pJAK2 polypeptide,an antagonist of suppressors of cytokine signaling-1,can enhance the antitumor effect of dendritic cells

目的:探讨细胞因子信号转导抑制因子-1(SuPPrESSOrS Of CyTOkInE SIgnAlIng1,SOCS1)拮抗物PJAk2多肽(氨基酸序列号为1001-1013)参与树突状细胞(dEndrITIC CEllS,dCS)的体外诱导培养后对dCS抗肿瘤作用的影响。方法:采集健康人外周血,离心获得单个核细胞,用重组人粒细胞巨噬细胞集落刺激因子(rECOMbInAnT HuMAn grAnulOCyTE-MACrOPHAgE COlOny STIMulATIng fACTOr,rHgM-CSf)及重组人白细胞介素-4(rECOMbInAnT HuMAn InTErlEukIn-4,rHIl-4)诱导dCS,第5天分为4组:单纯dCS培养(对照)组、抗原负载(lySATE-dCS)组、多肽修饰(PJAk2-dCS)组和抗原与多肽共培养(lySATE+PJAk2-dCS)组,第6天各组加入肿瘤坏死因子-α(TuMOr nECrOSIS fACTOr-AlPHA,Tnf-α)促成熟。倒置显微镜下观察dCS形态;fCM法检测dCS表型;乳酸脱氢酶(lACTATE dEHydrOgEnASE,ldH)细胞毒实验检测各组细胞毒性T淋巴细胞(CyTOTOXIC T lyMPHOCyTE,CTl)对胃癌细胞bgC-823的靶向杀伤作用;ElISA法检测白细胞介素-12(InTErlEukIn-12,Il-12)和γ干扰素(InTErfErOn-γ,Ifn-γ)的水平。结果:与未加入诱导剂组相比,各组均成功诱导出成熟dCS,均高表达Cd80、Cd83、Cd86和人类白细胞dr抗原(HuMAn lEukOCyTE AnTIgEn dr,HlA-dr),但以lySATE+PJAk2-dCS组的表达水平最高。在10:1~30:1的效靶比范围内,CTl杀伤作用与效靶比呈正相关。当效靶比为30:1时,对照组的CTl杀伤率达(19.77±2.34)%,低于其他3组(P<0.01),而lySATE+PJAk2-dCS组较lySATE-dCS组及PJAk2-dCS组都高(P<0.05)。lySATE+PJAk2-dCS组培养上清液中Il-12及Ifn-γ的分泌水平明显高于对照组(P<0.01)。结论:SOCS1拮抗物PJAk2多肽(1001-1013)可增强dCS对胃癌细胞的抗原递呈及特异性抗肿瘤作用。Objective:To investigate the effect of pJAK2 polypeptide,an antagonist of SOCS1(suppressors of cytokine signaling 1),on antitumor effect of in vitro cultivation-induced DCs(dendritic cells).Methods:Peripheral blood was collected from the healthy volunteers,and the PBMCs(peripheral blood mononuclear cells)were isolated.DCs were induced by rhGM-CSF(recombinant human granulocyte-macrophage colony-stimulating factor)and rhIL-4(recombinant human interleukin-4).On the fifth day,DCs were divided into four groups:control group,Lysate-DCs group,pJAK2-DCs group,and Lysate + pJAK2 DCs group.On the sixth day,TNF-α(tumor necrosis factor-alpha)was added into each group.The morphological features of DCs were observed under an inverted microscope;the phenotypes were detected by FCM(flow cytometry);the killing effect of CTLs(cytotoxic T lymphocytes)on gastric cancer BGC-823 cells was evaluated by LDH(lactate dehydrogenase)cytotoxicity test;the concentrations of IL-12(interleukin-12)and IFN-γ(interferon-γ)were detected by ELISA(enzyme-linked immuno sorbent assay).Results:Mature DCs presented typically morphological and phenotypic features;the DCs in Lysate + pJAK2-DCs group had the highest expression levels of CD80,CD83,CD86 and HLA-DR(human leukocyte antigen DR).When the ratio of effectors to target cells ranged from 10:1 to 30:1,the killing activity of CTLs had a positive correlation with the ratio.When the ratio of effectors to target cells was 30:1,the killing activity of CTLs in the control group was(19.77±2.34)%,which was lowest as compared with the other groups(P < 0.01),meanwhile the killing activity of CTLs in Lysate + pJAK2-DCs group was higher than those in Lysate-DCs and pJAK2-DCs groups(P < 0.05).The levels of IL-12 and IFN-γ secretion in Lysate + pJAK2-DCs group were apparently higher than those in the control group(P < 0.01).Conclusion:An antagonist of SOCS1,pJAK2 polypeptide,can enhance the ability of antigen presentation and specific antitumor effect of DCs on gastric cancer cells.南京军区医学科技创新课题资助项目(编号:10MA068); 福建省自然科学基金面上项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014

Major QTLs Mapping and Analysis for Rice Grain Chalkiness and Grain Shape Traits

本研究用珍佳b(佳辐占/珍汕97b//珍汕97b的回交重组自交系f11,即bC1f11)x珍汕97b的f2群体,对稻米粒长、粒宽、长宽比、粒厚和垩白粒率性状进行遗传分析与QTl定位。结果表明,粒宽、长宽比、粒厚和垩白粒率均属于由多基因控制的数量性状,而粒长受一个主效基因控制。共检测到13个控制糙米粒长、粒宽、长宽比、粒厚和垩白粒率的QTlS。其中,在第3号染色体着丝粒附近rM16-rM411区间同时控制粒长、粒宽、长宽比和粒厚性状,遗传贡献率分别为49.8%、12.6%、39.3%和5.3%;在第5号染色体着丝粒附近rM7118-rM3683区间同时控制垩白粒率、粒宽、长宽比和粒厚性状,遗传贡献率分别为43.9%、44.5%、28.0%和15.0%;同时,在rM169-rM289区间也同时控制垩白粒率、粒宽、长宽比和粒厚性状,但各性状的遗传贡献率均较rM7118-rM3683区间的小。The F2 population was derived from the cross ZS97B (Zhenshan97B)×ZJB (ZhenjiaB) which was the backcross recombinant inbred line from Jiafuzhan×ZS97B.Based on the F2 population, the genetic analysis and QTL mapping of grain appearance quality traits, included grain length, grain width, grain shape (grain length-width ratio), grain thickness and PGWC (percentage of grain with chalkiness), was conducted.Grain width, grain shape, grain thickness and PGWC, belonged to the quantitative trait inheritance.But grain length showed the polygenic inheritance with a major gene.13 QTL associated with grain appearance quality traits were detected in this study.RM16-RM411 located in the pericentromeric region of rice chromosome 3 could explain 49.8%, 39.3%, 12.6%, 5.3% of the phenotypic variation, respectively, for grain length, grain shape, grain width and grain thickness.RM7118-RM3683 located in the centromeric region of rice chromosome 5 was associated with PVE (phenotypic variation explained) of 43.9%, 44.5%, 28.0% and 15.0%, respectively, for PGWC, grain width, grain thickness and grain shape.In addition, another pleiotropic QTL located between RM169 and RM289 on rice chromosome 5 was associated with less PVE for PGWC, grain width, grain thickness and grain shape than RM7118-RM3683.福建省科技创新平台建设计划项目(2007N2005);福建省科技重大专项(2008NZ0001);福建省自然科学基金项目(2011J01249);中央高校基本科研业务费专项资金(2010121088);厦门市科技计划项目(3502Z20083003)共同资

Effect of Helicobacter pylori on the function of peripheral blood monocyte-derived dendritic cells in gastric cancer patients

分析HP阳性和HP阴性的胃癌患者外周血单核细胞来源树突状细胞(MOdCS)功能的差异性及其临床意义。方法:用尿素14C呼气试验对解放军第一七四医院2011年1月至2012年10月收治的84例胃癌患者进行HP感染状态鉴定,分别采集HP阳性和阴性胃癌患者外周血,分离外周血单个核细胞,采用经典方法(rHgM-CSf、rHIl-4联合rHTnf-α)诱导产生dCS,采用流式细胞仪检测dCS表型,采用ldH释放法检测dCS致敏T细胞对胃癌细胞的毒性杀伤作用,采用ElISA方法检测细胞因子Il-12、Ifn-γ的分泌水平。结果:两组MOdCS成熟过程形态变化无差异,HP阳性组MOdCS表面标记分子Cd1A、Cd80、Cd83、Cd86和HlA-dr平均表达百分率均高于HP阴性组,其中Cd80、Cd83、Cd86的表达水平差异有统计学意义,Cd1A、HlA-dr差异无统计学意义。HP阳性组dCS致敏T淋巴细胞对胃癌细胞杀伤率和Il-12、Ifn-γ的分泌水平均高于HP阴性组(P<0.05)。结论:HP感染状态不影响胃癌患者外周血MOdCS成熟过程形态变化,HP感染的胃癌患者MOdCS成熟和活化水平更高。Objective:This study aimed to compare and analyze the functional differences between peripheral blood monocyte-derived dendritic cells(DCs) of Helicobacter pylori-positive and H.pylori-negative patients with gastric cancer.Methods:H.pylori infection was detected in 84 patients with gastric cancer in our hospital from January 2011 to October 2012 by the14C-urea breath test.DCs were generated from monocytes isolated by an adherent method from the two groups of patients and cultured in the presence of rhIL-4,rhGM-CSF,and rhTNF-α.Furthermore,the expression of surface marker molecules was determined by fluorescence-activated cell sorting analysis.The cytotoxicity of DCs pulsed T cells against gastric carcinoma cell was assessed by the lactate dehydrogenase-releasing assay.The secretion of IL-12 and IFN-γ in the supernatant was determined by enzyme-linked immunosorbent assay.Results:No difference was observed in the morphological change of the maturation process.The mean expression of CD1a,CD80,CD83,CD86,and HLA-DR molecules in DCs of H.pylori-infected patients was higher than that in DCs of H.pylori-negative group,and the differences were statistically significant except for CD1a and HLA-DR.The cytotoxicity activities,IL-12 release,and IFN-γ release in the H.pylori-positive group were significantly higher than those in the H.pylori-negative group(P<0.05).Conclusion:H.pylori infection has no effect on the morphological change of the maturation process of monocyte-derived DCs.These data clearly demonstrate that monocyte-derived DCs of H.pylori-infected patients with gastric cancer can induce stronger maturation and activation than those of H.pylori-negative patients.南京军区医学科技创新课题(编号:10MA068); 福建省自然科学基金资助项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014)资助~

Erosive rainfall and rainfall erosivity in purple hilly area

&nbsp;降雨侵蚀力(R值)的空间分布反映了区域气候对土壤侵蚀的作用。利用四川盆地紫色丘陵区多年实测降雨资料,应用频率分析法,推求该地区侵蚀性降雨的一般雨量标准,揭示该地区侵蚀性降雨及其侵蚀特征,进而运用降雨侵蚀力日降雨量计算方法,分析紫色丘陵区降雨侵蚀力时空分布特征。结果表明:1)紫色丘陵区顺坡休闲农耕地的侵蚀性降雨的一般雨量标准为11.3 mm;2)紫色丘陵区多年平均总降雨量中有60%以上属于侵蚀性降雨,侵蚀性降雨主要集中于5&mdash;9月,其中7、8月年均侵蚀性降雨量和土壤侵蚀量最大,空间分布上表现为丘陵区边缘地区大于中部地区;3)紫色丘陵区年均R值介于5 000～6 500 MJ/(mm.hm2.h)之间,由丘陵区周边向中心逐渐减小,研究区北部的巴中、达县、阆中3站的年均降雨侵蚀力形成高值区,中部的遂宁站形成低值中心,北部大于南部,西部大于东部;4)紫色丘陵区R值主要由&ge;15 mm的降雨构成,占76.9%～82.1%,年内集中度较高,主要分布在汛期5&mdash;10月份,占年R值的89%以上;5)R值的年际变化较大,达到中等程度变异,不同地区的R值年际变化差异较大,但并未表现出明显的随时间变化的增减趋势</span

Method of deducing soil erosion amount of drainage basin with sediment discharge

鉴于至今还没有一个有效获取流域侵蚀产沙量的方法,在分析悬移质泥沙与侵蚀泥沙之间的数量与粒配关系的基础上,阐述了应用输沙量推演流域侵蚀量在理论上的可能性,但却存在着难点,且主要表现在水文站数量不足与区域分布不均、流域内的生态环境复杂与区域差异大、水文站输沙量观测记录起始时间不同步、及新技术应用存在时空条件的局限性。结合与侵蚀产沙和泥沙输移比相关的定性与定量指标,提出了应用输沙量推演流域侵蚀量的方法,即&quot;层次类剔法&quot;,来计算与评估流域侵蚀量和泥沙输移比,具体的操作过程是首先制作一幅流域地貌类型地块图,然后是逐类型、逐类型地块的侵蚀量计算和沟道流域泥沙输移比的判断,最终获取流域不同地块的侵蚀强度和流域泥沙输移比。本研究结果可为水土流失监测点的选择提供科学依据,同时对解决流域的泥沙问题也有重要的启迪作用

Responses of Root and Leaf NR Activity in Wheat Seedling to Elevated CO_2 Concentration under Different N Levels

以小麦品种&lsquo;小偃22&rsquo;幼苗为材料,采用开顶式气室和水培实验研究了不同供氮水平(2.5、5.0、10.0和15.0 mmol.L-1)下小麦幼苗植株生长量、根系形态、有机碳分泌速率和硝酸还原酶(NR)活性对大气CO2浓度升高的响应。结果显示,大气CO2浓度倍增均增加了小麦幼苗各生长阶段根冠生物量以及根系长度、面积、有机碳分泌速率和叶片NR活性。随供氮水平的提高,各生长阶段幼苗根冠生物量、根长和面积以及叶片NR活性呈上升趋势,而有机碳分泌速率呈下降趋势;根冠比变化不同阶段表现不一致,一叶一心期呈下降趋势,二叶一心期和三叶一心期分别以15.0和10.0 mmol.L-1氮水平较高。研究表明,大气CO2浓度升高可促进小麦幼苗根系生长和有机碳分泌速率,提高其氮素同化能力;增加介质供氮有利于高CO2浓度条件下小麦幼苗根冠生长和氮素同化,提高根冠比,减少根系有机碳过度分泌引起的碳损耗