The preparation of new collagen layer on PU sur face and the effect on the growth of endothelial cells

PU或PTFE 表面的內皮細胞化是降低 LVAD 導管血栓的主要方法之一。本研究 即以光化學方式將不同濃度 Gly-Arg-Gly-Asp(GRGD) peptide 接枝於 PU-PEG材料表面，並探討內皮細胞在材料 上貼附及成長情形。另外對PTFE 薄膜，我 們則以C2H2/N2 電漿處理，以期改善材料表 面，並試圖使內皮細胞成長於其上。至於 以collagen layer coating 在PU surface 上， 由於其它學者研究已成熟，不具研究價 值，因此只作適當的探討，其結果不列於 文中。 研究結果如下：以ATR-FTIR 測定經 改質的PU表面，發現有GRGD peptide 的 特性峰存在；經ESCA 分析，GRGD peptide 接枝濃度愈高，材料其N1S及O1S 比率也隨 之升高；上述資料顯示GRGD peptide 確實 已成功接枝於PU-PEG 表面上。內皮細胞 之培養，經MTT 測試，細胞的貼附率隨接 枝peptide 濃度升高而增加。同樣地，將四 聚氟乙烯(PTFE)薄膜利用電漿(C2H2/N2)作 表面處理，經由ESCA 分析，其N/C 比率 分別增加2 倍以上，且F/C 比率亦減少了6 倍以上(data not show)，由此顯示PTFE 薄 膜表面成功地被電漿披覆。經內皮細胞培 養後，以MTT 測試發現經電漿改質的材料 表面確實可促進內皮細胞貼附生長。Surface endothelization of PU or PTFE is an important way to reduce thrombosis and enhance its blood compatibility. This project tried to photochemically graft GRGD peptide to PU-PEG surface and plasma treat PTFE membrane with C2H2/N2 for endothelization of those materials. The results slowed that GRGD peptide were successfully grafted to PU-PEG surface confirmed by analysis of ATR-FTIR and ESCA. Moreover, MTT tests for cell metabolism showed that the higher the peptide concentration grafted, the more the cells adhered and proliferated on the matrix. In regards to plasma treatment on PTFE membrane, surface N/C and F/C ratios were two times increases and six times decreases respectively, that indicated successful the modifications of PTFE surface. Moreover, post-treatment PTFE membrane could adhere and proliferate endothelial cells confirmed by photograph and MTT tests while nontreatment one was not good for cell adhesion. In conclusion, surface modification with peptide for PU-PEG film and C2H2/N2 plasma treatment for PTFE membrane could enhance endothelial cells adhesion and proliferation