戊型肝炎病毒衣壳蛋白特异性人源单克隆抗体的筛选与鉴定

目的:建立从外周血快速筛选戊型肝炎病毒(Hepatitis E virus,HEV)衣壳蛋白特异性人源抗体的方法,从疫苗免疫者外周血中筛选出相应抗体并进行鉴定。方法:采用分选型流式细胞仪获得外周血中HEV衣壳蛋白特异性的记忆B细胞,通过单细胞RT-PCR的方法获得抗体序列,并进行重组表达,最后对获得的人源单克隆抗体进行初步性质鉴定。结果:成功筛选到识别HEV衣壳蛋白的6株人源单克隆抗体,6株抗体均具有抗原结合活性,4株抗体具有中和活性。结论:成功获得HEV衣壳蛋白特异性人源单克隆抗体序列,并进行真核表达,对抗体的性质进行初步鉴定,为后期研究疫苗免疫的人体内的抗体演化打下基础。国家自然科学基金(No.81571996);;福建省自然科学基金(No.2016J05201)资

SOLAR AIR CONDITIONING UTILIZING ORGANIC RANKINE-VAPOR COMPRESSION SYSTEM

To develop the organic Rankine-vapor compression air conditioning driven by solar, a thermodynamic model was developed, and the working fluid type, system design and influencing factors were researched. The results showed that the generation temperature

太阳能驱动的有机朗肯蒸汽压缩空调

To develop the organic Rankine-vapor compression air conditioning driven by solar, a thermodynamic model was developed, and the working fluid type, system design and influencing factors were researched. The results showed that the generation temperature

东北北部冻土退化与寒区生态环境变化

我国东北地区位于中高纬度欧亚大陆东缘,大、小兴安岭多年冻土区是欧亚大陆高纬冻土区向南最突出的部位,属于高纬山地冻土.东北多年冻土区是我国,乃至全球范围内,受气候变暖和人为活动影响最显著的冻土区之一.过去40 a来该区冻土显著退化,主要表现在:1)冻土南界及不连续多年冻土各分区边界北移而导致总面积减小、空间分布破碎化;2)活动层加深,融区扩大,局地冻土岛消失;3)冻土温度升高、厚度减薄、热稳定性降低等.由于各种因素的共同影响,寒区生态环境也发生了一系列变化.这具体表现为以兴安落叶松占绝对优势的天然林带锐减,整个北方森林带北移,沼泽湿地面积减小等,寒区生态系统和环境已出现恶性循环.关注、研究、整治和管护寒区环境对区域社会、经济和生态可持续发展不可或缺

The Establishment of High-throughput Neutralization Titer Evaluation Model for Hepatitis E Virus(HEV)

广泛有效的体外感染模型的缺乏限制了抗戊型肝炎病毒(HEPATITIS E VIruS,HEV)抗体及血清定量化中和效价的评估,从而阻碍了对HEV相关抗体应答及免疫机制的深入研究。本研究首先通过在HEV低效复制细胞系HEPg2肝癌细胞株上进行了HEV感染细胞后的连续监测,直到第13d到达病毒载量的检测下限,验证了该细胞系建立感染模型的可行性,进一步采用96孔多通道平行感染、核酸提取及实时荧光定量PCr对五株抗HEV的鼠源单克隆抗体及四位戊肝疫苗接种者的接种前、后血清样本进行了细胞中和试验。结果显示应用该模型能够实现对不同中和能力的单抗及接种疫苗前后的血清进行中和效价的定量化评估。表明本研究已成功建立了HEV体外高通量中和评价模型,同时也显示出该模型在HEV疫苗及抗体应答表位研究中所具有的潜在价值。The lack of effective in vitroinfection model for hepatitis E virus(HEV)has greatly hindered the quantitative analysis of neutralizing titers of anti-HEV antibodies and human sera,thus impeding further studies of HEV-stimulated antibody responses and the immunological mechanisms.In order to improve this situation,the infection of HepG2 cells that are inefficient for HEV replication was continuously monitored until the viral load reached the limit of detection on day 13,the results of which confirmed the feasibility of using this cell line to establish the infection model.Then,neutralization assays of five anti-HEV murine monoclonal antibodies and serum samples collected from four HEV vaccine recipients(collected before and after vaccination)were performed by 96multi-channel parallel infections,nucleic acid extraction,and qPCR.The results showed that the cell model can be applied for quantitative evaluation of the neutralizing capacity of different antibodies and antiserum samples from HEV vaccine recipients.In this study,we have successfully established a high-throughput in vitro HEV replication model,which will prove to be useful for the evaluation of HEV vaccines and studies of HEV epitopes.国家基金(81373061); 重大新药创制(2013ZX09101017); 厦门市科技计划项目(3502Z20131001

TiN coating for inner surface modification by grid enhanced plasma source ion implantation

Using the new inner surface modification technique named GEPS II (grid-enhanced plasma source ion implantation), which is designed for inner surface modification of tubular work piece, has successfully produced TiN coating on 0.45% C steel (45 # steel). By comparing with the uncoated 45 # steel sample, the electrochemical corrosion test on the coated 45 # steel samples presents about 5-10 times improvement in their corrosion resistance. Structural analysis shows that the TiN coatings have preferred crystal orientation(111) and (2000). And the depth of coatings is about 20 nm, with part of which embedding in the substrate