Effect of Ethanol Extract from Camellia Oleifera Abel. on Blood Glucose in Type 2 Diabetic Mice

目的筛选油茶（Camellia oleifera Abel.）降低2型糖尿病小鼠血糖的有效部位。方法采用腹腔注射烟碱、链脲霉素诱导的2型糖尿病小鼠模型,应用超声提取法分别获得油茶叶、果、籽的乙醇提取物,筛选降低血糖的有效部位。结果阳性药罗格列酮每日灌胃剂量10mg/kg·BW,连续灌胃21d后,降低了2型糖尿病小鼠血糖。分别灌胃油茶叶、油茶果、油茶籽乙醇提取物,按每日剂量100mg/kg·BW连续灌胃21d后,均在一定程度上降低2型糖尿病小鼠血糖,油茶籽乙醇提取物对口服葡萄糖耐受试验（Oral glucose tolerance test,OGTT）作用最明显。与对照组相比,1μM和2μM的阳性药罗格列酮均能明显促进3T3-L1前脂肪细胞分化;油茶籽乙醇提取物在0.5μg·m L-1和1μg·m L-1浓度下也体现出促进分化的活性。结论油茶籽乙醇提取物降低了2型糖尿病小鼠的血糖,促进了3T3-L1前脂肪细胞的分化,但其具体作用机制尚需深入研究。OBJECTIVE To investigate effective parts possessing property of lowering blood glucose in type 2diabetic mice from Camellia oleifera Abel. METHODS Leaf,fruit and seed ethanol extract of C. oleifera were obtained through ultrasonic extraction,and were subsequently subject to type 2 diabetic mice model induced by intraperitoneal injection of streptozocin and nicotine. RESULTS In accordance with the positive drug rosiglitazone by intragastric administration once daily dose of 10 mg / kg·BW,continuous intragastric administration for 21 days,blood glucose in type 2 diabetic mice was reduced. In accordance with a daily dose of 100 mg / kg·BW leaf,fruit and seed ethanol extract of C. oleifera,continuous intragastric administration for 21 days respectively,in a certain extent,blood glucose in type 2 diabetic mice was reduced. Moreover,effect of seed ethanol extract on blood glucose and oral glucose tolerance test（ OGTT） is the most obvious. Compared with control group（ treated with insulin）,1μM and 2μM of positive drug rosiglitazone can significantly promote differentiation of 3T3-L1 pre-adipocytes,C. oleifera seed ethanol extract as the most effective part for lowing blood glucose at 0. 5μg·m L~（-1） and 1μg·m L~（-1）concentration also promoted 3T3-L1 pre-adipocyte differentiation. CONCLUSION It was suggested that C. oleifera seed ethanol extract lowered blood glucose in type 2 diabetic mice and enhanced differentiation of 3T3-L1 pre-adipocytes,but the specific mechanism needs further study.福建省教育厅中青年教师教育科研项目杰青项目JA14421;福建省教育厅中青年教师教育科研项目JA13425; 厦门市科技局科技计划高校创新项目3502Z20143026;厦门市科技局科技计划项目3502Z20144031; 福建省自然科学基金计划资助项目2015J01065; 福建省科技厅自然科学基金青年创新项目2014D008; 福建省卫生系统中青年骨干人才培养项目基础项目2015-ZQN-JC-45; 厦门市卫生和计划生育委员会资助研究经费; 厦门医学高等专科学校科研基金Z2013-12;Z2013-2

在天然药物化学实训中培养学生创新能力

为满足社会对创新型药学高级人才的迫切需求,高职教育需要加强培养学生的创新能力。天然药物化学是药学、药物制剂等专业的一门非常重要的专业课和专业基础课。基于天然药物化学实践性强的特点,通过天然药物化学实训教学培养学生综合专业素质和创新思维。福建省自然科学基金青年创新项目2014年资助课题2014D008; 福建省卫生系统中青年骨干人才培养项目2015年资助计划基础项目2015-ZQN-JC-45; 厦门市卫生和计划生育委员会资助研究经费; 福建省中青年教师教育科研项目2014年资助课题杰青项目JA14421;福建省中青年教师教育科研项目2013年资助课题A13425; 厦门市科技计划高校创新项目2014年资助课题3502Z20143026; 厦门医学高等专科学校教学研究类项目2014年资助课题J2014-07;厦门医学高等专科学