Gonad shafting hormone level quantitative analysis and clinical significance for the patients with premenopausal breast disease

目的:研究绝经前(滤泡期或称卵泡前期)的女性乳腺增生和乳腺癌患者HPO轴系激素表达,包括激素雌二醇(E2)、促卵泡激素(FSH)、促黄体激素(LH)、孕酮(P)、睾酮(T)和促催乳素(PRL)6种,为乳腺增生和乳腺癌提供诊断依据。方法:采用放射免疫法检测正常人、乳腺增生和乳腺癌患者各62例血浆E2、FSH、P、T和PRL水平(E2、P和T单位为μg/L,FSH和LH单位为I U/L,PRL单位为g/L),并应用逻辑回归与判别分析等方法进行统计分析。结果:乳腺癌、乳腺增生症和正常人3组人群的激素分布不同,乳腺癌患者的E2(192.59)、FSH水平增高(24.25),LH(30.235 3)与E2、FSH存在协同性增高,Logistic回归分析和聚类分析发现,T水平乳腺癌组低于乳腺增生与正常人群组,差异有统计学意义,P<0.05。P和PRL水平越高,患乳腺癌的危险度上升。结论:通过回归分析和聚类分析,可能提高临床应用性激素检测判别疾病的能力及危险度预测,判别准确率为91.2%。OBJECTIVE: To investigate the HPO shafting including E2,FSH,LH,P,T and PRL in the mammary gland hyperplasia and breast cancer of premenopausal(follicle period or prophase) female patients,and provide bases for mammary gland hyperplasia and breast cancer diagnosis.METHODS: By the radiative immune method,the blood levels of E2,FSH,LH,P,T and PRL(the unit of E2,P and T was μg/L,the unit of FSH and LH was IU/L,and the unit of PRL was g/L) were detected in 62 normal persons,62 patients with mammary gland hyperplasia and 62 patients with breast cancer.Logistic regression analysis and discriminance methods were used for the identification of the results.RESULTS: The hormone distributions in the mammary gland hyperplasia patients,breast cancer patients and normal people were diffrerent.By logical regression analysis and discriminance methods,the levels of E2(192.59) and FSH(24.25) in the breast cancer group increased,while LH(30.235 3) increased cooperativly with the increase of E2 and FSH.The level of T hormone in the breast cancer group was significantly lower than that in the mammary gland hyperplasia group and the normal people group,P<0.05.The higher of P and PRL levels were,the higher the breast cancer risk was.CONCLUSION:The use of logistic regression and distinguish analysis are favorable methods in the diagnosis of mammary gland hyperplasia and breast cancer.The total accuracy is 91.2%.厦门市科技社会发展计划基金(3502Z20044003

吡非尼酮药物缓释载体PTMAc-PEG-PTMAc水凝胶制备与体外实验

【目的】以PTMAc-PEG-PTMAc三嵌段（PPP）水凝胶为吡非尼酮（PFD）药物缓释载体，评价其药物缓释性能。观察PFD载药水凝胶对人眼Tenon′s囊成纤维细胞（HTF）增殖抑制作用。【方法】制备PPP水凝胶；研究其溶胀率及其影响因素；光学显微镜观察水凝胶（载药与空载）中人眼Tenon′s囊成纤维细胞（HTF）的形态学改变；直接接触法进行PPP载药水凝胶进行体外释药实验。通过测定浓度、计算累积药物释放量，绘制出两种水凝胶体外药物释放曲线。CCK-8法检测不同浓度不同时间点水凝胶材料对细胞活力的影响及载药水凝胶体外抑制HTF增殖。【结果】PPP水凝胶成胶稳定。扫描电镜显示水凝胶呈现三维立体网孔状结构。水凝胶具有溶胀特性。溶胀率随水凝胶材料浓度增加而减少。在体外释药试验显示在前4d有明显的药物突释，此后缓慢释药直至第14天达到平衡。增加载药浓度，减少凝胶体积将明显增加药物累积缓释量；显微镜观察PPP水凝胶有抑制HTF细胞黏附作用；PPP水凝胶与HTF相互作用24、48、72、96h观察细胞活力分别为85.7%、93.0%、82.0%、81.6%，水凝胶不同方位HTF细胞形态不尽相同；水凝胶对细胞有抑制细胞黏附作用。两组不同载药浓度（1mg/mL和2mg/mL）载药水凝胶在24、48、72、96h这4个时间点细胞增殖抑制率分别为25.8%、21.8%、55.4%、25.6%；44.6%、35.9%、55.5%、31.4%。而1mg/mLPFD溶液在24、48、72、96h这4个时间点细胞增殖抑制率分别为28.9%、29.7%，7.8%、7.7%。【结论】PPP水凝胶具备良好药物缓释性能，载吡非尼酮水凝胶能明显抑制HTF增殖且较含等量药物溶液长效

厦门海域水体无机氮和活性磷酸盐含量的变化趋势

根据2003～2008年每年丰水期、枯水期和平水期于厦门海域开展的海洋环境调查的资料,研究了该海域水体无机氮和活性磷酸盐含量区域分布和时间变化趋势.结果表明,调查期间该海域水体无机氮和活性磷酸盐平均含量都较高,分别为0.50、0.031 mg/dm3.其营养盐含量的区域分布相差较大,其中九龙江口水体无机氮含量最高,年均含量为0.52～1.37 mg/dm3;厦门西港水体活性磷酸盐含量最高,年均含量为0.039～0.061 mg/dm3;而大嶝海域水体无机氮和活性磷酸盐含量最低,年均含量分别为0.06～0.22、0.007～0.016 mg/dm3.调查期间全海域水体无机氮含量呈逐年增加趋势,活性磷酸盐含量在2003～2005年间呈上升趋势,而2005～2008年则有小幅度的下降.厦门海域水体N/P原子比较高,调查期间全海域年均值为27.4～47.5,且呈逐年增加趋势.无机氮含量的明显增加趋势及越来越严重的N/P比失衡,势必对该海域海洋生态系统尤其是浮游植物群落演替产生不良影响.此外,研究还发现厦门海域水体无机氮含量与盐度呈高度负相关(r=-0.96,n=30).这有力地证明了九龙江径流输入是厦门海域无机氮的最主要来源

Pollution assessment of heavy metals in the sediment of Jiulong River Estuary

选择九龙江河口水域的沉积物为对象,以地质累计指数法、生态危害指数法对该水域沉积物重金属的污染程度及潜在生态危害进行评价,并利用主成分分析法分析其可能的来源。地质累积指数结果表明,九龙江河口水域沉积物中zn、Cr均属无污染,大部分站位的Cu、Pb、Hg、AS为无污染或轻度污染,大部分站位的Cd则为轻度或中度污染;生态危害指数结果表明,九龙江河口水域沉积物重金属的潜在生态危害顺序为Cd>Hg>AS>Pb>Cu>Cr>zn;总体上九龙江河口水域的沉积物重金属污染属于中等生态危害;该水域重金属可能来源于有机质的降解以及工业排污和生活污水对沉积物的污染,其中前者为主要的可能来源。By the Index of Geoaccumulation and the Potential Ecological Risk Index,a pollution assessment of seven heavy metals(Hg,Pb,Cr,Zn,Cu,Cd,and As) was made of the sediment in Jiulong River Estuary.The results of pollution assessment by Index of Geoaccumulation revealed Zn and Cr in this area belonged to none pollutants while Cu,Pb,Hg,and As in most stations were non or light pollutants.However,Cd in most stations was assessed as light or moderate pollutant.The assessment result of ecological risk revealed this area belonged to moderate potential ecological risk.The order of ecological risk was as the following,Cd>Hg >As>Pb>Cu >Cr>Zn.Principal component analysis was applied to estimate the possible sources of heavy metal pollution in this area.Results showed that degradation of organic matter and industrial wastewater were the main sources of heavy metal contamination,but the former was more important than the latter.国家海洋局第三海洋研究所基本科研业务费项目(海三科2009023);国家海洋局公益性项目(200805064

Pollution characteristics and potential ecological risk assessment of heavy metals in the surface sediments of Tong'an Bay,Fujian,China

对厦门同安湾表层沉积物7种重金属(Hg、Cd、Pb、AS、Cu、zn和Cr)、有机碳和硫化物含量进行了相关性分析和因子分析,并应用沉积物质量评价标准及潜在生态危害指数法对重金属的潜在生态风险进行了评价.结果表明:Cu、Pb、zn、Cd、Cr、Hg、AS含量范围和均值分别为6.49~46.20,16.95;6.68~46.90,23.93;45.4~162.0,87.4;0.028 9~0.402 0,0.147 6;8.50~33.20,15.95;0.013~0.117,0.047和1.5~6.8,4.8Mg/kg.除AS之外,各重金属含量之间有显著的相关性(相关系数为0.666~0.970),且与有机碳含量的相关系数较高(相关系数为0.674~0.980).重金属元素及有机碳与硫化物含量的相关系数较低.潜在生态风险评价显示:该海区沉积物中重金属生物毒性效应频繁发生的几率相对较小,生态危害程度也较低,尚属轻微生态危害.7种重金属潜在生物毒性风险大小依次为:Hg>Cd>Pb>AS>Cu>zn>Cr.Concentrations of heavy metals,organic carbon and sulfides in surface sediments of Tong'an Bay were examined using correlation analysis and factor analysis.The potential ecological risk of heavy metals in Tong'an Bay was also assessed based on sediment quality assessment guidelines and an index of potential ecological risk.The results indicated that concentration of Cu ranged from 6.49mg/kg to 46.20mg/kg(mean: 16.95mg/kg);Pb ranged from 6.68mg/kg to 46.90mg/kg(mean: 23.93mg/kg);Zn ranged from 45.4mg/kg to 162.0mg/kg(mean: 87.4mg/kg);Cd ranged from 0.028 9mg/kg to 0.402 0mg/kg(mean: 0.147 6mg/kg);Cr ranged from 8.50mg/kg to 33.20 mg/kg(mean: 15.95mg/kg);Hg ranged from 0.013mg/kg to 0.117mg/kg(mean: 0.047mg/kg) and As ranged from 1.5mg/kg to 6.8mg/kg(mean: 4.8mg/kg).High positive linear correlations occurred among concentrations of heavy metals(r=0.666 to 0.970) except for As.High positive correlations also occurred between heavy metals and organic carbon(r=0.674 to 0.980).Concentrations of these heavy metals posed a low potential ecological risk and had a low probability of being associated with adverse biological effects.The sequence in the degree of their potential ecological risks was:Hg>Cd>Pb>As>Cu>Zn>Cr.国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(海三科2009062);福建省908专项资金资助项目(FJ908-02-02-02);集美大学科研基金资助项目(ZQ2007019

新疆棉花、小麦蚜虫生态治理技术

深入研究并充分利用农业害虫的自然控制因素，结合新疆农业系统中特有的自然生态条件特点，对害虫实施生态控制。发现麦双尾蚜的天敌100种，筛选出了低感虫品种、高感虫品种和耐害品种共15个，发现了具有潜在威胁的2个新物种，制订了以调整冬春麦种植结构和保护天敌为核心的麦双尾蚜自控防治技术和策略。可以利用苜蓿带自然繁育天敌可以用于控制棉蚜的生物学机理，即“苜蓿与棉花具有不同的主要昆虫种类，但具有相同的主要天敌类群”。提出了麦双尾蚜生物型演变成灾假说，提出“植物应该并且可以作为生物防治的重要因素加以利用”的观点，提出“相生植保”思路，创制新疆棉蚜生态控制技术体系，提出“新疆发展农区畜牧业、实现棉花害虫生态治理”应用模式

一种C16-C19长链正构烷烃脱氢催化剂及其制备方法和应用

本发明涉及一种用于C16-C19长链正构烷烃的脱氢催化剂及其制备方法和应用；催化剂组成按重量百分比：铂0.2～0.5％，锡0.4～2.0％，铁 0.1～2.0％，钾0.4～2.0％，其余为双孔分布的大孔径条状氧化铝；用于长链正构烷烃C16-C19脱氢制取单烯烃反应，转化率11.4％，选择性91.9％，可长时间地进行C16-C19催化脱氢反应，脱氢产物合成的烷基苯磺酸盐对于实际油田油水可以在较宽的碱浓度范围内达到超低界面张力，与 C10-C13烷基苯副产物重烷基苯磺酸盐性能驱油相比，碱浓度向低浓度方向偏移，样品界面活性更好。带填

CRU 数据集在黑龙江省 ET0计算中的应用/Application of CRU dataset to calculation of ET 0 of Heilongjiang Province[J]

针对我国参考作物蒸发蒸腾量(ET 0)计算中存在的气象数据不易获取、现有作物需水量数据空间分辨率较低等问题，以黑龙江省为例，基于10 min 分辨率的 CRU CL 2.0数据集，采用 FAO Penman-Monteith 公式建立了适于计算区域 ET0的栅格模型，并应用黑龙江省14个气象站的实测气象数据对该模型计算成果进行了验证。结果表明：采用 CRU CL 2.0数据计算区域 ET0是可行的，其计算结果与应用实测气象数据计算所得的 ET 0符合较好，不仅可以细化我国 ET0和作物需水量研究成果，也为气象资料缺乏情况下的 ET 0计算供了一种新的计算模式