Study on Embryotoxicity and Bioaccumulation of the Combined Exposure of Typical Antibiotics in Oryzias Melastigma Embryos

抗生素以其优良的抗菌作用，被人类广泛地使用，致使其以各种途径，大量地进入到自然环境中，尤其是水环境中。其中，磺胺类和氟喹诺酮类药物因其广谱的抗菌性而被大量使用，因此成为水环境中检出率最高、检出浓度最大的两类抗生素。目前，环境中抗生素的研究主要集中于痕量检测，对于水生生物毒性研究多针对于大型溞、浮萍、月芽藻等低等水生生物，而对低剂量、长期暴露下抗生素对鱼类的生理毒性效应，及其在生物体内的富集研究相对较少，其在水环境中潜在的生态毒理效应尚未有明确的评价，有必要对相关的生态毒理数据进行完善，同时，研究手段上偏重生物学方法，与化学相结合来研究毒性作用机制非常欠缺。 本研究从磺胺类和氟喹诺酮两大类抗生...Antibiotics have been widely used by people with its excellent antibacterial effect, which result in a large number of antibiotics into the environment through a variety of ways, especially water environment. The sulfonamides and fluoroquinolones were widely used, and this has caused that they became one of the highest detection rate and quantity antibiotics. Currently, most reseach focus on trace...学位：理学硕士院系专业：海洋与地球学院_海洋化学学号：2232012115130

Another chiral crystal structure of 1-[4-(Dimethylamino)benzylidene]-4-phenylthiosemicarbazide

Another chiral crystal of 1-[4-(dimethylamino)benzylidenel-4-phenylthiosemicarbazide (DMB) was obtained, and its absolute structure was determined by X-ray single crystal diffraction and the solid circular dichroism (CD) spectrum. The crystal belongs to orthorhombic system with space group P2(1)2(1)2(1) and a = 0.7870(2), b = 1. 1560(2), c = 1.6668(3) nm(3), V = 1.5164(5) nm, Z= 4, D-c=1.307 g/cm(3), F(000)=632, mu=0.213 mm(-1), the final R=0.0409 and wR=0.1061 for 2577 observed reflections [I > 2 sigma(I)]. The absolute structure can be determined well with the Flack parameter 0.00(9). The crystal structure and CD spectrum show that an enantiomer of the compound packs into the chiral crystal

1-[4-(二甲氨基)苯亚甲基氨基]-4-苯基硫脲的另一个手性对映体晶体结构

对-二甲氨基苯甲醛和苯基氨基硫脲缩合反应生成1-[4-(二甲氨基)苯亚甲基氨基]-4-苯基硫脲(DMB),产物能从溶液中析出单一手性对映体晶体.用单晶X射线衍射技术测定了它的绝对构型,晶体属正交晶系,P212121空间群,a=0.7870(2)nm,b=1.1560(2)nm,c=1.6668(3)nm,V=1.5164(5)nm3,Z=4,Dc=1.307g/cm3,F(000)=632,μ=0.213mm-1,2557个可观测点[I>2σ(I)]精修的最终残差因子:R=0.0409,wR=0.1061,Flack参数为0.00(9),能够确定绝对构型.化合物的晶体结构和大宗粉末样品的固体圆二色谱表明化合物在结晶过程中发生单一对映体的手性堆积

Traditional Chinese Medicine nursing management for patients with sequelae of pelvic inflammatory disease (盆腔炎性疾病后遗症中医护理探讨)

Pelvic inflammatory disease is an infection of a woman’s reproductive organs, which can lead to a series of sequelae if not properly treated. Sequelae of pelvic inflammatory disease may seriously threaten the health of women and increase financial burden to the family and society. The syndrome of sequelae of pelvic inflammatory disease can be differentiated into four types: dampness-heat attacking lower energizer, Qi stagnation and blood stasis syndrome, cold-dampness stagnation syndrome and Qi deficiency and blood stasis syndrome. The main Traditional Chinese Medicine nursing techniques have been applied are Intermediate frequency electrotherapy, ultrasound drug penetration, acupoint application, Traditional Chinese Medicine soaking, Traditional Chinese Medicine enema, auricular point sticking, Traditional Chinese Medicine cupping therapy and so on. Based on the syndrome differentiation, a set of nursing intervention including body posture nursing, emotional nursing, medication service, symptom assessment, dietary instruction, exercise instruction, defecation instruction, etc, is required to improve the rehabilitation of patients. (盆腔炎性疾病是指女性上生殖道及其周围组织的一组感染性疾病, 若未得到及时正确的治疗, 可导致一系列的后遗症, 即盆腔炎性疾病后遗症。盆腔炎性疾病后遗症严重影响妇女健康, 增加家庭和社会的经济负担。本病中医辨证分为湿热瘀结证、气滞血瘀证、寒湿凝滞证、气虚血瘀证4型, 应用的主要中医护理技术包括中频电疗、超声药物透入、穴位贴敷、中药泡洗、中药灌肠、耳穴埋豆、拔罐疗法等。同时, 治疗期间应开展辨证施护, 包括体位护理、情志护理、服药护理、症状评估、辨证饮食指导、活动指导、排便指导等。

Auricular acupoint pressing therapy and nursing management for insomnia in a patient with central retinal artery occlusion (耳穴压丸治疗1例视网膜中央动脉阻塞患者失眠的护理体会)

This paper summarized the auricular acupoint pressing therapy an nursing measures for insomnia in a patient with central retinal artery occlusion. On the basis of nursing risk assessment, Traditional Chinese Medicine auricular acupoint pressing therapy was adopted. Comprehensive nursing interventions such as strengthening cooperation between medical and nursing care, providing oxygen therapy according to the doctor's advice, enhancing the psychological care and diet care of patients were carried out during the treatment. Traditional Chinese Medicine auricular acupoint pressing therapy can effectively relieve the psychological stress and improve the sleep quality of the patient. (本文总结1例采用耳穴压丸治疗视网膜中央动脉阻塞(CRAO)后失眠的护理经验。在完善护理评估的基础上, 采用中医耳穴压丸治疗, 同时积极配合医生进行救治, 遵医嘱及时给予安全的氧疗护理, 加强患者的心理护理和饮食护理, 有助于缓解患者心理压力, 改善睡眠质量。

广州市5岁以下腹泻患儿感染肠致病性大肠埃希菌的流行特征

目的了解广州市5岁以下儿童感染肠致病性大肠埃希菌(Enteropathogenic Escherichia coli,EPEC)的流行特征和临床表现,为我国儿童EPEC感染的防治提供依据。方法在广州市3家哨点医院收集5岁以下腹泻儿童粪便标本745份,非腹泻儿童粪便标本275份,进行细菌培养。选择单个菌落,提取DNA,采用荧光定量PCR检测EPEC。同时使用结构化问卷收集研究对象基本信息。结果腹泻患者儿中EPEC检出率为4.64%(35/754,95%CI:3.35- 6.39),其中急性腹泻患儿EPEC检出率为5.78%(27/467,95%CI:4.00-8.28),慢性腹泻患儿EPEC检出率为2.85%(8/281,95%CI:1.45-5.52),非腹泻患儿EP EC检出率为1.45%(4/275,95%CI:0.56-3.67),EPEC检出率在腹泻患儿高于非腹泻患儿(χ~2=5.746,P 0.05,OR=1.335,95%CI:0.63-2.822);按各年龄组分组统计,0岁~、1岁~、2岁~、3岁~、4岁~组腹泻患儿EPCE检出率差异有统计学意义(χ~2=16.767,P 0.05,OR=1.148,95% CI:0.491-2.686)。腹泻患儿EPEC阳性者腹泻天数(4,95%CI:3-7)与EPEC阴性者腹泻天数(6,95%CI:5-7)比较,差异无统计学意义(Z=0.414,P>0.05)。结论在广州市城区, EPEC是引起儿童腹泻的重要细菌性病原体之一,流行程度高,且无特异临床表现,容易造成漏诊和误诊。实验室检测可为EPEC相关腹泻的诊治提供参考