Establishment of SCAR Markers and Its Application in Identification and Au- thenticity of Parent Purity of Five Hybrid japonica Rice Lines

选用36个随机引物对“寒丰A”、“寒丰B”、“8204A”、“8204B”、“R161”等5份杂交粳稻亲本材料进行RAPD扩增,对其中特异RAPD标记片段进行克隆和测序。根据获得的特异DNA序列设计序列特征扩增区(SCAR)特异的引物,将18个RAPD标记转化成6个稳定的SCAR标记。用这些SCAR标记对亲本和杂种F1代单株进行检测,实验室检测种子纯度的结果与海南田间种植的结果基本一致。此外,应用水稻细胞质雄性不育特异的1对PCR引物,分辨出2对不育系/保持系亲本:“寒丰A”与“寒丰B”、“8204A”与“8204B”。上海市科技兴农重点攻关项目[农科攻字(2000)号第1-3号];; 上海市农业科学院青年科技基金(2000-09-02-1

Isolation and Genetic Analysis for Rice Mutants Treated with ~(60)Co γ-Ray

为了研究水稻发育的分子机制,我们利用γ射线对粳稻品种9522进行诱变,共诱变了3 000 g种子,M1代单株收种,M2代移栽5 963个株系于上海农科院.在M2代中筛选突变体,并在M3代中复选,最终得到了叶、株型育性等各类形态突变体666份.对其中162株突变体进行了遗传分析,有53.61%的突变体出现3∶1的分离,这些突变体是隐性的单位点突变.同时,这些突变体的分离也为水稻功能基因分离和功能鉴定等研究打下了良好的基础,并为水稻发育的研究提供了宝贵的材料.3,000 gram seeds of japonica cultivar 9522 background were treated with 60Co γ-Ray for the study on the molecular mechanism of rice development.Then 5 963 lines of M2 progeny were transplanted in the paddy field of Shanghai Agriculture Academy.666 mutants of the M3 progeny showed abnormal development in several organs such as flower leaf and seed,among which,162 mutants were selected randomly for further genetic analysis,53.61 percent of which showed a good fit to the expected ration of 3∶1.The result indicated that a single recessive nuclear gene might control these mutant phenotype.Isolation and genetic analysis for these mutants would pave the way for further cloning of important gene in rice and provide valuable materials for the study of rice development.国家科技部国家重点基础研究发展项目(2005CB120802)资