Immunohistochemical Study of Serotonin in the Digestive System of Scylla serrata

【中文摘要】 应用免疫组织化学方法对锯缘青蟹消化系统的 5- HT分泌细胞的形态和分布进行了观察 .结果表明 :5- HT阳性细胞形态多样 ,多数为卵圆形 ,少数为柱形 ,多边形或具有较长的胞突 .消化道各段均有 5- HT阳性细胞 ,中肠密度最高 ,食道次之 ,贲门胃、幽门胃和后肠较低 .5- HT阳性细胞多数位于固有膜和粘膜下层 ,少数散布于肌层 .肝胰腺也具有 5- HT阳性细胞 【英文摘要】 The morphology and distribution of serotonin(5 HT) immunoreactivity in the digestive system of Scylla serrata were investigated. 5 HT immunoreactive cells had various forms: the majority were oval, while the minority were columnar, irregular in shape, or having long processes. 5 HT immunoreactive cells were distributed throughout the digestive tract, with the highest density in the midgut, the second in the esophagus, and a low density in the stomach and hindgut. Most of the 5 HT immunoreactive c...福建省重中之重“福建省海洋生物优良种质和生物活性物质的应用基础研究”资助项

Immunorecognition of Estrogen Receptor in the Optic Ganglion of Scylla serrata

【中文摘要】 利用免疫细胞化学SABC法对锯缘青蟹(Scylla serrata)视神经节雌激素受体(ER)进行定位研究.结果表明:视神经节的视外髓、视内髓和视端髓存在ER免疫阳性细胞.视神经节存在的ER免疫阳性细胞可以为雌激素提供结合位点.本研究为雌激素参与视神经节神经内分泌调节作用提供了形态学依据. 【英文摘要】 Antisera generated in vertebrate and immunocytochemical SABC method was applied to observe the immunoreactive neurons and neuropils of estrogen receptor(ER) in optic ganglia of Scylla serrata.The results showed that:ER-immunoreactive substance was located in medulla externa,medulla interna and medulla terminalis in optic ganglion.ER-immunoreactive neurons in optic ganglia might provide the binding sites for estrogen and accept the feedback regulation of estrogen.This present work provides the morphological ...国家自然科学基金(30300269)资

拟穴青蟹受精膜形成过程中环形颗粒的发生与胞吐

为了揭示拟穴青蟹受精膜形成机制,采用透射电子显微镜研究了其受精膜形成过程中环形颗粒的形成与胞吐。结果显示,拟穴青蟹精子入卵后,发生皮层反应而形成受精膜。皮层反应包括致密颗粒首先胞吐以及环形颗粒的相继多轮胞吐。每轮环形颗粒的胞吐参与到受精膜的平缓期、增厚期和举起期3个阶段。环形颗粒的形成主要发生在受精膜的增厚期,受精卵的皮层和靠近皮层的内质中都在形成大量的环形颗粒,并且迅速移到皮层质膜附近发生爆发性胞吐,参与到受精膜的快速形成。环形颗粒的形成与2种卵黄颗粒以及脂滴密切相关,在皮层中发现了线粒体参与其中,推测在拟穴青蟹受精卵的皮层和内质可能存在环形颗粒合成的两套不同机制。本文证实了卵黄颗粒和脂滴及环形颗粒在拟穴青蟹受精膜形成过程中发挥重要作用。国家“八六三”高技术研究发展计划(2002AA603013);;福建省教育厅科技项目(JAT160256);;集美大学学科建设基金(C517064

锯缘青蟹前脑神经分泌细胞的超微结构

经透射电镜观察 ,锯缘青蟹 (Scyllaserrata)前脑具有3种类型的神经分泌细胞。Ⅰ型细胞最大 ,胞质中存在许多大小不同的空泡 ,分泌颗粒数量很少 ;Ⅱ型细胞中等大小 ,细胞器发达 ,分泌颗粒数量较多 ,形态多样 ;Ⅲ型细胞最小 ,分泌颗粒数量最多 ,电子密度低 ,细胞器很少。锯缘青蟹前脑神经分泌细胞的特点 ,为甲壳动物系统演化处于较低的地位提供了形态学证据。 【英文摘要】 Three types of neurosecretory cells in the protocerebrum of Scylla serrata were observed using the transmission electron microscope.TypeⅠcells are50～120μm in diameter with many vacuole and few neurosecretory granule.TypeⅡcells are30～60μmin diameter with developed organelle and various neurosecretory granule.and TypeⅢcells are15～20μm in diameter with feworganelle and abundant neurosecretory granule of electron-sparse.The characters of the neurosecretory cells in the protocerebrum of Scylla serrata provide th...福建省重中之重项目“福建省海洋生物优良种质和生物活性物质的应用基础研究” ;; 高等学校博士学科点专项科研基金项目20010384010号

Histological Studies on Testes Development of the Mud Crab,Scylla serrata

组织学观察表明 ,锯缘青蟹 (Scyllaserrata)精巢发育周期可以分为精原细胞期、精母细胞期、精子细胞期、精子期和休止期 5个时期。在划分精巢发育期的基础上 ,结合生精小管直径和成熟节段比例这 2个指标 ,可以较准确地反映锯缘青蟹精巢的发育状况。而成熟节段比例可以作为锯缘青蟹精巢发育的表征指标。 【英文摘要】 The testicular development cycle of Scylla serrata can be divided into 5 periods:the spermatogonium stage,the spermatocyte stage,the spermatid stage,the sperm stage and the resting stage.Based on the division of the development cycle,the diameter of the seminiferous tubule and the ratio of its mature sections can reflect the process of the testicular development.For the first time,the ratio of the mature sections is presented as a characteristic index of the testicular development.福建省重中之重项

Immunocytochemical localization of serotonin and neuropeptide Y in the brain of Scylla serrata

【中文摘要】 为探讨5-羟色胺和神经肽Y在锯缘青蟹脑内的存在与否和分布状况,应用免疫细胞化学技术,在光学显微镜下观察5-羟色胺和神经肽Y阳性细胞和神经髓质的形态和分布。结果表明:在锯缘青蟹脑中共12个胞体群和11个神经髓质中,前脑和中脑中有4个细胞群和6个神经髓质检出5-HT免疫阳性反应;中脑和后脑中有7个细胞群和3个神经髓质具有NPY免疫阳性反应。5-羟色胺和神经肽Y在锯缘青蟹脑内的特异性分布,为其参与神经生理活动提供了形态学证据。 【英文摘要】 [KG*5]:To explore the locations of serotonin (5-HT) and neuropeptide Y (NPY) in the brain of Scylla serrata, immunocytochemical method of Strept Avidin-Biotin-Complex was applied to observe the immunoreaction to two antibodies. Among all the 12 somal clusters and 11 neuropils of the brain in S.serrata, 5-HT-like immunoreactivity occurs in 4 somal clusters and 6 neuropils in the protocerebrum and deutocerebrum; while NPY-like immunoreactivity is present in 7 somal clusters and 3 neuropils in the deutocerebru...FundedbytheNationalNaturalScienceFoundationofChina(No.30300269

Histological Studies on Testes Development of the Mud Crab,Scylla serrata

【中文摘要】 组织学观察表明 ,锯缘青蟹 (Scyllaserrata)精巢发育周期可以分为精原细胞期、精母细胞期、精子细胞期、精子期和休止期 5个时期。在划分精巢发育期的基础上 ,结合生精小管直径和成熟节段比例这 2个指标 ,可以较准确地反映锯缘青蟹精巢的发育状况。而成熟节段比例可以作为锯缘青蟹精巢发育的表征指标。 【英文摘要】 The testicular development cycle of Scylla serrata can be divided into 5 periods:the spermatogonium stage,the spermatocyte stage,the spermatid stage,the sperm stage and the resting stage.Based on the division of the development cycle,the diameter of the seminiferous tubule and the ratio of its mature sections can reflect the process of the testicular development.For the first time,the ratio of the mature sections is presented as a characteristic index of the testicular development.福建省重中之重项

锯缘青蟹大颚器发育的组织学研究

采用组织学方法对锯缘青蟹 (Scyllaserrata )大颚器进行研究。大颚器位于大颚肌几丁质腱的后侧基部 ,发育过程可分为4期 :Ⅰ期大颚器很小 ,肉眼难以辨认 ,腺细胞数量少 ,细胞界限不清晰 ;Ⅱ期大颚器形状不确定 ,腺细胞开始形成腺泡 ;Ⅲ期大颚器通常呈肾形 ,腺泡多 ;Ⅳ期大颚器体积最大 ,呈柱状 ,腺细胞发达 ,未见退化现象。锯缘青蟹体长、卵母细胞直径、生精小管直径与大颚器腺细胞直径具有良好的线性关系。实验结果提示了锯缘青蟹大颚器与生长、生殖活动密切相关。 【英文摘要】 A pair of mandibular organs are located at the base of the mandibular tendon. Their development can be divided into 4 stages: in Stages Ⅰ, the glands are too small to identify with eyes, and the glandular cells are of small numbers. In Stage Ⅱ, the figure of the glands are not easy to be conformed, and the glandular cells begin to form gland alveoli. In Stage Ⅲ, the glands usually take on a kidney figure, and have many gland alveoli. In Stage Ⅳ, the highly vascularized glands are pole in shape, and the glan...福建省重中之重项目“福建省海洋生物优良种质和生物活性物质的应用基础研究”;; 高等学校博士学科点专项科研基金项目20010384010

Immunorecognition of FSH and LH in Optic Ganglion of Scylla serrata

【中文摘要】 甲壳动物的视神经节含有性腺抑制激素(Gonad inhibiting hormone,GIH),长期以来视神经节被理解为生殖抑制效应.本研究应用脊椎动物卵泡刺激素(Fllicle stimulating hormone,FSH)抗体和黄体生成素(Luteinizing hormone,LH)抗体,从锯缘青蟹(Scylla serrata)视神经节的视外髓、视内髓和视端髓检测到FSH和LH免疫细胞化学阳性反应.锯缘青蟹视神经节中的FSH和LH免疫阳性物质,可能参与了生殖神经内分泌调节. 【英文摘要】 In vertebrates,the gonadotropins are secreted by the adenohypophysis and are composed of the follicle stimulating hormone(FSH) and the luteinizing hormone(LH).These two hormones can combine with their receptors on the cytoplasmic membrane of gonadal cells,then stimulate the gonadal development and the production of the steroid hormones(estrogen and androgen).Thus,they have an important role in spermatogenesis and oogenesis.It is not yet reported whether FSH or LH exist in crustaceans.Optic ganglion is the s...国家自然科学基金(30300269)资

锯缘青蟹神经器官对卵巢发育的调节作用：离体实验

器官联合培养研究表明 ,锯缘青蟹(Scyllaserrata)的脑和胸神经团分泌的性腺刺激激素 (GSH)对卵母细胞的生长具有直接的促进作用 ,对视神经节则没有显著的影响。在卵巢发育过程中 ,脑和胸神经团GSH的生物活性逐渐增强。 【英文摘要】 In vitro study shows that the brain and the thoracic ganglion in female crabs are the source of gonad-stimulating hor-mone(GSH)which have direct stimulation upon the ovarian maturation,while the optical ganglion has no remarkable effect.The activities of GSH in the brain and the thoracic ganglion enhance during the ovarian development.福建省重中之重项目“福建省海洋生物优良种质和生物活性物质的应用基础研究” ;; 高等学校博士学科点专项科研基金项目20010384010号