虎伯寮南亚热带雨林自然保护区的竹类资源及开发利用

福建省南靖县虎伯寮南亚热带雨林自然保护区的竹类资源丰富 ,有 8属 2 0种 3变种 5栽培型 ,其中梅花毛竹 ( Phyllostachys heterocycla cv.obtusangula)为福建竹类新分布 ;主要的竹林类型有毛竹林、麻竹林、绿竹林、石竹林、单竹林。竹类资源的开发利用具有广阔的前

Successive modification of polydentate complexes gives access to planar carbon-and nitrogen-based ligands

以碳和氮为键合原子的多齿螯合物是配合物家族中非常重要的一类。具有更高齿数的平面构型NC螯合物实例相对较少，代表性的例子为四齿金属碳卟啉类化合物，这类螯合物以其独特的结构和丰富的物理化学性质引起广泛关注。然而平面五齿、六齿的NC螯合物由于几何构型“拥挤”导致合成难度高，该工作从含三元环内金属卡宾结构的CCCC型碳龙配合物出发，利用经典有机反应（炔烃对金属卡宾的插入反应），成功地实现了CCCCN/NCCCN型平面五齿螯合物的合成。这一研究为高配位型螯合物的合成提供了新思路并为平面五齿螯合物家族添加新成员。特别是，这些高配位型螯合物在可见光和近红外区域均有较好的吸收，表现出良好的光声成像、光热转换及声动力学性能。 该研究工作在张弘教授指导下完成，第一作者为iChEM博士后周小茜。该工作充分体现了多学科协同研究优势：相关化合物的合成、表征及理论计算工作由周小茜博士完成；声动力学性能研究由厦门大学公共卫生学院庞鑫博士及刘刚教授完成；光声成像研究由厦门大学公共卫生学院聂立铭教授完成。iChEM fellow卓庆德博士、博士生卓凯玥、陈志昕参与了部分实验工作。夏海平教授、香港科技大学林振阳教授和南京大学朱从青教授对研究工作给予了大力支持。【Abstract】Polydentate complexes containing combinations of nitrogen and carbon (N and C) ligating atoms are among the most fundamental and ubiquitous molecules in coordination chemistry, yet the formation of such complexes with planar high-coordinate N/C sites remains challenging. Herein, we demonstrate an efficient route to access related complexes with tetradentate CCCN and pentadentate CCCCN and NCCCN cores by successive modification of the coordinating atoms in complexes with a CCCC core. Combined experimental and computational studies reveal that the rich reactivity of metal-carbon bonds and the inherent aromaticity of the metallacyclic skeletons play key roles in these transformations. This strategy addresses the paucity of synthetic approaches to mixed N/C planar pentadentate chelating species and provides valuable insights into the synthesis of carbon-based high-coordinate complexes. Furthermore, the resulting complexes are the examples of organometallic species with combined photoacoustic, photothermal, and sonodynamic properties, which makes them promising for application in related areas.This research was supported by the National Natural Science Foundation of China (Nos. 21572185, 21561162001, and 81571744), the Research Grants Council of Hong Kong (N_HKUST603/15), the Excellent Youth Foundation of Fujian Scientific Committee (2018J06024), and the Fundamental Research Funds for the Central Universities (20720170065).该工作得到国家自然科学基金委、香港研究资助局、福建省自然科学基金、厦门大学校长基金的资助

Study on Protein Expression and Physiological Differences Among Four Morphologic Filaments of Spirulina platensis

无论是在生产还是在实验室培养条件下,螺旋藻多形性变异现象普遍存在.本研究从钝顶螺旋藻(SPIrulInA PlATEnSIS)中分离纯化获得了4种不同形态的藻丝体,分别是直线形藻丝体(SPl)、低度螺旋形藻丝体(SPS-l)、中度螺旋形藻丝体(SPS-M)和高度螺旋形藻丝体(SPS-H).对这4种不同形态螺旋藻的生长速率、光合作用、叶绿素A(CHl A)含量分别进行研究,发现这4种不同形态藻丝体在生长和光合生理上存在明显的差异.而总蛋白的SdS-PAgE分析也表明,不同形态藻丝体在蛋白的表达上也有差异,其中34 ku的蛋白只在直线形藻丝体SPl中大量表达.Polymorphic variation of Spirulina has frequently been observed under laboratory and industrial condition.In our laboratory conditions,four morphological filaments of Spirulina platensis,which were linear filament(spL),loose spiraled filament(sps-L),regularly spiraled filament(sps-M) and tightly spiraled filament(sps-H),were respectively separated and axenically cultivated.Further research showed that there are significant differences in growth rate,photosynthesis,and content of chlorophyll a among four forms of filaments.Furthermore,SDS-PAGE analysis also showed some obvious differences in total proteins extracted respectively from four forms of Spirulina platensis.The 34 ku protein,which was highly expressed in the linear filaments,was almost absent from the helical filaments.国家自然科学基金(30571446)资

Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis

从钝顶螺旋藻SPIrulInA PlATEnSIS中分离纯化获得了螺旋形和直线形两种不同形态的藻丝体.通过对二者光合作用的研究发现,螺旋形藻丝体具有较高的光饱和光合作用速率(PMCHlA)和光饱和点,比直线形藻丝体更能适应较高光强的环境;而直线形藻丝体具有较低的光补偿点,能在更低的光强下进行光合作用.通过双向凝胶电泳对两种不同形态藻丝体的总蛋白进行比较分析,从中找出了9个差异表达的蛋白点.应用基质辅助激光解吸电离质谱对差异表达的蛋白质点进行鉴定和数据库检索,结果表明,3个蛋白与肽聚糖代谢有关,2个蛋白与光合作用有关,1个蛋白与细胞分裂调控相关,1个蛋白为外膜通道蛋白,2个蛋白为功能未知的假想蛋白.The two kinds of different morphological filaments(spiral and linear) of Spirulina platensis were separated and grown as axenic cultures.The photosynthetic characteristics show that compared with linear filaments,spiral filaments have higher light-saturated photosynthetic rate and light saturation point,and appeare to be more acclimated to high light intensity.Whereas linear filaments might be more acclimated to low light intensity for their lower light compensation point.To explore the morphologensis determination mechanism of Spirulina,the proteins that were differentially expressed in the spiral and the linear forms were studied.Two dimensional gel electrophoresis was performed to separate differentially expressed proteins that were subsequently identified by MALDI-Tof mass spectrometry.The 2-DE and PMF analysis revealed that at least nine proteins or subunits might relate to the morphogenesis of Spirulina platensis.The identified proteins include three proteins related to peptidoglycan metabolism,two photosynthetic proteins,one transmembrane channel protein,one protein related to cell division,as well as two unknown proteins.国家自然科学基金(批准号:30571446);福建省高等学校新世纪人才支持计划资

d<sup>0</Sup>過渡金屬化合物對氧氣反應性:合成和反應機理研究

眾所周知,過渡金屬如卟啉中的鐵與氧氣的結合和反應對許多生物功能和催化氧化至關重要.在這些反應中,過渡金屬一般含d價電子,并且金屬被氧化往往是其中一個重要的反應步驟.近年來,氧氣與d0過渡金屬化合物如Hf(NR2)4(R=烷基)的反應被廣泛用來制備金屬氧化物薄膜以作為新型微電子器件中的柵(門)絕緣材料.這篇專題文章討論我們近期對這些反應以及TiO2薄膜形成的研究.在許多氧氣與dn過渡金屬化合物的反應中,總是金屬被氧化.然而,在d0過渡金屬化合物如Hf(NMe2)4和Ta(NMe2)4(SiR3)與氧氣的反應中通常是配體被氧化.如—NMe2和—SiR3配體分別形成了—ONMe2和—OSiR3配體.反應機理和理論方面的研究顯示了微電子金屬氧化物薄膜形成的途徑

念青唐古拉山东南坡高寒草原生态系统表层土壤有机碳分布特征及影响因素

对念青唐古拉山东南坡高寒草原生态系统表层(0~20 cm)土壤有机碳分布特征进行研究,结果表明:有机碳密度平均为5.002 8±1.103 7 kg/m2,变异系数21.96%;在拔4421~4598 m内,随海拔升高表现增加→减少→增加的分布特征;与地上及10~20 cm土层生物量、20~30 cm含水量、土壤有机质、速效N、全N和全P含量呈显著正相关,与20~40 cm容重呈显著负相关。影响其的第1因子是植被盖度、地上生物量、20~30 cm地下生物量和20~30 cm含水量,第2因子是0~20 cm和20~40 cm容重及全P量,第3因子是有机质含量和速效N含量,第4因子是0~10 cm地下生物量,累计贡献率92.83%

西藏高寒草原生态系统表层土壤活性有机碳梯度分布及其与气候因子的关系

基于西藏高寒草原生态系统以水分为主要驱动力的东西样带和以温度为主要驱动力的南北样带内采集土样的实测数据,分析了表层(0~20cm)土壤活性有机碳的分布特征及其与气候因子之间的关系。结果表明,在28°46′-31°40′N的南北样带内,表层土壤活性有机碳含量随着纬度的增加而增加,当纬度增加到一定程度后,则随着纬度的增加而减少,呈现出南北低、中间高的分布特征。在80°02′-91°50′E的东西样带内,表层土壤活性有机碳含量随着经度的增加而增加,当经度增加到一定程度后,则随着经度的增加而减少,呈现出东西低、中间高的分布特征。影响南北样带内表层土壤活性有机碳分布的关键气候因子是年均气温,而影响东西样带内表层土壤活性有机碳分布的关键气候因子则是年均降水量