The contents and dynamics of different forms of phosphorus in shrimp culture area of Pantu, Tong'an Bay, Xiamen

１９９６年５至１９９７年２月对厦门市同安湾潘涂垦区两口虾池及进水渠道不同形态磷的含量与动态进行了 调查。结果显示，虾池的颗粒磷（ＰＰ）含量明显高于邻近内湾，可溶性有机磷（ＤＯＰ）含量略高于内湾，可溶性无 机磷酸盐（ＤＩＰ）含量则明显比邻近内湾的低。虾池中的磷主要以ＰＰ形态存在（７８．２５％），ＤＯＰ、ＤＩＰ含 量较少（ｌ．８２％和６．９３％）；垦区内湾则是以ＰＰ和ＤＩＰ为主（４４．４９％和４６，３９％），ＤＯＰ所占比例最小 （９．１２％）。虾池与垦区内湾不同形态磷的季节变化也存在一定差异。From May 1996 to Feb. 1997, the contents and dynamics of different forms of phosphorus in two shrimp ponds and the inflow channel in Pantu cultivation area in Tong' an Bay, Xiamen were surveyed. The results showed that the content of particle phosphorus (PP) in shrimp ponds was significantly higher than that in the neighboring bay, while the content of dissolved organic phosphorus (DOP) in shrimp ponds was slightly higher, and the content of dissolved inorganic phosphorus (DIP) was evidently lower than that in the neighboring bay. PP was the main existing form of phosphorus in shrimp ponds (78 ..25 %), the contents of DOP and DIP were lower (14.82% and 6.93%, respectively), while in the neighboring bay phosphorus exists mostly in the forms of PP and DIP (44.49% and 46.39%, respectively), and DOP only constitutes 9.12%. The seasonal variations of the different forms of phosphorus in shrimp ponds are different from those in the neighboring bay.厦门市重大科技项目招标课题资助（1993－21－1－1

Over-expression of Pygo2 Promotes C6 Cells Proliferation of Glioma

目的通过构建过表达PygO2的重组体上调PygO2表达,探讨其在大鼠胶质瘤C6细胞增殖中的作用及机制。方法重组体经ECOr I和HIndⅢ双酶切鉴定和dnA测序后,用脂质体2000将其转染大鼠胶质瘤C6细胞,采用WESTErn blOT检测外源PygO2蛋白表达,应用克隆形成实验和MTT法检测细胞增殖,流式细胞术检测细胞周期,采用WESTErn blOT检测过表达PygO2对C6细胞中CyClInd1、β-CATEnIn水平的影响,并采用细胞免疫荧光法检测其对C6细胞中CyClInd1、β-CATEnIn亚细胞定位的影响。结果双酶切和测序鉴定结果证实插入序列正确,重组体能有效上调PygO2表达。将重组体转染C6细胞上调PygO2表达后,细胞的生长增殖被显著促进,克隆形成显著增多,细胞周期进程从g1期至S期转变显著增强;且CyClInd1水平随之增高,亚定位无改变,β-CATEnIn水平和亚细胞定位无明显改变。结论成功构建了过表达PygO2的重组体,过表达PygO2通过增高CyClInd1水平,促进细胞从g1期进入S期,从而促进大鼠胶质瘤C6细胞增殖。Objective To up-regulate expression of Pygopus2(Pygo2) by construction of the recombinant vectors of over-expression of Pygo2 protein,and to explore the role and mechanism of over-expression of Pygo2 in C6 cells proliferation of glioma.Methods The recombinant plasmids were digested with EcoRⅠ and Hind Ⅲ to execute the restriction endonuclease identification,then the sequence analysis was assayed by DNA sequencing.The recombinant plasmids were transfected into cultured glioblastoma C6 cells using lipofectamineTM 2000.The exogenous Pygo2 protein level of C6 cells was detected by Western blot analysis.Colony forming assay and MTT assay were used to detect the cell proliferation,and cell cycle analysis was performed by flow cytometry analysis.The effect of Pygo2 over-expression on the level of cyclinD1 and β-catenin of C6 cells was detected by Western blot analysis,and the expression and subcellular location of cyclinD1 and β-catenin of C6 cells were further quantified by immunofluorescent staining.Results The recombinant plasmids were completely coincided with the designs by the restriction map and the sequence analysis,which up-regulated Pygo2 expression of C6 cells efficiently.After Pygo2 expression were up-regulated by transfected C6 cells with the recombinant plasmids,cells proliferation was promoted and colony forming was increased significantly,cell cycle progression from G1 to S transition was enhanced notably.Furthermore,the expression level of cyclinD1 was significantly increased without change of subcellular location,and the expression level and subcellular location of β-catenin were not changed obviously.Conclusion The recombinant vectors of Pygo2 over-expression were constructed successfully.Over-expression of Pygo2 promotes the growth of glioma cells by an increased expression of cyclinD1 to improve G1/S transition.重庆市自然科学基金资助项目(cstc2011jjA10110);重庆市教委科技基金资助项目(KJ100504);福建省自然科学基金资助项目(2009D002

过渡领域圆柱间Couette流动

圆柱间Couette流动问题是最简单的流动情况之一,当圆柱转速较大(M≈1)而间隙与半径之比[δ=(R_2-R_1)/R_1]不为小量时,却可用以检验求解过渡领域中非线性问题的各种方法。不久前,文献[1,2]在M≈1,δ=0.5条件下用电子束做了同心圆柱间氩气密度分布的测量,提供了一组比较流场结构的实验数据。在较早的稀薄气体Couett

Studies on Uptake kinetics of Soluble Reactive Phosphorus(SRP) by Microplankton in Xiamen Harbor

于1990年4个季节在厦门港进行对浮游生物（＜200μM）对SrP吸收动力学的现场实验.结果表明，SrP吸收动力学4个季节都符合MICHAElIS-MEnTEn方程，最大吸收速率（VMAX）是夏＞春＞秋＞冬，分别是各季SrP吸收速率的1.10，2.33，3.09和3.55倍；SrP吸收半饱和常数（kS）是夏＜秋＜冬＜春，分别是各季SrP的1.29，2.30，2.19和3.69倍，与SrP成负相关.初步阐明了自然浮游生物群落在不同季节对SrP的吸收特性和生态适应机制；首次探讨了运用32PO3-4同位素稀释技术研究营养盐吸收动力学的实验方法.SRP uptake kinetics by microplankton experiments in situ were conducted seasonally from Feb.to Nov.1990 in Xiamen Harbor.Results showed that SRP uptake kinetics in all seasons could fit MichaelisMenten equation and seasonal variations in the parameters of SRP uptake kinetics,maximum uptake rates (V_max, summer>spring>fall>winter) and half uptake saturation constants (Ks,summer<fall<winter<spring).V_max was negatively correlated with SRP.V_max was 1.10, 2.33, 3.09, 3.55 times to SRP uptake rates and Ks was 1.29, 3.69, 2.30, 2.19 times to SRP in summer,spring,fall and winter respectively.Differences of SRP uptake kinetics were reflections of ecological adaptive mechanisms of natural microplankton assemblages in different seasons.Experimental methods for nutrient uptake kinitics by using 32PO_4--3- isotope dilution techniques were firstly inquired into in the paper

Studies on the Sexual Reproduction of the Marine Diatem Biddulphia sinensis Greville

中华盒形藻有性生殖中,雌雄性的分化分别在39—78μm宽度的细胞中产生.雌雄配子形成在早晨5-6时开始,14-20时进行减数分裂,22时形成精子,卵子同时成熟.受精多数在午夜之前进行.合子经过复大孢子萌发阶段发育成为新子代复大细胞,细胞宽度增大3.8—5.5倍.在温度20—24℃和光强度4500—5000lx条件下,雄配子发生全程一般需要15h,而合子萌发成为新子代复大细胞通常要经历12h以上时间

Seasonal Variations of Soluble Reactive Phosphorus(SRP) Uptake and Regeneration Fluxes by Microplankton in Xiamen Harbor

用32PO43-同位素稀释法测定了厦门港小型浮游生物（＜200μM）对可溶性活性磷（SrP的吸收和再生通量；并同时测定了现场的CHlA、初级生产力和各种形态磷.厦门港SrP吸收和再生通量有明显的季节变化，夏季最大、冬季最小，且SrP通量与生源要素呈现协变性；表层（0.5M）SrP通量与CHlA成较好的正相关，SrP通量的变化与浮游植物的消长密切相关.全年平均表层SrP再生能满足初级生产磷吸收的43.3%；底层（16M）再生与吸收比远大于1，SrP的再生对初级生产的调控具有重要作用.表层全年平均SrP的周转时间为88.75H，7月SrP最低，但其周转时间也最短仅为3.7H，以最大程度地利用磷维持较高的初级生产力.较低的碳、磷同化原子比及较短的SrP周转时间表明厦门港磷同化速率较高.Uptake and regeneration Fluxes of SRP were measured by using carrier Free 32 PO 4 3- in Xiamen Harbor, Fujian, China.Concentrations of Chla, three Forms of phosphorus compounds and primary productivity were measured simultaneously in situ .Obvious seasonal variations of SRP Fluxes in Xiamen Harbor surFace seawater (0.5 m) were characterized by the highest uptake and regeneration Fluxes during summer and the lowest during winter. SRP Fluxes and biological elements covaried with their seasonal variations.SRP uptake and regeneration Fluxes were positively correlated with Chla and changes of SRP Fluxes were closely related to Fluctuations of phytoplankton.On an annual basis,SRP regeneration by microplankton could meet about 43.3% of the phosphorus requirments For primary production in surFace seawater.SRP regeneration/uptake ratios were much larger than 1 in deeper layer(16 m).Recycling SRP by microplankton was very important For regulating primary production.The annual average turnover time of SRP was 88.75 h in the surFace.SRP was the lowest but turnover time was the shortest, only 3.7 h in July, and the utilization of SRP were maximized to maintain high primary production.Lower carbon/phosphorus assimilation atomic ratios and shorter turnover time of SRP indicated that there were higher phosphorus assimilation rates in Xiamen Harbor

Comparative Studies on Soluble Reactive Phosporus (SRP)uptake and regeneration fluxes by microplankton in Xiamen Harbour

应用32PO43-同位素稀释技术比较研究了厦门港3个站位4个季节表层（0.5M）浮游生物（＜200μM）对可溶性活性磷（SrP）吸收和再生通量。结果表明，SrP吸收和再生通量夏季（7月）最大，冬季（1月）最小；近河口的Ⅱ站SrP通量最大且季节波动性大，位于港口外缘的Ⅰ站SrP通量最小且季节波动小；各站SrP通量变化的差异与其不同的理化环境有关。文中还研究探讨了浮游生物在不同季节、不同站位对SrP吸收和再生通量的变化特性及生态适应机制。Uptake and regeneration fluxes of SRP by microplankton (<200μm) were studied com-paratively by using32PO3-4 isotope dilution techniques in Xiamen Harbour surface seawater (0.5m)of the three sampling stations from Oct.1989 to July 1990.Obvlous seasonal varia-tions of SRP fluxes were characterised by the highest uptake and regeraration rates during summer(July) and the lowest during winter (January).SeasonaI fluctuations of SRP fluxes were higher and much more violent at Station located nearby the estuary than those of Station near the outside edge of the Harbor.Differences of SRP fluxes variations at each station were correlated with each physical and chemical enviroments- Relatlons among SRP fluxes, microplankton and seawater environments were inquired into in the paper.Charac-ters of SRP fluxes and ecological adaptive mechanism of natural microplankton assembages in different seasons, differernt stations were interpreted preliminarily

A study on dissolved oxygen and its supplement and consumption in shrimp ponds

对对虾养殖池塘溶解氧含量及其收支情况的研究结果表明，虾池dO值具有明显的季节和昼夜变化。浮游植物光合作用是虾池溶解氧补充的主要来源，夏季最高可超过5Mg/（dM3·d）。养殖后期对虾的耗氧量占总耗氧量的34%，底质和池水耗氧量分别占30%和35%。根据虾池氧的收支情况讨论了池养对虾的生产容量。The dissoived oxygen content and budget in shirmp ponds were surveycd.The resultsshowed that signiFicant seasonal and diel variation of DO values were Found and most DOvalues were higher than 4.0mg/dm3.The main source of DO was the photosyntheticproduction.The DO supplement may be more than 5.0mg/(dm3·d) in summer.The oxygenuptaken by shrimps al the Final perlod of culture was only 34% of total oxygen depletion ofpond, while pond waler and sedlment occupied 35 % and 30% respectively.The reasonableproduction capacity of shrimp pond is discussed according to the DO budgel of shrimp pondsunder current conditions.国家自然科学基

A Survey on a Gymnodinium simplex Lohmann red tide in a shrimp pond in Pantu, Xiamen

对厦门市潘涂虾场一口虾池 1995年 4月发生的一起简单裸甲藻赤潮进行跟踪调查 ,报道赤潮期间虾池水环境因子、浮游植物生物量的变化特征以及浮游植物的种类更替 .调查显示 ,赤潮发生前浮游动物大量繁殖 ,快速消耗浮游植物进而引起池内无机营养盐 (特别是活性磷酸盐 )的积累是本次赤潮发生的重要原因 .赤潮发生期间 ,赤潮生物的暴发性增殖在抑制多数藻类 (主要是硅藻 )的同时 ,也促进了一些裸藻类、甲藻类和蓝藻类的生长繁殖 .A red tide of Gymnodinium simplex Lohmann in a shrimp pond in Pantu shrimp cultivation area, Xiamen, was investigated. The variation of the ecological factors of water and the biomass of phytoplankton in shrimp pond, and the species succession of phytoplankton during the red tide were reported. The results suggested that the accumulation of nutrients, especially reactive phosphate, caused by the mass growth of zooplankton consuming phytoplankton rapidly, was the important reason for this red tide. The rapid multiplication of red tide species during red tide restrained the majority of alga, mostly the diatom, while promoting the growth of some species belonging to Euglenophyta, Pyrrophyta and Cyanophyta.厦门市重大科技项目!招标课题《养殖对虾病毒病及其防治的研究》资助项目 ( 1993- 2 1- 1- 1