Immunohistochemical Study of Serotonin in the Digestive System of Scylla serrata

【中文摘要】 应用免疫组织化学方法对锯缘青蟹消化系统的 5- HT分泌细胞的形态和分布进行了观察 .结果表明 :5- HT阳性细胞形态多样 ,多数为卵圆形 ,少数为柱形 ,多边形或具有较长的胞突 .消化道各段均有 5- HT阳性细胞 ,中肠密度最高 ,食道次之 ,贲门胃、幽门胃和后肠较低 .5- HT阳性细胞多数位于固有膜和粘膜下层 ,少数散布于肌层 .肝胰腺也具有 5- HT阳性细胞 【英文摘要】 The morphology and distribution of serotonin(5 HT) immunoreactivity in the digestive system of Scylla serrata were investigated. 5 HT immunoreactive cells had various forms: the majority were oval, while the minority were columnar, irregular in shape, or having long processes. 5 HT immunoreactive cells were distributed throughout the digestive tract, with the highest density in the midgut, the second in the esophagus, and a low density in the stomach and hindgut. Most of the 5 HT immunoreactive c...福建省重中之重“福建省海洋生物优良种质和生物活性物质的应用基础研究”资助项

尼罗罗非鱼TCP-1-beta和TCP-1-eta的分子特征及其低温诱导表达

为了研究尼罗罗非鱼耐寒性状的分子基础并为耐寒品种选育提供参考,研究从尼罗罗非鱼中克隆了HSP60家族TCP-1-beta和TCP-1-eta基因并对其在低温诱导下的表达特征进行了分析。尼罗罗非鱼TCP-1-beta cDNA长度为1755 bp,包括1605 bp的完整开放阅读框,编码534个氨基酸;尼罗罗非鱼TCP-1-eta cDNA长度1651 bp,包括1638 bp的完整开放阅读框,编码545个氨基酸。与其他物种同源基因的蛋白序列比对结果显示,TCP-1-beta和TCP-1-eta蛋白在物种间同源性很高,且都具有保守的ATP结合结构域等,预示其在物种间功能的保守性。实时荧光定量PCR结果表明:TCP-1-beta和TCP-1-eta在各组织中呈遍在表达,但在肌肉中表达量最高;诱导温度从22℃降至12℃,不同低温诱导48h后TCP-1-beta和TCP-1-eta均呈上调表达,在18℃时表达开始上调,随着低温胁迫程度加强,表达上调幅度增大,至12℃时表达量达到最高,TCP-1-beta和TCP-1-eta上调幅度分别达到常温的12.2倍和10.7倍。这些结果预示在尼罗罗非鱼中,TCP-1-beta和TCP-1-eta是潜在的耐寒相关基因

Immunorecognition of Estrogen Receptor in the Optic Ganglion of Scylla serrata

【中文摘要】 利用免疫细胞化学SABC法对锯缘青蟹(Scylla serrata)视神经节雌激素受体(ER)进行定位研究.结果表明:视神经节的视外髓、视内髓和视端髓存在ER免疫阳性细胞.视神经节存在的ER免疫阳性细胞可以为雌激素提供结合位点.本研究为雌激素参与视神经节神经内分泌调节作用提供了形态学依据. 【英文摘要】 Antisera generated in vertebrate and immunocytochemical SABC method was applied to observe the immunoreactive neurons and neuropils of estrogen receptor(ER) in optic ganglia of Scylla serrata.The results showed that:ER-immunoreactive substance was located in medulla externa,medulla interna and medulla terminalis in optic ganglion.ER-immunoreactive neurons in optic ganglia might provide the binding sites for estrogen and accept the feedback regulation of estrogen.This present work provides the morphological ...国家自然科学基金(30300269)资

KARYOLOGICAL STUDIES OF THE HYBRID LARVAE OF HALIOTIS DISVERSICOLOR SUPERTEXTA FEMALE AND HALIOTIS DISCUS DISCUS MALE

To determine the genomic composition of the interspecific hybrid between Haliotis diversicolor supertexta female and H. discus discus male at an early developmental stage, veliger larvae produced from hybrid (SJ-5 and SJ-50) and pure species crosses (SS and JJ) were sampled and analyzed using standard karyological methods and genomic in situ hybridization. In hybrid metaphase spreads, chromosomes from both parents were detected, except one metaphase, which showed the H. diversicolor supertexta haploid karyotype. The genomic composition of the hybrid was also confirmed through preliminary genomic in situ hybridization results. Many more aneuploids and chromosome fragments were found in the hybrids than those in the control pure species crosses, indicating genome instability and chromosome loss in the hybrids. In the hybrid hypodiploid metaphase spreads, two intact sets of H. diversicolor supertexta chromosomes and several H. discus discus chromosomes were detected by pairing. Spontaneous diploidization of the maternal chromosome set was shown to occur in hybrid larvae, as 2.2% heterogeneous triploid and 17.9% hypodiploids with two intact H. diversicolor supertexta chromosome sets for SJ-5. The current findings suggest that uniparental chromosome elimination along with spontaneous diploidization of maternal chromosome sets may be the reason for allogynogenesis production in H. diversicolor supertexta X H. discus discus hybridization

Status of Mud Crabs(Scylla spp.) Farming and Studies on the Population Biology of S. paramamosain

综述了国内外青蟹养殖的基本情况,包括养殖模式、养殖历史、人工育苗的现状,我国青蟹养殖在海水养殖中的地位及其特点,以及我国拟穴青蟹种群生物学研究现状及其在养殖中的重要性.This paper summarized the internationally general situation of mud crabs( Scylla spp.) farming,including cultivation pattern,cultural history,the situation of artificial mass larvae culturing,study progresses of population biology of S. paramamosain and its significance in its farming in China,as well as the status and characteristics of China mud crab farming.国家自然科学基金(31472294

Influence of N, P additions on the transfer of nickel along the food-chain

【中文摘要】 采用放射性同位素示踪法,研究氮或磷添加对镍在东海原甲藻(Prorocentrum donghaienseLu)和中肋骨条藻(Skeletonema costatum)胞内累积的影响;并将其分别投喂中华哲水蚤(Calanus sinicus)后,探讨镍在桡足类体内的吸收和生理周转。结果表明,经过24h暴露后,氮或磷显著影响这两种藻类对镍的吸收,且高浓度氮或磷均能促进镍在这两种藻类细胞内的累积,因而促进镍在中华哲水蚤体内的吸收;而镍在中华哲水蚤体内的生理周转率却不受氮或磷浓度的影响,似乎更受动物个体的生理状态影响;中华哲水蚤对镍的吸收率与藻类细胞内镍含量呈极显著相关(p<0.001),证明了"桡足类只吸收饵料细胞内的水相金属库"的假说。由此可见,近海富营养化可促进浮游植物对镍的吸收,进而影响该金属在浮游生物食物链上的传递。 【英文摘要】 We examined the influence of macronutrient (nitrate or phosphate) additions on the bioaccumulation of Ni in two species of phytoplankton (Prorocentrum donghaiense Lu and Skeletonema costatum), and its subsequent assimilation and physiological turnover in marine copepod (Calanus sinicus) after ingestion, using a radioactive-tracer method. The results showed that N or P addition significantly influenced intracellular Ni accumulation in the algal cells after 24 h of exposure, with higher ambient N or P enhanci...国家自然科学基金委员会与香港研究资助局联合科研资助基金(40131160735);国家自然科学基金资助项目(40576065

拟穴青蟹受精膜形成过程中环形颗粒的发生与胞吐

为了揭示拟穴青蟹受精膜形成机制,采用透射电子显微镜研究了其受精膜形成过程中环形颗粒的形成与胞吐。结果显示,拟穴青蟹精子入卵后,发生皮层反应而形成受精膜。皮层反应包括致密颗粒首先胞吐以及环形颗粒的相继多轮胞吐。每轮环形颗粒的胞吐参与到受精膜的平缓期、增厚期和举起期3个阶段。环形颗粒的形成主要发生在受精膜的增厚期,受精卵的皮层和靠近皮层的内质中都在形成大量的环形颗粒,并且迅速移到皮层质膜附近发生爆发性胞吐,参与到受精膜的快速形成。环形颗粒的形成与2种卵黄颗粒以及脂滴密切相关,在皮层中发现了线粒体参与其中,推测在拟穴青蟹受精卵的皮层和内质可能存在环形颗粒合成的两套不同机制。本文证实了卵黄颗粒和脂滴及环形颗粒在拟穴青蟹受精膜形成过程中发挥重要作用。国家“八六三”高技术研究发展计划(2002AA603013);;福建省教育厅科技项目(JAT160256);;集美大学学科建设基金(C517064

Study on spermatophore storage and dehiscence in crab

【中文摘要】 甲壳动物在海洋中分布广泛,有多种精子传输方式,大部分产生精荚,粘于雌体腹部或通过交接器输送到纳精囊中[1]。研究表明,在蟹类交配、精子转移、贮存和受精等过程中,存在如精子竞争和亲体保护,多次受精等众多复杂事件[2-4]。这些过程主要是以蟹类纳精囊为核心展开,为此本文综述了纳精囊的结构和功能,精子活力的维持、精荚形态、精子竞争和转移等近年研究进展,旨为相关研究提供参考。1精荚的接受和贮存1.1纳精囊的一般结构短尾类精子贮存结构比较多样。通常蟹类在交配时获得雄蟹排出的精液,在雌蟹受精囊(sem inal receptacyles)即纳精囊(spermatheca)中贮存很长时间。蟹类的纳精囊结构都很相似,一般可分为4个区,分别为输卵管、纳精囊囊部、阴道及阴户(图1[5],图2[6])。许多蟹类的纳精囊已有过研究[7-24]。纳精囊是输卵管和卵巢相联部位前段的膨大,阴道开在纳精囊基部,开口在卵孔处。纳精囊由前端腺质层和后端几丁质区组成,其后端与具几丁质内膜的阴道相联。未交配时,纳精囊很薄、呈现白色、经度方向为透明的扁平囊[10]。前端腺体层由两层组成,最外层为很薄的上皮,呈现膜状,由有核的上皮细胞组成,内上皮很厚,... 【英文摘要】 In general,crab receives copious quantities of seminal fluid during mating,and stores them in special seminal receptacles,viz.spermatheca,for a prolonged period.The spermatheca of the advanced brachyuran crabs has been described for many species.The spermatheca is a dilation of the proximal region of the oviduct connecting it to the ovary.The vagina leads from the base of the spermatheca and opens outside the ovipore.Some crabs have the special structure,such as valve-like tissue,bursa,intermediate chamber....国家“八六三”资助项目(2002AA603013

现代生物技术在水产动物健康养殖中的应用

现代生物技术的应用对水产动物健康养殖产生了很大的影响 .本文从水产动物疾病诊断 ,高效疫苗制备 ,抗病品种培育 ,提高抗病力 ,水体污染监测等方面对现代生物技术的应用进行了综述 .旨在为水产动物健康养殖及相关研究提供参考 【英文摘要】 More and more modern biotechnologies were applied extensively to aquaculture with development of molecular biology, thus great impacts were produced in the process of culture and husbandry, especially, on the healthy managements of aquatic animal culture. It was summarized for application of modern biotechnologies from diagnostication of aquatic animal diseases, preparation of high effect vaccine, breeding of anti disease strain, improvement of anti disease capacity and inspection of aquatic pollution, wi...国家 8 6 3重大专项 (2 0 0 2 AA6 0 30 1 3

Changes of fatty acid composition in muscle and muscle cell membrane of Scylla serrata under low temperature adaptation

【中文摘要】 研究主要采用生物化学方法对低温适应3周的锯缘青蟹肌肉及肌细胞膜脂肪酸组成进行测定,结果显示,肌肉中脂肪酸C16:0随饲养温度降低而降低,5℃组显著低于27℃组(P<0.05);C18:0却在5℃组显著高于27℃组(P<0.05);C20:5随着饲养温度降低而升高,5℃和10℃组显著高于27℃组(P<0.05)。∑C16随饲养温度的降低而降低,5℃组显著低于27℃组(P<0.01);∑C20却随饲养温度的降低而升高,5℃和10℃组显著高于27℃组(P<0.05);EPA+DHA随饲养温度降低而升高,5℃和10℃组显著高于27℃组(P<0.05),而EPA/DHA仅5℃组显著高于27℃组(P<0.01)。低温适应下锯缘青蟹肌肉细胞膜脂肪酸C18:2显著升高(P<0.01或P<0.05),但随养殖温度的降低逐渐降低;低温适应下C18:1降低,在5℃组显著降低(P<0.05);C18:0低温适应下也显著降低(P<0.01);C20:5低温适应下上升,但仅在15℃适应下显著上升(P<0.05)。C22:6低温环境下降低,在5℃和10℃组显著下降(P<0.05)。C20:1,C20:4和C20:3在低温环境下增加,仅5℃... 【英文摘要】 Mud crab,Scylla serrata,is an important commercial crustacean living in estuarine waters.Studies on fatty acids composition changes in muscle and cell membrane of S.serrata under low temperature adaptation play an important role in understanding physiological adaptation at low temperature.In this study,S.serrata were forced to experience 3-week adaptation at different low temperatures.Biochemistry methods were used to determine the content of various fatty acids in muscle as well as cell membrane.For muscle...国家“863”重大专项(2002AA603013