油酰乙醇胺在小鼠急性局灶性脑缺血再灌注损伤中的作用及机制

目的研究油酰乙醇胺(OEA)在脑缺血再灌注损伤中的作用及机制。方法线栓法制备小鼠大脑中动脉栓塞模型,缺血90 min后再灌注。应用HPLC-MS/MS方法测定脑组织内OEA的含量。给予OEA(5,10,40 mg/kg,ig)或OEA水解酶抑制剂URB597(1 mg/kg,ig),观察其对小鼠急性脑缺血再灌注损伤的影响。测定脑组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)及过氧化氢酶(CAT)的活性。观察MK886对OEA抗脂质过氧化损伤的影响。结果脑缺血再灌注后6 h,损伤侧脑内OEA含量开始升高,再灌注后24 h升高最明显。脑缺血再灌注后给予OEA(40 mg/kg)或URB597(1 mg/kg)可减少神经功能缺失评分,减小脑梗死体积,减轻脑水肿程度。OEA可减少脑内MDA含量,增加抗氧化酶SOD的活性。OEA这一抗氧化作用可被MK886所取消。结论脑缺血再灌注可增加脑内OEA的含量,OEA通过激动PPARα,减轻脂质过氧化损伤发挥抗脑缺血再灌注损伤作用

Clinical Observation on Kangshi Kangxian Formula Combined with Interferon and Ribavirin in 33 Cases of Chronic Hepatitis C

目的观察康氏抗纤方联合聚乙二醇化干扰素α-2A(PEg-Ifnα-2A)、利巴韦林对慢性丙型肝炎(CHC)患者抗病毒治疗应答及生活质量的影响。方法选择CHC患者64例,随机分为治疗组33例与对照组31例。对照组给予PEg-Ifnα-2A注射液180μg/次,皮下注射,每周1次;利巴韦林0.3 g/次,口服,每天3次。治疗组在对照组基础上口服康氏抗纤方,每日1剂。两组均治疗24周。观察两组患者治疗前后肝组织病理、肝纤维化瞬时弹性测定值、血清丙氨酸氨基转氨酶(AlT)水平、丙肝病毒定量(HCV rnA)及慢性肝病问卷(CldQ)积分的变化。结果两组患者病毒学应答与生化应答比较差异无统计学意义(P>0.05)。治疗后两组肝脏炎症及纤维化程度均较本组治疗前显著改善(P 0.05).Inflammation and fibrosis of the liver were significantly improved after treatment( P < 0.05) compared within each group before.Improvement of liver fibrosis in treatment group is greater than control group( P < 0.05).On the improvement of Fibro Scan value,treatment group was obviously better than control group( P < 0.05).Every aspect of CLDQ scores of treatment group had different levels of increases( P < 0.01),while control group only had some increases on systemic symptom,activity ability and emotion scores( P < 0.05).After treatment,treatment group' s CLDQ scores on abdomenal pain,sleepinese,emotion and anxiety were notedly better than contreol group( P < 0.01).Conclusion Using Kangshi Kangxian Formula combined with PEG-IFNα-2a and ribavirin to treat CHC not only increased patient's response to antiviral therapy,but also improve the condition of liver fibrosis and patient's quality of life.福建省卫生厅青年科研课题基金资助项目(2012-2-105

粘结界面力学行为及其表征

胶接技术具有工艺简单、应力集中小、抗疲劳、耐腐蚀、密封性好等优点,在机械、建筑、电子、航空航天、医疗等诸多领域中广泛应用,但存在粘结强度相对较弱、界面强度影响因素较多等问题,故针对粘结界面力学行为及其表征技术开展研究具有重要的学术意义和应用价值.本文首先介绍粘结界面的破坏机理以及影响因素的相关研究,侧重于有关粘结界面强度和应力分析的理论和实验工作,然后针对粘结层存在一定程度的尺度依赖的现象,总结国内外在粘结层尺度效应方面的研究现状,最后介绍粘结界面在制备及服役期间可能产生的各类缺陷及其对粘结构件整体力学行为的影响

Clinical Observation on 35 Cases of Chronic Hepatitis B Complicated with Depression Treated with Changyu Xiaoyao Fang( 菖郁逍遥方) Combined with Entecavir Dispersible Tablets

目的观察菖郁逍遥方联合恩替卡韦分散片治疗慢性乙型病毒性肝炎伴抑郁症的临床疗效及可能的作用机制。方法 67例慢性乙型病毒性肝炎伴抑郁症患者随机分为治疗组35例、对照组32例。在口服恩替卡韦分散片每日0.5 Mg的基础上,对照组加服氟哌噻吨美利曲辛片,每日2次,每次1片;治疗组加服中药复方菖郁逍遥方,每日1剂,两组疗程均为8周。治疗前后分别观察两组患者17项汉密尔顿抑郁量表(HAMd)、慢性肝病问卷(CldQ)积分以及血清白细胞介素6(Il-6)、肿瘤坏死因子α(Tnf-α)水平;比较两组患者抑郁状态疗效、血清丙氨酸氨基转移酶(AlT)复常率及乙型肝炎病毒dnA(HbV dnA)阴转率。结果治疗组抑郁状态疗效总有效率、AlT复常率、HbV dnA阴转率分别为80.00%、80.00%、60.00%,对照组分别为65.63%、62.50%、46.88%,治疗组均优于对照组(P<0.05)。与治疗前比较,治疗后两组患者HAMd评分、Il-6、Tnf-α水平均降低,CldQ各项得分及总积分均增加(P<0.05或P<0.01);治疗后治疗组Il-6、Tnf-α水平和CldQ各项得分及总积分改善程度较对照组更显著(P<0.05)。结论菖郁逍遥方联合恩替卡韦分散片可显著改善慢性乙型病毒性肝炎伴抑郁症患者的抑郁状态,并能增强抗病毒疗效、提高患者生活质量,其作用机制可能与抑制炎症反应相关。Objective To observe the clinical effect and possible mechanism of Changyu Xiaoyao Fang combined with entecavir dispersible tablets in treating chronic hepatitis B accompanied with depression.Methods67 patients with chronic hepatitis B accompanied with depression were randomly divided into a treatment group of 35 cases and a control group of 32 cases.In addition to taking 0.5 mg of entecavir dispersible tablet orally,control group was given flupentixol( 0.5 mg) and melitracen( 10 mg) tablet,one tablet twice a day orally,and treatment group was given Changyu Xiaoyao Fang one dose a day orally,both for 8 weeks.The scores of Hamilton Depression Scale( HAMD) and Chronic Liver Disease Questionnaire( CLDQ),as well as serum levels of interleukin 6( IL-6) and Tumor Necrosis Factorα( TNF-α) were detected before and after treatment.The improvement of depression,the normalization rate of serum level of alanine aminotransferase( ALT),and negative conversion rate of Hepatitis B Virus DNA( HBV DNA) in patients in both groups were observed after treatment.Results After treatment,the total effective rate in depression improvement,the normalization rate of ALT,and the negative conversion rate of HBV DNA was 80.0%,80.00% and 60.00%,respectively in treatment group,65.6%,62.50% and 46.88%,respectively in control group.The effects of all indexes in treatment group were superior to those in control group( P <0.05).The scores of HAMD,and levels of IL-6 and TNF-αwere decreased,and the scores of every items and total score of CLDQ were increased after treatment( P < 0.05 or P < 0.01).The improvement of IL-6,TNF-αand the scores of every items and total score of CLDQ in treatment group were superior to those in control group( P < 0.05).Conclusion Changyu Xiaoyao Fang combined with entecavir dispersible tablets could improve depression condition in patients with chronic hepatitis B accompanied with depression,strengthen antiviral effect,and improve quality of life.The mechanism may relate to inhibiting inflammation reaction.福建省卫生厅青年科研课题资助计划(2012-2-105); 福建省自然科学基金(2014J01374

一例华南虎纤维组织瘤样增生的诊治

韶关某繁育基地一只14岁雄性华南虎（Panthera tigris amoyensis）右前脚掌背皮肤出现破损红肿，且肿块日渐增大。专家初步诊断该肿块为良性肿瘤，并实施切除手术。通过血常规、生化指标检查、分子生物学以及病理组织学技术进行检查与诊断分析。结果显示：患虎血细胞比容、平均红细胞体积、平均红细胞血红蛋白含量3项指标降低，丙氨酸氨基转移酶和天冬氨酸转氨酶显著升高且存在立克次氏体感染。此外，病理组织学鉴定切除肿块为纤维组织瘤样增生，为良性肿瘤。这一病例报导，可为虎此类疾病的诊断及防控提供经验

云南玉溪绿孔雀遗传多样性分析:与圈养种群的比较

绿孔雀(Pavo muticus)是备受关注的濒危物种。我国南方曾广泛分布的云南亚种(P.m.imperator)目前收缩到中部、南部和西部的局部地区,近年来陆续开展了野外种群调查,但遗传多样性的分析和评估尚未开展。本研究利用从玉溪市新平和峨山交界处采集的野外绿孔雀自然脱落的羽毛,采用11对微卫星标记分析遗传多样性和结构,并与国内主要圈养种群(建群者来自瑞丽地区和其他未确定的地点)进行比较。结果表明:该野生种群的遗传多样性较低,受到近交的影响,遗传多样性进一步丢失的风险较高。遗传结构上,该野生种群和圈养种群的个体被划分为3个亚群,野生种群作为独立的1个亚群,在遗传上有别于圈养种群所含的2个遗传谱系,说明云南省分布的绿孔雀有着显著而复杂的地理分化。当前需要开展系统的谱系地理学研究,以加强族群之间的基因交流为目标,通过生态廊道或个体转移等方式增加遗传交流的机会,扭转遗传多样性随近交的深化而不断丢失的趋势