Identification and Determination of Absorbed Components of Danggui-Shaoyao-San in Rat Plasma

Aim: To investigate the absorbed components of Danggui-Shaoyao-San (DSS) in rat plasma and their pharmacokinetics. Methods: Serum samples from rats with DSS extract administrated orally were analyzed by HPLC-DAD-MSn and an HPLC-DAD method was established for the determination. Results: Seven prototypes and one metabolite were identified in rat serum, and albiflorin, paeoniflorin, ferulic acid and ligustilide in rat plasma were determined for pharmacokinetic study. The proposed method was validated with a linear range of 0.025-5.60 μg·mL-1 and limit of quantitation (LOQ) at 0.022-0.180 μg·mL-1, respectively. The RSD of intra- and inter-day precision variations were less than 10.1% and the accuracies ranged from 92.5%-111.5%. The overall recovery was over 80%. Conclusion: Paeoniflorin sulfonate, albiflorin, paeoniflorin, ferulic acid, senkyunolide I, ligustilide and butylidenephthalide were found to be the potentially effective constituents in DSS. The method for determination is simple and sensitive, which is proved to be suitable for pharmacokinetic study of albiflorin, paeoniflorin, ferulic acid and ligustilide in DSS. © 2011 China Pharmaceutical University.目的:研究大鼠口服給藥當歸芍藥散后體內的入血成分及其藥代動力學。方法:大鼠灌胃給予當歸芍藥散提取物后,以HPLC-DAD-MSn分析含藥血清中藥物成分,并建立主要入血成分的HPLC測定方法以應用于藥代動力學研究。結果:大鼠灌胃給予當歸芍藥散提取物后血清中檢測到7個原型成分和1個代謝產物,并對大鼠血漿中芍藥內酯苷、芍藥苷、阿魏酸和藁本內酯進行了測定。方法學考察了線性范圍(0.025-5.60μg·ml-1)、定量限(0.022-0.18μg·ml-1)、日內和日間精密度(80%)。結論:芍藥苷硫酸酯、芍藥內酯苷、芍藥苷、阿魏酸、川芎內酯I、藁本內酯和丁烯基苯酞為當歸芍藥散主要的入血成分。對大鼠血漿中芍藥內酯苷、芍藥苷、阿魏酸和藁本內酯建立的測定方法簡便、準確、靈敏度高,適用于血藥濃度測定及其藥動學研究

MCP-1/CCR2通路参与骨髓基质细胞向脊髓全横断损伤区迁移的实验研究

【目的】 探讨单核细胞趋化蛋白-1（MCP-1）及其受体CCR2在骨髓基质细胞（MSC）体内、外迁移中的作用。【方法】体外培养、纯化大鼠MSC，取第五代MSC行免疫荧光鉴定；免疫组化、RT-PCR检测纯化MSC表达CCR2情况；Boyden 小室法检测趋化因子MCP-1（5～500 ng/mL）对MSC的趋化迁移作用及其特异性。42只成年大鼠用于体内研究（脊髓全横断组24只，假手术组9只，正常大鼠9只），分别于术后1，3，7，14d取材，行免疫组化检测MCP-1表达，或行MCP-1的Real-time PCR定量分析，或颈内静脉注射荧光标记的MSC，观察MSC向脊髓迁移情况。【结果】第五代MSC都表达间充质干细胞标记物Vimentin、Laminin及Fibronectin；细胞免疫荧光、RT-PCR证实MSC表达趋化因子受体CCR2；MCP-1（5～50 ng/mL）体外可趋化MSC迁移（P﹤0.05），抗MCP-1抗体可对抗其趋化迁移作用（P﹤0.05）。脊髓全横断组、对照组脊髓均表达MCP-1，但细胞分布、染色存在差异，影响MCP-1定量。脊髓损伤后趋化因子MCP-1RNA在1d、3d及7d较对正常对照组差异有统计学意义（P <0.05），术后14d时MCP-1RNA与正常对照相比差异无统计学意义（P ＞0.05），伴随其变化，脊髓损伤区迁移MSC较对照差异有统计学意义（P﹤0.05）。【结论】 MCP-1体内、外可趋化MSC迁移，MCP-1/CCR2通路参与MSC向脊髓全横断损伤区的迁移

一種用於進行開環聚合反應的催化劑及其製法

本發明係關於一種可催化化學開環聚合(Ring Opening Polymerization, ROP)反應之催化劑，特別是關於一種具有鎂的芳氧化合物結構的催化劑及其製造方法，以及此種催化劑應用於開環聚合反應的方法。此鎂的芳氧化合物無毒性，不易殘留於聚合反應所生成的聚合物中，可提高聚合物產品的安全性。此外，此種催化劑對於開環聚合反應有極佳之催化效果，與習知的同類型反應而言，使用此催化劑進行開環聚合反應，具有反應溫度低，反應時間短，轉化率高，以及產率高等優點

Surveillance on Turkey, Ostrich and Waterfowl Avian Influenza

對水禽類動物鴨、鵝.雞及火雞共計265場，6000支肛門拭子或血清分別以應用反轉錄聚合鋂鏈進行檢測病毒核酸，而血清則以免疫酵素分析法及AGP等方法進行禽流感抗體之監測，如為陽性反應之檢體病進行亞型之鑑定，陽性率過高之場別，擬進行現場採集檢體進行病毒分離及以應用反轉錄聚合鋂鏈進行檢測，以早期發現未曾在台灣發生者，或者在台灣已成為地方性疾病，但有爆發成流行之趨勢者。透過台灣區人工飼養鴕鳥協會及專業獸醫師，進行鴕鳥之死亡及飼養失敗病例之收集。並進行病理之解剖、病因之探討及病原分離與鑑定。並就環境、病理學、病原所得之資料進行分析，提出解決之建議方案。Sera samples will be collected seasonally from all over the country. Avian influenza antibody of ELISA, agar gel precipitation (AGP), and hemagglutination inhibition (HI) test. Fecal samples will be collected from the antibody positive flocks, and then subjected to RT-PCR for AIV detection and subserotype determination. Domestic turkeys will detects for serological antiboies to avian influenza virus in Taiwan, sampling 20 from each group or farm to testing with enzyme-linked immunosorbent assay (ELISA). The antibody prevalence will be analyzing as a data for prevention of AI. The turkey farms showed positive reaction after serum detection will be test to viral isolation and sub-serotyping, this farm will going to sera monitoring. In this project, we will cooperate with Taiwan Domestic Ostrich Association and employ a professional veterinarian to propagate the important of monitoring disease. We will collect clinical case ofostrichs and analysis the aetiology to establish the disease database of ostrich in Taiwan

中国被子植物濒危等级的评估

本文基于文献和标本信息收集以及专家提供的数据,运用IUCN濒危物种红色名录方法首次对中国范围内所有已知被子植物进行灭绝风险评估。结果显示,在评估的30,068种被子植物中,灭绝等级(含灭绝、野外灭绝、地区灭绝)共计40种;受威胁等级(极危、濒危、易危)3,363种,受威胁比例为11.18%。从空间分布看,我国受威胁被子植物主要集中分布在西南地区以及台湾、海南等岛屿,且主要分布在中低海拔地区。对受威胁物种的分析结果表明,包括原生植被破坏在内的生境丧失及破碎化是我国被子植物濒危的首要因子,涉及约84.1%的受威胁物种;过度采挖和物种内在系统问题位列致危因子的第二、三位,分别涉及38%和14%的物种。其他的致危因子包括外来入侵种在内的种间竞争、环境污染、自然灾害和全球气候变化等。一个物种的致危因子往往是多方面的。本次评估与2004年红色名录相比,生境变化、实施保护措施及分类学新修订使一些物种的濒危等级发生了变化,这也印证了红色名录是一个动态的系统,需要根据最新信息进行更新,以便为生物多样性保护提供实时准确的基础数据

中国高等植物受威胁物种名录

2008年,环境保护部和中国科学院联合启动了《中国生物多样性红色名录——高等植物卷》的编制工作。通过这项工作,我们依据IUCN濒危物种红色名录标准对中国野生高等植物的濒危状况进行了全面评估,编制了中国高等植物红色名录。2013年9月,该名录以环境保护部、中国科学院第54号公告形式发布,即《中国生物多样性红色名录—

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^