Job Consumption: Compensation or Benefit Expropriation

在职消费具有薪酬补偿与利益侵占的双刃剑效应,其对企业绩效的影响方向及程度是这两股作用力的博弈结果,而这两种效应的发挥又受到管理层激励与约束程度的影响。为探索在职消费的业绩影响,分别以显性激励水平和管理层权利为标准分情境讨论了在职消费的双刃剑效应,研究发现:在管理层显性激励水平相对越低的情境下,在职消费越能有效提高企业绩效,在职消费的薪酬补偿效应越显著;在管理层约束程度越弱的情境下,在职消费对企业绩效产生负向影响,在职消费的利益侵占效应越显著;与民企相比,国企在职消费所发挥的薪酬补偿效应更大。Perks have double - edged sword effect of compensation and benefit expropriation, the direction and degree of its influence on enterprise performance depends on the game result of these two forces, and the two types of effects＇ play are affected by the explicit incentive level of management and management rights. Based on explicit incentive level and management rights norm, this paper discussed the double -edged effect of perks in different situations, and find that： （~）At the situation of the explicit incentive level is lower, perks can effectively improve business performance and the compensation effect is more significant. ①At the situation of the management rights is stronger, perks reduces the corporate performance and the benefit expropriation effect is more significant. ②Compared to private enterprise, perks of state - owned enterprises have bigger compensation effect.北京林业大学研究生课程建设项目(HXKC15028)；中央高校基本科研业务费专项资金资助项目“基于云模型的高管声誉评价体系构建及薪酬结构分层设计”(RW2015-21)

Molecular Cloning and Its Transformation of ACC Synthase NtACS1Gene from Narcissus tazetta var.Yunxiang'

为探究多花水仙ACS基因的序列特征及功能,以云香'水仙盛花期花瓣为试验材料,根据云香'水仙花朵转录组数据信息,通过RT-PCR方法克隆出1个AC; S基因,命名为NtACS1(GenBank; KX082936);NtACS1开放阅读框(ORF)长度为552bp,编码183个氨基酸。编码蛋白质分子量约为20.6KDa,理论等电点为6.3; 0,不稳定系数为65.49,属于不稳定的疏水性蛋白。通过qRT-PCR对云香'水仙不同时期花瓣和副冠中的NtACS1基因进行了表达分析,得到与云; 香'水仙花朵转录组数据中相同的结果:NtACS1基因在云香'水仙花瓣和副冠中的表达都是随着花衰老过程呈现逐渐下降的趋势,且NtACS1基因在花瓣; 和副冠中的表达峰值都在花苞期,表明; NtACS1基因编码的蛋白是在乙烯生物合成途径的系统1发挥催化作用的ACC合成酶。成功构建了NtACS1基因的正义植物表达载体,并通过农杆菌介导; 法获得8株转基因烟草,PCR和RT-PCR检测显示其中有6株为阳性植株,初步证实NtACS1基因已导入烟草基因组中且在烟草中已表达。该研究结果为; 进一步分析NtACS1基因的功能和后续转化水仙延长其花期研究奠定了基础。Aimed to study the characteristics and functions of ACSgene,in the; present study,we cloned a 1-aminocyclopropane-1-carboxylate synthetase; gene named NtACS1(GeneBank KX082936)based on the RNA-Seq database from; the flower of Narcissus tazetta var.Yunxiang'using RT-PCR method.The; length of the open reading frame(ORF)of ACSis 552bp,encoding 183amino; acids coupled with a molecular weight of 20.6kDa and theoretical; isoelectric point of 6.30.qRT-PCR analysis showed that the relative; expression level of NtACS1both in petals and coronas are decreased; gradually along with the aging of flower.Moreover,the expression data of; NtACS1gene were consistent with those obtained by RNA-Seq, implied that; the NtACS1protein as an ACC synthetase might play a role in the; catalytic system 1 of ethylene biosynthesis.Furthermore,sense plant; expression vectors of NtACS1were successfully constructed with; agrobacterium mediated transformation,and 6positive transgenic tobacco; plants were ultimately obtained. Our current study will lay an; experimental foundation for the future application of the genetic; transformation to prolong florescence ofYunxiang'.福建省种业创新与产业化工程项

Isolation and Function Analysis of NtWRKYY1Transcription Factor Gene in Narcissus tazetea var.Yunxiang

为明确WRKY转录因子在云香水仙中的功能,该研究以云香水仙为材料,克隆了WRKY基因,命名为NtWRKYY1(GenBank登录号为KX0564; 95)。序列分析显示,NtWRKYY1基因开放阅读框(ORF)长度为510; bp,编码169个氨基酸。多序列比对和系统进化树分析显示,NtWRKYY1编码蛋白含1个WRKY结构域和C_2H_2锌指结构(Cx_4Cx_(2; 3)HxH),与AtWRKY57聚在一起,属于第Ⅱc类WRKY转录因子。组织表达和时空表达分析显示,NtWRKYY1基因在花中表达量高于根和叶,; 且在花瓣及副冠中的表达量随开花过程(花蕾期、始花期、盛花期、衰败期)呈上升趋势。植物激素和非生物胁迫分析显示,NtWRKYY1基因受脱落酸(AB; A)、高温、干旱和盐诱导,受茉莉酸甲酯(JA)抑制,表明NtWRKYY1基因可能在云香水仙花朵的衰老过程中起正调节作用,同时参与云香水仙ABA、; JA等激素信号转导及高温、干旱、盐碱等非生物胁迫过程的调控。利用In-Fusion克隆技术成功构建过表达载体pMDC140-NtWRKYY1,并; 采用农杆菌介导叶盘法转化烟草。RT-PCR和GUS染色结果显示,目的基因已成功导入烟草基因组中。WRKY transcription factors play an important adjusting role in the; process of plant growth and development,hormone signal transduction and; abiotic stress response.To clarify the gene Narcissus tazetea; var.Yunxiang,we cloned the NtWRKYY1gene(GenBank accession; No.KX056495)based on Yunxiang,analyzed the gene sequence; features,evolutionary relationship and expression characteristics,; constructed the expression vector before transformed into; tobacco.Sequence analysis revealed that the length of NtWRKYY1gene open; reading frame(ORF)is 510bp,encoding apolypeptide of 169amino; acids.Multiple alignments and phylogenetic analysis showed that; NtWRKYY1protein containing one WRKY consecutive structural domain and; C_2H_2type zinc finger(Cx_4Cx_(23)HxH)belong toⅡc sub-group of WRKY; transcription factor together with Arabidopsis AtWRKY57.Tissue-specific; expression and temporal and spatial expression showed that; NtWRKYY1inYunxianghad a much higher expression in flowers than that in; roots and leaves,and a rising express trend in petal and corona of; alabastrum stage,early flowering stage,full bloom stage and faded; stage.NtWRKYY1can be induced by abscisic acid(ABA), high; temperature,drought and saline,and restrained by methyl; jasmonate(JA)through hormones and abiotic stresses analyzing.We can; concluded that NtWRKYY1gene may play a regulating role during the flower; senescence process inYunxiang,involve in the hormone signal transduction; of ABA,JA and the abiotic stress regulation of high temperature,drought; and saline at the same time.In addition,we constructed overexpressing; vector pMDC140-NtWRKYY1using In-Fusion cloning technique,transformed; into tobacco by the method of Agrobacteriumthough leaf disc; transformation.The carrier of PCR and GUS staining results indicated the; resistant plantlets were positive.This study will make a good foundation; for further exploring the function of the WRKY transcription factor; inYunxiang.福建省种业创新与产业化工程项