從價值鍊角度看日本動畫產業

從本質上來看，動畫產業屬於創意內容產業，具有巨大的經濟潛力。動畫影片除了播映產生的直接收益外，還蘊含了龐大的週邊產品授權市場。往昔研究日本動畫產業的文獻，內容多著重描述產業的發展歷史，而在產業運作模式的整體分析部分，往往著墨較少。基於個人對日本動畫產業的興趣，以及創意內容產業與日遽增的重要性，希望能透過一個整體性的架構，剖析日本動畫產業的運作方式，找出產業價值鏈發展的關鍵因素，供產界、學界往後研究日本動畫產業參考。 本研究依照產業價值鏈的研究架構，從次級資料中整理、分析日本動畫產業的發展歷程，希望能夠回答以下的研究問題： 1. 從價值鍊理論的觀點，探討日本動畫產業從1950年代到2000年代的發展歷程以及歷史背景因素的影響。希望能建構出一整體性的日本動畫產業價值鏈。 2. 藉由產業價值鏈的建構過程，釐清日本動畫產業內的重要價值活動，以及彼此之間相互影響的關係。 3. 綜合以上研究，希望能找出日本動畫產業發展的關鍵因素。 本研究之結論可摘要如下： 一、 日本動畫產業的價值鏈的內涵，可分為五個環節來描述 1. 原始創意：「原始創意」乃是動畫作品製作流程中，最早期投入的「原料」。包含動畫作品故事的題材選用、各項設定如人物、場景、美術設定、以及說故事的表現手法。 2. 資源募集：原料備妥後，為了要進入製作動畫的階段，還必須要有資金、人才、以及技術的到位，稱之為「資源募集」。資源募集包含了資金的籌措方式、人才培育的制度機構、以及動畫技術的創新突破。 3. 動畫製作：動畫製作的階段可再細分為許多的細項，但是對產業發展的過程來說，關鍵點不在於製作流程中某一項活動的創新，而是整體製作流程的演進，包含：垂直整合、專業分工、以及動畫製程的改變。 4. 內容流通：動畫作品製作完成後，需要經由種種的通路，將作品的影響力傳播出去。傳播的管道不限於一般的播送通路如電視、電影，也有可能是通過參加影展、參加專為動畫作品設置的競賽、接受某些動畫獎項的評審。此外，在內容流通的過程中，政府法規所扮演的角色，可能是助力，亦有可能是阻力。 5. 價值回收：經過內容流通的階段後，動畫作品的影響力傳播出去，這時候廠商開始透過許多不同的管道，進行價值回收的活動。除了直接針對傳播媒體收取播送的權利金、透過電影院的票房分紅外，透過肖像授權的方式所衍生出來的周邊事業，更是龐大的商機所在。 二、 價值鏈上的各環節存在著互相鏈結的現像 6. 「原始創意」環節的改變，會影響「內容流通」、「價值回收」等環節。 7. 「資源募集」環節的改變，會影響「原始創意」、「動畫製作」、「內容流通」、「價值回收」等全部環節。 8. 「動畫製作」環節的改變，會影響「原始創意」、「內容流通」等環節。 9. 「內容流通」環節的改變，會影響「原始創意」、「價值回收」。 10. 「價值回收」環節的改變，會影響「內容流通」環節。 三、 日本動畫產業發展的關鍵因素，在於完整價值鏈的建立，以及價值鏈內不同環節之間鏈結的強固 11. 在「原始創意」部分，日本動畫題材與表現手法的豐富，是發展歷程每個階段不斷累積的成果。 12. 「資源募集」部分，資金的來源多元、人才的培育制度化、技術不斷的創新，使得日本動畫產業在這部份相當具有競爭力。 13. 「動畫製作」部分，隨著流動通路的改變能夠採用不同的模式；並且發展出兩大軸線：虫製作的「有限動畫」和吉卜力工作室的「精緻製作」。 14. 「內容流通」和「價值回收」部分，新的動畫放映通路不斷的出現，伴隨著各種價值回收的手法開發出來，使得日本動畫產業在周邊商品的行銷上具有相當的能耐。 四、 日本動畫產業價值鏈的分析方式，不僅可應用於動畫產業，更可應用於其他相關之文化創意產業範疇 15. 本研究乃是依據Porter所提出之價值鏈理論作為建構產業價值鏈之工具，並輔以文獻探討中關於文化創意產業經濟特性以及電影產業價值鏈的回顧，將原先Porter之「製造業」思維之產業價值鏈，轉化為「軟體產業」思維之產業價值鏈。 16. 凡是文化創意產業中具有與動畫產業類似之創意投入、內容產製、流通、價值回收之產業，諸如電影產業、遊戲產業、音樂產業……等，都可以本研究所提出之產業價值鏈之分析模式，作為產業發展分析之工具。Essentially, animation industry, as a category of creative industries, has great economic potential. They include direct revenue from exhibitions and and there is a big market of related products. Most documents about Japanese animation industry focus on the history of industry development; total analysis of industrial operation is relatively less emphasized. Based on my personal interests in Japanese animation industry and the increasing importance of creative industries, this research will analyze the operation of Japanese animation industry and figure out the key factors in its value chain development. The objective of this research is to answer the following questions: 1. From the viewpoint of value chain theory, to study the history of Japanese animation industry between 1950 to 2000 in order to build a complete industrial value chain. 2. Through the building process of industrial value chain, identify the key value activities and the mutual effects among them. 3. Upon analysis, identify the key factors of Japanese animation industry development. The conclusions of this research can be summarized as below: A. The contents of the value chain of the Japanese animation industry can be described as five core elements. 1. Original creativity: Original creativity is the raw materials of the animation film production which are injected at the early stage, including the story, characters, scene, and art. 2. Resources collection: Before entering the stage of production, some resources are essential, such as capital, human resource, and technology. 3. Production: From the industrial viewpoint, we focus on the change of production process, including vertical integration, specialization and division of labor. 4. Contents distribution: Through various kinds of channels, the influences of animation films are spread out. The channels include television, theaters, exhibitions, contests, and awards. Also, the government and laws play the roles as help or blockage. 5. Value recycle: After the influence of animation films are spread out, enterprises begin to gain value recycle through several ways, such as premium, distribute profits, and especially the sales of related products. B. The five elements of the value chain are connected with different chains. 6. Changes of “Original creativity” have effects on “Contents distribution” and “Value recycle”. 7. Changes of “Resources collection” have effects on “Original creativity”, “Production”, “Contents distribution“ and “Value recycle”. 8. Changes of “Production” have effects on “Original creativity” and “Contents distribution”. 9. Changes of “Contents distribution” have effects on “Original creativity” and “Value recycle”. 10. Changes of “Value recycle” have effects on “Contents distribution”. C. The key factors of Japanese animation industry development are the construction of complete industrial value chain and the strong connections among the chains of different elements. 11. In the part of “Original creativity”, the rich contents about stories and performances of Japanese animations are the results of long-term accumulation. 12. In the part of “Resources collection”, multiple sources of capital, systematic education of human resource and continuous innovations of technology make Japanese animation industry very competitive. 13. In the part of “Production”, variable production processes are adopted according to different exhibition channels. There goes two major production processes: “Limited animation” and “Refined animation”. 14. In the part of “Contents distribution” and “Value recycle”, continuous invention of new exhibition channels and methods of value recycle make the Japanese animation industry very proficient in marketing the related products. D. The analysis methods of this research can apply to not only the animation industry, but also to the other fields within the creative industries. 15. This research transforms the “manufacturing” industrial value chain model mentioned by Michael E. Porter into the “software” one, based on the reviews of documents. 16. The analysis methods of this research work on those industries with similar creativity injection, production, distribution, and value recycle as the animation industry, such as movie, music, video game industries

[[alternative]]Development of renal function evaluation by HPLC with copper based ECD

碩士[[abstract]]　　　近幾年因食安的問題、工業重金屬的汙染及藥物的濫用，全球腎臟病人口不斷攀升，如何能準確測量尿酸及肌酸酐含量並計算腎臟廓清率，成為臨床上一重要的課題；本研究以銅電極為基礎之薄層電化學偵測器做為高效液相層析之偵測器，開發能同時偵測人體血液及尿液中尿酸及肌酸酐含量之偵測方法，並可計算腎臟廓清率，完成臨床之腎功能評估偵測方法；此研究藉由施加氧化工作電位，使電極表面形成難溶性氧化銅層，當尿酸或肌酸酐隨流動相經過電極表面時，與電極表面的難溶性氧化銅配位形成游離性錯合物，而電極表面在-0.1V的電壓下會再生成新的氧化銅層，藉由偵測所量測的氧化電流的改變，間接偵測人體血液及尿液中的尿酸及肌酸酐含量；本法其他最佳偵測條件，緩衝溶液流動相為50mM磷酸-75mM醋酸緩衝溶液，酸鹼值為pH7.4，流動相流速為1.0ml/min，樣品注射體積為20μl，利用最佳化條件，達到最佳偵測靈敏度。在最佳化的偵測條件下進行尿酸及肌酸酐的偵測，所得到的偵測分析特性之尿酸偵測極限為0.11μM，肌酸酐為0.50μM，尿酸及肌酸酐線性範圍皆為2.5-500μM，偵測靈敏度為尿酸168.06(nA/mM)，而肌酸酐為11.74(nA/mM) (S/N=3)；此偵測方法之線性範圍足以涵蓋人體血液中尿酸及肌酸酐含量之正常範圍，且實驗最佳化條件在真實樣品測試中，也足以使尿酸及肌酸酐與人體血液及尿液中其他雜質完全分離，證明此偵測方法能被作為臨床檢測的工具。[[abstract]]　　　Both of uric acid(UA) and creatinine(Cr) are important indicator of renal function. For the past years, food additives, industry heavy metal pollution and drug abuse make a severe affect for human health and the population of renal failure is getting higher. For these reason, how to calculate the estimated glomerular filtration rate(eGFR) is always on critical. In this scheme, it developes a determination of HPLC-Cu-ECD to detect UA and Cr simultaneous in human serum and urine and estimate eGFR further. Some reports presented UA and Cr can be chelated with Cu(I) and Cu(II). In this determination, it supplied an oxdation voltage -0.1V(vs. Ag/AgCl) to create the rigid Cu(II) layer on electrode surface. A 50mM phosphate - 75mM acetate buffer solution was used to be a mobile phase at pH7.4 for flow rate 1.0ml/min. UA or Cr will chelate with Cu(II) of the electrode surface then desorption. A oxidative current will be generated when the copper electrode surface formation rearrangement. The detection limit of the determination are presented 0.11 and 0.50μM for UA and Cr which was calculated from 12 times blank injections. The linear range of UA is form 2.5-500μM and Cr is from 10-500μM. The linear range of UA and Cr are over the human serum normal range of UA and Cr. The analytical sensitivity of UA and Cr are 168.06nA/mM and 11.74nA/mM respectively. Both of UA and Cr can be separated other impurities in this scheme that proved it is useful on clinical determination of renal function.[[tableofcontents]]目 錄 第一章 緒論........................................................................................................ 1 1-1 腎功能........................................................................................................... 2 1-2 常規腎功能檢測........................................................................................... 5 1-3 尿酸............................................................................................................. 10 1-4 肌酸酐......................................................................................................... 12 1-5 尿酸及肌酸酐檢測方法............................................................................. 14 1-5-1 尿酸檢測方法.................................................................................. 15 1-5-2 肌酸酐檢測方法.............................................................................. 21 1-5-3 同時偵測尿酸及肌酸酐偵測方法.................................................. 25 1-6 層析法......................................................................................................... 28 1-6-1 薄層層析法...................................................................................... 29 1-6-2 氣相層析法...................................................................................... 30 1-6-3 液相層析法...................................................................................... 30 1-6-3-1 分配層析法......................................................................... 31 1-6-3-2 吸附型層析法..................................................................... 33 1-6-3-3 離子層析法......................................................................... 34 1-6-3-4 分子排除式層析法............................................................. 34 1-6-3-5 親和層析法......................................................................... 35 1-6-4 超臨界流體層析法.......................................................................... 36 1-7 研究目的..................................................................................................... 37 第二章 實驗...................................................................................................... 38 2-1 儀器............................................................................................................. 38 2-1-1 電化學實驗...................................................................................... 38 2-1-2 高效液相層析法.............................................................................. 38 2-1-3 其他.................................................................................................. 39 2-2 藥品............................................................................................................. 40 2-3 實驗前處理................................................................................................. 41 2-3-1 電極製備.......................................................................................... 41 2-3-2 管柱平衡.......................................................................................... 41 2-3-3 標準品製備...................................................................................... 41 2-3-4 尿液及血液真實樣品前處理.......................................................... 42 2-4 實驗流程設計............................................................................................. 43 2-4-1銅電極偵測機制............................................................................... 43 2-4-2銅電極工作電位探討....................................................................... 43 2-4-3流動相酸鹼值探討........................................................................... 44 2-4-4緩衝溶液種類的探討....................................................................... 45 2-4-5緩衝溶液濃度探討........................................................................... 45 2-4-6緩衝溶液修飾劑探討....................................................................... 46 2-4-7磷酸-醋酸鈉緩衝溶液探討.............................................................. 47 2-4-8實驗流速探討................................................................................... 48 2-4-9樣品迴路體積探討........................................................................... 48 2-4-10分析特性......................................................................................... 49 第三章 結果與討論.......................................................................................... 50 3-1銅電極偵測機制................................................................................... 51 3-2銅電極工作電位探討........................................................................... 56 3-3流動相酸鹼值探討............................................................................... 59 3-4緩衝溶液種類探討............................................................................... 64 3-5緩衝溶液濃度探討............................................................................... 67 3-6緩衝溶液修試劑探討........................................................................... 69 3-7磷酸-醋酸鈉緩衝溶液探討.................................................................. 75 3-8實驗流速探討....................................................................................... 77 3-9樣品迴路體積探討............................................................................... 78 3-10分析特性............................................................................................. 80 資料來源............................................................................................................ 86 附錄.A................................................................................................................. 92 圖 表 圖1-1 尿酸於人體中的代謝機制..................................................................... 12 圖1-2 肌酸及肌酸酐在人體中的代謝機制..................................................... 14 圖3-1銅電極偵測機制探討.............................................................................. 54 圖3-2銅電極於流動注射系統中的偵測........................................................... 55 圖3-3銅電極工作電位探討.............................................................................. 58 圖3-4酸鹼值對偵測背景電流值的影響.......................................................... 61 圖3-5偵測酸鹼值探討...................................................................................... 62 圖3-6尿酸與肌酸酐滯留時間.......................................................................... 63 圖3-7緩衝溶液種類探討.................................................................................. 66 圖3-8緩衝溶液濃度探討.................................................................................. 68 圖3-9醋酸根的影響.......................................................................................... 72 圖3-10緩衝溶液修試劑對訊號強度的探討.................................................... 73 圖3-11修試劑對尿酸及肌酸酐的影響............................................................ 74 圖3-12磷酸-醋酸鈉溶液探討........................................................................... 76 圖3-13樣品迴路體積探討................................................................................ 79 圖3-14尿酸檢量線與肌酸酐檢量線................................................................ 83 圖3-15真實樣品................................................................................................ 84 圖3-16相關係數................................................................................................. 85 表.1腎絲球廓清率與腎病程度分析表............................................................... 4 表.2腎絲球疾病的分類....................................................................................... 5 表.3尿液尿蛋白檢測對照表............................................................................... 7 表.4尿酸與肌酸酐於不同緩衝溶液中之訊號半峰寬..................................... 65 表.5最佳化分析條件......................................................................................... 81 表.6分析特性..................................................................................................... 82[[note]]學號: 603160184, 學年度: 10

網路互動式3D應用-以室內傢俱擺設為例

[[abstract]]因現今電腦科技資訊的發達，使得3D虛擬實境日益普遍，突破以往大多只能使用2D的網際網路。3D虛擬實境「室內設計互動式平臺」突破傳統，不用請設計師設計，還可在家不用出門，依個人需求、喜好、構想來設計屬於自已的格局。以往要請設計師幫忙設計屬於自已的空間時，常常要與設計師一而再，再而三的討論，才可以漸漸形成自已夢想中的家。我們運用了3DS MAX建構了許多傢俱模型和Virtools Dev的3D整合、動作指令、控制語法、攝影機等方法建立了「室內設計互動式平臺」提供了坪數大小、空間、格局、傢俱種類，讓使用者在家就可以自行更換成自已想要的傢俱種類與擺設