Calculation of Young's Modulus and Coefficient of Linear Expansion on Several Solid Solution with Cubic Structure

杨氏模量和线膨胀系数是表征金属材料重要的物理量。但是，目前已有的二元及多元合金固溶体的杨氏模量和线膨胀系数的实验数据十分有限。因此，利用合理的理论模型来实现对杨氏模量和线膨胀系数的计算并建立性能数据库十分重要，能够为材料设计提供必要的基础信息。本课题组在应用相图计算技术建立热力学数据库和指导合金的成分设计方面取得了不错的成效。本研究基于相图计算的研究方法，考虑温度和成分两个变量的影响，开展合金溶体相的杨氏模量和线膨胀系数的计算。其具体研究成果如下： (1)借鉴计算相图(CALPHAD)方法的研究思路，构建了二元及三元合金溶体相的杨氏模量和线膨胀系数的计算模型。 (2)运用杨氏模量的半经验模...Young's modulus and coefficient of linear expansion are the important physical properties of metal materials. But experimental data of the binary and multicomponent alloy solid solution is limited. Therefore, establishing reasonable theoretical model to calculate young's modulus and linear expansion coefficient is very important for establishing the property database. In our group, we have achieve...学位：工学硕士院系专业：材料学院_材料学学号：2072012115004

The effect of low glucose and high lactic acid on the survival of Hela

目的:探讨低糖高乳酸环境对宫颈癌细胞生存的影响,以及对EGFR-mTOR通路的调节作用。方法:将Hela细胞培养于常糖(葡萄糖10mmol/L); 、低糖(葡萄糖3mmol/L)、高乳酸(葡萄糖10mmol/L,乳酸2.5mmol/L)、低糖高乳酸(葡萄糖3mmol/L,乳酸2.5mmol/; L)4种环境下,CCK-8法测定Hela细胞的生长抑制率,流式细胞术测定细胞周期。荧光实时定量PCR法检测EGFR和mTOR; mRNA水平表达。结果:与常糖组相比,高乳酸组的细胞抑制率显著升高(P<0.01),48h细胞G_1/G_0期比例显著升高(P<0.01),细胞; 凋亡率、EGFR和mTOR; mRNA表达水平均无变化。与常糖组相比,低糖组的细胞抑制率显著升高(P<0.01),48h细胞凋亡率显著降低(P<0.01),细胞各周期比例变化; 与常糖组无差异,EGFR表达水平降低(P<0.05)。与常糖组相比,低糖高乳酸组的细胞抑制率显著升高(P<0.01),但低于低糖组(P<0.01; )和高乳酸组(P<0.05),48h G_1/G_0期比例显著升高(P<0.01),细胞诱导凋亡率显著升高(P<0.01),EGFR和mTOR; mRNA表达水平均显著升高(P<0.01)。结论:Hela细胞在低糖高乳酸环境中的存活状况好于单纯低糖和单纯高乳酸环境,且伴随着EGFR和mTO; R基因表达水平上升。Objective:To explore the effect of the low glucose and high lactic acid; environment of survival of cervical carcinoma cells,and the regulation; of EGFR-mTOR signal way.Methods:Hela cells were cultured in four; conditions:normal glucose (glucose 10mmol/L),low glucose (glucose; 3mmol/L),high lactic acid (lactic acid 2.5mmol/L) and low glucose add; high lactic acid (glucose 3mmol/L,lactic acid 2.5mmol/L).Growth; inhibition rate of Hela cell was determined by CCK-8.Flow cytometry; (FCM)were performed to evaluate the cell cycle.The expression levels of; EGFR and mTOR mRNA were detected using real-time quantitative polymerase; chain reaction (real-time PCR).Results:Compared with those in regular; sugar environment,the cell growth inhibition rates were significantly; increased in high lactic acid environment(P<0.01).At 48h,the rate of; G_1/G_0 was significantly increased (P<0.01),while the apoptosis rate of; the cells and the expressions of EGFR and mTOR mRNA had no; change.Compared with those in regular sugar environment,the cell growth; inhibition rates were significantly increased in high lactic acid; environment,(P<0.01),while lower than those in low glucose; environment(P<0.01) and in high acid environment(P<0.05).The rates of; G_1,G_2 and S phase had no change.The expressions of EGFR mRNA was; reduced (P<0.05).The cell growth inhibition rates were significantly; increased in high lactic acid add low glucose environment (P<0.01).At; 48h,the apoptosis rate and the rate of G_1/G_0 were significantly; increased (P<0.01),while the EGFR and mTOR expression levels were also; increased (P<0.05).Conclusion:Hela in the low glucose add high lactic; acid survives better than those in low glucose and in high lactic acid; environment,while the expression of EGFR and mTOR is increased.泉州市科技计划立项项目; 国家面上项

Monthly changes in the caloric values of the leaves of four shrubby and four tree-dwelling palmae species

对 4种灌木状棕榈植物 (香棕、袖珍椰子、棕竹、江边刺葵 )与 4种乔木状棕榈植物 (假槟榔、董棕、国王椰子、大王椰子 )叶的热值和灰分含量的月变化进行了研究。结果表明 :( 1 ) 8种植物叶的灰分含量存在差异且具有不同的月变化 ;从灰分含量的比较看 ,乔木类的 4种植物叶平均灰分含量分别为 :假槟榔 1 3.64%± 2 .94%、董棕 9.74%± 1 .90 %、国王椰子 9.1 2 %± 1 .1 8%、大王椰子 8.69%± 3.5 5 % ;灌木类的 4种植物叶平均灰分含量分别为 :棕竹 8.73%± 2 .5 2 %、袖珍椰子 8.67%± 1 .1 9%、香棕 8.63%± 1 .2 0 %、江边刺葵 7.60 %± 0 .98% ,乔木类植物 (除大王椰子外 )叶平均灰分含量高于灌木类的植物 ;( 2 )从干重热值的月变化来看 ,分 3种类型 :江边刺葵与棕竹有相反的变化趋势 ;假槟榔、董棕、袖珍椰子的月变化趋势相同 ;国王椰子、香棕、大王椰子具有各自的变化趋势 ;在 8种棕榈植物中 ,除香棕外 ,灌木类的植物平均干重热值大于乔木类 ;灌木类中 ,茎单生的江边刺葵干重热值高于茎丛生的棕竹、香棕和袖珍椰子 ;( 3)假槟榔、袖珍椰子、大王椰子的干重热值与灰分含量具有极显著的线性相关 ( P<0 .0 1 ) ,棕竹的干重热值与灰分含量有显著的线性相关 ( P<0 .0 5 ) ;而香棕、董棕、江边刺葵、国王椰Monthly changes in the ash contents and caloric values of the leaves of 8 Palmae species(four shrubby species: Arenga engleri, Chamaedorea elegans, Rhapis excelsa and Phoenix roebelenii; four tree dwelling species: Archontophoenix alexandrae, Caryota urens, Ravenea rivularis and Roystonea regia) were discussed in this paper, and the results showed as follows: (1) The ash contents of the 8 Palmae species were different and varied with season; the annual average ash contents of the four tree dwelling species were 13 64%±2 94% for Archontophoenix alexandrae, 9 74%±1 90% for Caryota urens, 9 12%±1 18% for Ravenea rivularis, and 8 69%±3 55% for Roystonea regia; those of the four shrubby species were 8 73%±2 52% for Rhapis excelsa, 8 67%±1 19% for Chamaedorea elegans, 8 63%±1 20% for Arenga engleri, and 7 60%±0 98% for Phoenix roebelenii, respectively; the average ash contents of the four tree dwelling species (except for Roystonea regia ) were higher than those of the four shrubby species; (2) The 8 species had three types of monthly gross caloric value change trends: Archontophoenix alexandrae, Caryota urens and Chamaedorea elegans had similar monthly change trend, while Phoenix roebelenii and Rhapis excelsa had the opposite trends of monthly changes; the other three species ( Ravenea rivularis, Arenga engleri and Roystonea regia ) each had its own changing trend. The shrubby species had higher gross caloric value than the tree dwelling species except for Arenga engleri, of the shrubby species, Phoenix roebelenii (monaxial stem) had the highest gross caloric value (than Arenga engleri, Chamaedorea elegans and Rhapis excelsa (clumpy stem)); Seasonal changes in gross caloric values varied with various species; (3) Gross caloric values were correlated remarkably with ash contents for Archontophoenix alexandrae, Chamaedorea elegans and Roystonea regia (P <0 01), they were correlated for Rhapis excelsa (P <0 05), too, whereas there was no significant correlation between gross caloric values and ash contents for other species; (4) the average ash free caloric values of the shrubby species were 22 65±0 59 kJ/g for Phoenix roebelenii, 22 19±0 48 kJ/g for Chamaedorea elegans, 21 94±0 76 kJ/g for Rhapis excelsa and 21 35±0 75kJ/g for Arenga engleri, respectively; those of the tree dwelling species were 21 76±0 55kJ/g for Ravenea rivularis, 21 20±0 72kJ/g for Caryota urens, 21 12±0 52kJ/g for Archontophoenix alexandrae and 20 98±0 55 kJ/g for Roystonea regia respectively; the shrubby species had higher ash free caloric value than the tree dwelling species except for Arenga engleri. The seasonal changes in ash free caloric values were different from those in gross caloric values due to various ash contents in the leaves of the 8 Palmae species.国家建设部资助项目;; 厦门市园林局资助项目~

低糖高乳酸环境下吉非替尼诱导HeLa细胞EGFR-TKI耐药的研究

目的探讨低糖高乳酸微环境对表皮生长因子酪氨酸激酶抑制剂(EGFR-TKI)抑制HeLa细胞的影响和可能机制。方法HeLa细胞在常糖(葡萄糖10; mmol/L)和低糖高乳酸(葡萄糖3 mmol/L +乳酸2.5 mmol/L)环境下培养,并分别给予2.67; mumol/L吉非替尼干预。采用CCK-8法测定HeLa细胞生长抑制率,流式细胞术检测细胞周期,荧光定量RT-PCR检测EGFR和mTOR; mRNA水平的表达。结果与常糖组比较,低糖乳酸组24 h和72 h细胞抑制率均显著升高(P < 0.01),48; h细胞抑制率略高于常糖组。与吉非替尼阴性组比较,常糖+吉非替尼组和低糖乳酸+吉非替尼组在24、48、72 h三个时点细胞抑制率均显著上升(P <; 0.01)。与常糖组相比,低糖乳酸组48 h细胞诱导凋亡率显著上升(P <; 0.01),低糖乳酸+吉非替尼组细胞诱导凋亡率较低糖乳酸组显著降低(P < 0.01)。与常糖组比较,低糖乳酸组存活细胞的EGFR和mTOR; mRNA表达水平上调(P < 0.05)。常糖+吉非替尼组的EGFR和mTOR mRNA水平均上调(P <; 0.05)。与低糖乳酸组比较,低糖乳酸+吉非替尼组的EGFR和mTOR mRNA上调水平有显著差异(P <; 0.01)。结论高乳酸低糖环境下吉非替尼可大幅度上调存活HeLa细胞EGFR和mTOR表达水平,可能是诱导HeLa细胞抵抗EGFR-TKI的机制; 。泉州市第一医院青年科研; 泉州市科技计划项目; 国家面上项

间充质细胞外泌体促进小鼠胰岛内皮细胞血管生成的研究

目的探讨间充质细胞（MSC）外泌体对低氧条件下胰岛内皮细胞（MS-1）血管生成的影响。方法 MSC无血清低氧条件培养48 h,超滤离心法富集条件培养基中的外泌体,采用电镜和Western Blot的方法进行鉴定;通过血管形成试验比较分析不同条件下:常氧培养组（NOR组,21%O2、5%CO2）、低浓度氧培养组（HYP组,2%O2、5%CO2）、外泌体+低浓度氧共培养组（HYP+EXO组,2%O2、5%CO2）,MS-1细胞的血管形成能力;image J软件分析血管形成长度;PCR、Q-PCR检测血管内皮生长因子（VEGF） RNA水平的表达,Western Blot检测VEGF、HIF1α蛋白水平表达以及mTOR信号通路激活情况。采用单因素方差分析和SNK-q检验统计学分析。结果超滤离心法富集的MSC条件培养基中的外泌体,大小为30～100 nm,表达CD9,CD63,CD81等外泌体表面标志物;血管形成试验结果显示,低氧促进MS-1血管生成,HYP+EXO组形成明显的血管网状结构;HYP+EXO组血管形成相对长度（2386.0±137.7）像素与NOR组（393.3±174.2）像素和HYP组（1467.0±230.0）像素相比增强,差异有统计学意义（t=12.30,P=0.0065;t=15.74,P=0.0040）; PCR结果显示,HYP+EXO组VEGF相对表达量（20.26±9.972）较常氧对照组（1.000）和低氧组（6.521±3.501）均增强,差异有统计学意义（t=5.462,P=0.0009;t=4.238,P=0.0038）;同时,Western Blot结果显示VEGF蛋白水平表达升高,HIF1-α表达上调,mTOR发生磷酸化。结论 MSC外泌体可促进低氧条件下的小鼠胰岛内皮细胞血管生成。MSC外泌体可能通过上调HIF1-α,调节VEGF表达,激活mTOR信号通路,促进胰岛内皮细胞血管生成。国家自然科学基金青年项目(81601618);;福建省自然科学基金面上项目(2016J01582、2016J01580、2018J01349);;福建省科技创新联合资金重大项目(2017Y9127

骨髓间充质细胞联合PDMS支架构建移植胰岛微环境的实验研究

目的为了提高移植胰岛的活性和功能,构建适合移植胰岛生存的微环境。方法采用聚二甲基硅氧烷(PDMS)和氯化钠晶体构建三维支架,联合骨髓间充质细胞(MSCs)、纤维蛋白和胰岛共同构建迷你\"人工胰腺\"。采用链脲佐菌素(STZ)诱导的糖尿病大鼠移植模型评价效果,将\"人工胰腺\"移植到糖尿病大鼠大网膜内,对照组行假手术,术后隔天监测移植大鼠血糖水平;数据采用t检验和曼-惠特尼U检验。结果用PDMS构建的三维巨孔支架,支架内可见大量不规则孔洞空间。胰岛和MSCs可成功装载入支架内,HE染色结果显示,支架孔内存在胰岛,胰岛周围包绕有MSCs。糖尿病大鼠大网膜内移植结果显示,移植后各时间点(1,3,5,7 d),\"人工胰腺\"移植组糖尿病大鼠血糖水平分别为(278.70±86.06) mg/dl、(323.50±44.29) mg/dl、(283.30±74.00) mg/dl、(304.80±13.33) mg/dl,较假手术对照组(606.00±52.40) mg/dl、(589.70±55.78) mg/dl、(615.00±54.84) mg/dl、(630.30±48.17) mg/dl均降低,差异具有统计学意义(t=7.96、9.15、8.82,U=0.00,P均<0.01)。结论 MSCs联合PDMS三维支架构建的微环境,可为移植胰岛提供生存的环境,为临床开展胰岛移植提供新的策略

Seasonal changes in the caloric values of the leaves of seven mangrove species at Futian, Shenzhen

对广东深圳福田红树林自然保护区的 7种红树植物叶热值和灰分含量的季节变化进行了研究 .结果表明 :( 1 ) 7种红树植物叶的灰分含量存在差异 ,且具有一定的季节变化 ;( 2 ) 7种红树植物叶中 ,大戟科的海漆、梧桐科的银叶树这两种红树植物叶的干重热值与灰分含量具有极显著的线性相关 ,其余 5种红树植物叶的干重热值与灰分含量相关性不显著 ;( 3)从干重热值的季节变化来看 ,2 0 0 1年 1月 (冬季 )、2 0 0 1年 4月 (春季 ) 7种红树植物叶的干重热值与灰分含量有极显著的线性相关 ,2 0 0 0年 7月 (夏季 ) 7种红树植物叶的干重热值与灰分含量有显著的线性相关 ,2 0 0 0年 1 0月 (秋季 ) 7种红树植物叶的干重热值与灰分含量相关性不显著 ;( 4 ) 7种红树植物叶的去灰分热值与干重热值存在不同的季节变化趋势 ,但 7种红树植物叶的去灰分热值均是秋季最高Seasonal changes in the caloric values and ash contents of the leaves of seven mangrove species at Futian, Shenzhen are discussed, the result shows as follows: (1)there were differences in ash contents of the leaves of seven mangrove species;(2)gross caloric values( y ) correlate remarkably with ash contents( x )for Excoecaria agallocha leaves and Heritiera littoralis leaves, respectively. There is no significant relation between gross caloric values and ash contents for other mangrove species; (3)for seasonal changes in the gross caloric values, there is extremely significant relation between gross caloric values and ash contents in January and April 2001, significant relation in July 2000, no significant relation in October 2000, respectively; (4)seasonal changes in ash free caloric values are different form those in gross caloric values, but seasonal ash free caloric values in the leaves of seven mangrove species are all the highest in autumn.中港合作资助项目 (K810 71

Filtration of the Cabbage Butterfly(Pieris rapae) N-Acetyl-β-D-Glucosaminidase Inhibitor

以菜青虫n-乙酰-β-d-氨基葡萄糖苷酶(nAgASE)为研究对象,比较了不同菜青虫部位来源及不同生长期的菜青虫nAgASE活性.结果表明,5龄虫和预蛹表皮来源的nAgASE比活最高.选取肉桂酸等天然化合物及其衍生物以及一些具有抗虫或杀虫活性的植物的粗提物,以其对菜青虫nAgASE的抑制作用为指标来粗筛nAgASE抑制剂.结果表明茴香酸、异丙基苯甲酸、间-羟基苯甲酸、2,4-二甲氧基苯甲酸以及肉桂酸对该酶具有一定的抑制效果,6 MMOl/l浓度可以分别使酶活力被抑制35.3%、25.9%、26.7%、29.1%和20.0%;银杏外种皮粗提物有较强的抑制效果,0.4 Mg/Ml的银杏酸可以让酶活力几乎完全丧失.上述结果提示有望从粗筛得到的几种植物中进一步分离纯化出高效的nAgASE抑制剂,为开发新型生物农药提供理论依据.We compared the specific activity of N-Acetyl-β-D-glucosaminidase(NAGase) from the larva of cabbage butterfly(Pieris rapae),the results showed that the specific activity of NAGase from the epidermis of cabbage butterfly larva was the highest among the specific activities of the NAGase from cabbage butterfly larva different parts,and the specific activity of NAGase from the five-age larva and pre-pupae are higher than other stages of the cabbage butterfly.Through the research on inhibitor effects of natural compounds and their derivants on NAGase activity and crude extract from plants on NAGase activity,the result indicated p-anisic acid,p-Isopropyl benzoic acid,m-Hydroxy benzoic acid,2,4-Dimethoxy benzoic acid and cinnamic acid,each 6 mmol/L,can obtain inhibit rate 35.3%,25.9%,26.7%,29.1% and 20.0%,respectively.And the effects of other compounds are far from satisfactory.The research on the inhibition effect of plants crude extract showed that crude extracts from the episperm of gingko billoba with water had strong inhibition effects.This research would serve as a theoretic basis for the development of bio-pesticide which had the major components of insect chitinolytic enzyme inhibitors.福建省自然科学基金(2006J0078);厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室开放项目(2007102)资