系专业：管理学院企业管理系_企业管理(含财务管理、市场营销、人力资源管理)学号：B20021400

Probe into the Governmental Guiding of the Public Opinion through the Internet —— base on the analysis of Emergency

2010年，中国网民人数首次超过4亿，位居世界第一，与网民数量持续攀升相对应的是，源发于网络曝光的突发事件也大幅增多，有相当比例甚至演化成全国性舆论焦点。面对这种情况，目前大多数政府部门采取的应对措施单一，尤其是在运用互联网引导网络热点事件舆论时，显得缺少有效的方法，甚至有些方法不但不能平息事件，反而“滚雪球”式让事件地越来越复杂，成为讨论不休的公共话题。在网络越来越普及的今天，政府部门在面临突发事件时，应该采取什么方法，克服网络的劣势，最大程度发挥其优势？这项研究显得极为迫切和需要。 本文以文献分析法为主，结合案例分析法、内容分析法、比较研究法等，以传播学为主要理论支撑，借鉴行政管理等领域...In the year of 2010,the number of the Chinese "netizen" has exceeded 400 million, ranking first in the world. Corresponding to this, the emergency dig out by the Internet has been increasing substantially, and a great deal of them has come to the national focus in public. However, quite a few of government departments have no way of doing it when they encounter such a problem. Especially when t...学位：文学硕士院系专业：新闻传播学院新闻学系_新闻学学号：1052008115313

垂体促甲状腺激素瘤的临床特点分析和治疗

目的探讨垂体促甲状腺激素瘤(垂体TSH瘤)导致中枢性甲亢的临床特点及治疗,为临床治疗提供参考。方法回顾性分析5例垂体TSH瘤患者的临床和实验室检查资料,5例均有不同程度的高代谢症状伴或不伴鞍区占位效应,且血清FT4、FT3水平升高,TSH升高或正常,均经垂体磁共振(平扫+增强)检查为大腺瘤。结果 4例行经鼻蝶神经内镜鞍区占位切除术,1例行开颅垂体瘤切除术,术后病理证实均为垂体腺瘤,免疫组化TSH阳性。结论甲亢患者血清TSH不被抑制时要警惕有无垂体TSH瘤的可能,需及时行垂体磁共振检查,若发现垂体腺瘤尽早手术治疗

Comparison of the preparation methods of Temozolomide-loaded nanoparticles

目的比较载替莫唑胺聚氰基丙烯酸正丁酯纳米粒(TMz-PbCA-nP)的不同制备方法,确定最佳制备工艺。方法以α-氰基丙烯酸正丁酯(bCA)为载体,分别采用乳化聚合法和界面聚合法制备TMz-PbCA-nP,加以吐温-80(T-80)进行表面修饰,并通过zETA电位仪检测纳米粒粒径和电位、透射电镜观察纳米粒形态、紫外分光光度计测定各自的包封率和载药量。结果乳化聚合法制备的TMz-PbCA-nP平均粒径(135.8±11.3)nM,表面电位(-24.8±2.2)MV,包封率(44.23±2.04)%,载药量(2.80±0.05)%;界面聚合法制得的载药纳米粒平均粒径(175.4±10.2)nM,表面电位(-18.3±3.6)MV,包封率(44.35±2.58)%,载药量(2.31±0.47)%。透射电镜下观察两种方法所制备的纳米粒大小均较为均匀,粒子间无明显聚集。结论采用乳化聚合法制备TMz-PbCA-nP效果较优于界面聚合法。【Objective】 To compare Temozolomide polybutylcyanoacrylate nanoparticles(TMZ-PBCA-NP) prepared by two different methods so as to determine the optimal process.【Methods】 TMZ-PBCA-NP was prepared by emulsion polymerization and interfacial polymerization methods separately and the surfaces of the two kinds of nanoparticles were both modified with tween-80(T-80).Zeta potential instrument was used to detect the particle size and charge and transmission electron microscopy was employed to study the particle shape.The entrapment efficiency and drug loading were determined with ultraviolet spectrophotometry.【Results】 The mean particle size,surface charge,entrapment efficiency and drug loading of the NP prepared by emulsion polymerization and interfacial polymerization methods was(135.8±11.3) nm and(175.4±10.2) nm,(-24.8±2.2) mV and(-18.3±3.6) mV,(44.23±2.04) % and(44.35±2.58) %,(2.80±0.05) % and(2.31±0.47) %,respectively.Both nanoparticles had uniform size distribution and no apparent aggregation according to transmission electron microscopy.【Conclusion】 The emulsion polymerization method is better than interfacial polymerization method in preparing TMZ-PBCA-NP.国家自然科学基金(No:81172394

Establishment of a nude mouse model of glioma orthotopic xenograft with double-fluorescent labeling

目的建立一种稳定、可实时监测的胶质瘤原位移植瘤裸鼠模型。方法用带有荧光素酶（luciferase-Luc）和绿色荧光蛋白（green fluorescent protein-GFP）基因的慢病毒感染U251神经胶质瘤细胞,流式细胞仪筛选稳定表达GFP-Luc荧光的细胞系,并通过CCK-8实验、细胞周期实验、Transwell肿瘤迁移及侵袭实验等评价荧光细胞的增殖、迁移和侵袭能力是否改变;将细胞接种至裸鼠大脑尾状核,建立胶质瘤原位移植瘤模型,利用小鼠活体成像系统监测脑内肿瘤的生长情况,并通过石蜡切片,HE染色评价细胞在裸鼠脑内的病理特征及成瘤能力。结果成功构建稳定表达GFP荧光和luciferase荧光的U251胶质瘤细胞系及动物模型,慢病毒整合并未改变细胞的增殖、迁移及侵袭能力;模型生长周期适中,成瘤率高,瘤体在颅内生长稳定,HE切片符合人胶质瘤特征。结论双荧光标记的胶质瘤细胞相比于传统细胞更有利于胶质瘤动物模型的实验研究;U251-GFP-Luc胶质瘤细胞裸鼠模型,其肿瘤生长和病理特性与人胶质瘤相似,且可实时观察肿瘤生长,可作为胶质瘤实验研究的理想动物模型。Objective To establish a stable and real-time monitorable nude mouse model of orthotopic glioma xenograft. Methods U251 glioma cell line was infected by a leutiviral vector containing green fluorescent protein （GFP） and luciferase （Luc） gene. Ceils stably expressing fluorescence of GFP and Luc were sorted by flow cytometry. CCK-8 test and Transwell tumor invasion and migration assay were used to compare the biological features between the ceils stably expressing GFP-Luc fluorescence and cells without fluorescence. Then the ceils were implanted intracranially in the right caudate nucleus of athymic BaIb/c nude mice to establish the tumor model. The growth of intracerebral tumor was monitored over time by a bioluminescence imaging （BLI） system. Hematoxylin-eosin （HE） staining was used to evaluatethe histopathological features and tumorigenicity of the transplanted glioma cells in the brain of nude mice. Results U251 glioma cell line with stably expressing GFP-Luc fluorescence and the corresponding orthotopic xenograft model were successfully established. There was no statistically significant difference in the proliferation, invasion and migration abilities between the cells with stably expressing GFP-Luc fluorescence and the control cells. This model showed a high tumor formation rate and stable tumor growth, and takes a moderate time to establish this model. Conclusions Compared with the traditional glioma cells, GFP-Luc-transfected human glioma cells are more feasible for the studies of glioma in vivo. The tumor growth and pathological characteristics in this U251-GFP-Luc glioma model are similar to human glioma, and the growth of this tumor can be real-time monitored. It can be used as an ideal animal model for experimental studies of glioma.国家自然科学基金（编号：30970733）

Traumatic superficial temporal artery aneurysm:analysis of 7 cases

目的探讨外伤性颞浅动脉瘤的诊断和治疗方法。方法回顾性分析7例外伤性颞浅动脉的临床表现、手术方法和治疗结果。所有病例均因外伤后额部或颞部进行性增大肿物就诊,无颞浅动脉瘤破裂出血病例,全部病例在局部麻醉下切除动脉瘤囊肿,其中1例为分叉部梭形动脉瘤。结果病理报告均为假性动脉瘤,动脉外膜存在。无术后头皮缺血、坏死发生。结论根据外伤史、颞浅动脉走行上搏动性肿物的特点,颞浅动脉瘤即可明确诊断。动脉壁中膜弹力层断裂,局部因动脉血流搏动冲击膨出形成可能是颞浅动脉瘤形成的另一个机制。动脉瘤切除术是治疗颞浅动脉瘤的有效方法。Objective To explore the diagnosis and treatment for traumatic superficial temporal artery pseudoaneurysm.Methods Clinical manifestations, operation and prognosis of 7 patients with traumatic superficial temporal artery pseudoaneurysm were analyzed retrospectively.There was the same complaint of progressive swelling mass on the frontal or temporal area post brunt injury, but no complaint of rupture of the aneurysm in all the patients.All the patients underwent resections of the aneurysm capsule under local anesthesia, including one fusiform aneurysm at the bifurcation of the superficial temporal artery in 1 case.Results All the cases were pseudoaneurysm with intact outlayer of the artery.There was no complication of scalp ischemia or necrosis.Conclusion Traumatic superficial temporal artery pseudoaneurysm can be diagnosed according to the history of head injury and progressive pulsatile swelling or mass on the line of the superficial temporal artery.It may be another mechanism of forming pseudoaneurysm that breakdown of the elastic layer in the tunica media of the arterial wall and beating of arterial blood flow arterial lead to lumen enlargement on the local point.Resection of the aneurysm is an effective way of the treatment

Preparation of fotemustine-loaded polybutylcyanoacrylate nanoparticles

目的优化工艺制备福莫司汀聚氰基丙烯酸正丁酯纳米粒(fCnu-PbCA-nP)。方法以α-氰基丙烯酸正丁酯(bCA)为载体,采用乳化聚合法制备fCnu-PbCA-nP,并加以聚乙二醇20000(PEg20000)进行表面修饰,通过考察粒径和包封率两个指标,在单因素实验初选的基础上,正交设计法优化处方和制备工艺。结果制备fCnu-PbCA-nP的优化条件为bCA单体体积分数0.8%(V/V)、fCnu 20 Mg、PEg20000浓度2.0%,按优化条件所制备的fCnu-PbCA-nP的粒径为(124.6±5.2)nM,多分散系数(PdI)范围为0.07--0.16,包封率(64.12±2.36)%,载药量(7.28±0.76)%。结论通过优化处方和制备工艺,采用乳化聚合法可制备出fCnu-PbCA-nP,对拓展fCnu临床给药新剂型提供一定的参考。AIM To prepare fotemustine polybutylcyanoacrylate nanoparticles(FCNU-PBCA-NP) with optimized process.METHODS FCNU-PBCA-NP was prepared by emulsion polymerization with the α-butylcyanoacrylate(BCA) as its carrier and the surface of the nanoparticles was modified with polyethylene glycol 20000(PEG20000).Single factor test and orthogonal design were carried out to optimize the preparing technology according to the particle size and the entrapment efficiency of FCNU-PBCA-NP.RESULTS The optimal conditions for the preparation of FCNU-PBCA-NP were 0.8% BCA monomer(V/V),20 mg fotemustine and 2.0% PEG20000(m/V).On the basis of the above conditions,the mean particle size of the NP was(124.6±5.2)nm and the polydispersity index(PDI) was 0.07-0.16,the average entrapment efficiency and drug loading was(64.12±2.36)% and(7.28±0.76)%,respectively.CONCLUSION An optimized nanoparticle drug delivery system is obtained by emulsion polymerization and provides a new direction for fotemutine dosage forms in future.福建省自然科学基金(2006J0188);厦门市科技局基金(3502Z20064013

基于生物信息学途径探究载脂蛋白 H 在肝衰竭中的功能及作用机制

【目的】通过生物信息学方法，分析在肝衰竭中高表达的载脂蛋白H（APOH）在疾病发生发展中的功能与机制。【方法】从GEO数据库下载多个芯片数据集（GSE14688、GSE38941和GSE96851），以5筛选出差异表达基因，通过Cytoscape及R对前10位关键基因中的APOH基因进行GO生物学功能富集分析和KEGG信号通路分析，并进一步在慢乙肝相关肝衰竭组织中进行表达验证。【结果】共筛选出2438个差异表达基因，其中，1162个上调基因，1276个下调基因。蛋白质相互作用网络分析发现10个枢纽基因分别为：KNG1、IGF1、SPARC、APOH、CLU、SERPING1、TGFB2、CDC37L1、PCYOX1L和APOOL。通过慢乙肝相关肝衰竭组织验证发现APOH在肝衰竭组织中高表达，GeneMANIA预测APOH和炎症反应有关。GO分析及KEGG分析显示，APOH与补体/凝血级联通路以及碳代谢通路密切相关，并且，与补体C3（complement C3）的表达水平呈明显的正相关性。【结论】APOH在肝衰竭组织中高表达，可能通过补体C3调控补体/凝血级联通路以及碳代谢通路参与肝衰竭的发生发展

Optimization of Fermentation Conditions of Marine Algicidal Bacterium Microbulbifer sp. BS03 by Uniform Design

通讯作者 Corresponding author (E-mail: [email protected]; [email protected])[中文文摘]采用均匀设计法设计和二次多项式逐步回归分析,对一株高效杀塔玛亚历山大藻微泡菌BS03(Microbulbifer sp.)产杀藻活性物质的发酵培养条件进行优化.通过单因素实验筛选出碳源、氮源、pH、培养时间和接种量为显著影响因子,并对5个显著影响因子采用U15(155)水平对培养基进行优化.结果表明BS03最适发酵培养条件为:蔗糖8 g/L,蛋白胨10.50 g/L,初始pH值7.5,培养时间32 h,接种量3.00%.验证试验结果显示,在此条件下该菌发酵液的干重为4.725 g/L,较优化前增加了31.35%,LD50为0.768%,较优化前降低了25.14%.研究结果为杀藻活性物质以及杀藻机理的研究奠定了理论基础.[英文文摘]The optimal parameters of fermentation conditions of marine bacterium Microbulbifer sp.BS03 producing algicidal substances against Alexandrium tamarense were obtained by using Uniform Design and quadric polynomial regression methods.Five main factors were achieved through single factor experiments,that is,carbon source,nitrogen source,pH,incubation time and inoculum size.The U15(155) design result showed the optimum conditions of experiment were as follows: sugar 8 g/L,peptone 10.5 g/L,initial pH 7.5,incubation time 32 h, and inoculum size of 3.00%. According to the verifi cation experiment, under the optimal conditions, the dry biomass was 4.725 g/L which was increased by 31.35% and LD50 was 0.768% which was decreased by 25.14% compared with those of the basic fermentation conditions. This outcome will help us to separate active substances from complex components in medium and thus to reveal the mechanism of algicidal activity in the future. Fig 7, Tab 5, Ref 20.国家自然科学基金项目(Nos.40930847,31070442); 福建省自然科学基金项目(Nos.2012J01150,2010J01223);; 福建省教育厅项目(No.JA10232);莆田市科技计划项目[No.2011S09(4)]资助