Expressions of Epstein-Barr Virus Latent Membrane Lmpl and P53 Protein in Nasonpharyngeal Carcinoma and their Clinical Significance

目的　探讨 EB病毒潜伏感染和抑癌基因 P5 3异常表达与鼻咽癌的关系。方法　用免疫组化 S- P方法对 5 9例鼻咽癌 (NPC)和 12例鼻咽部正常或慢性炎症组织进行单克隆抗体 L MP1、P5 3标记 ,并结合组织学类型、分化程度、发病年龄进行分组研究。结果　正常和慢性炎症的鼻咽粘膜均呈阴性表达。NPC中 ,L MP1阳性率 6 9.4 9%(4 1/ 5 9) ,P5 3阳性率 4 4 .0 6 % (2 6 / 5 9) ,双阳性率 30 .5 1% (18/ 5 9)。L MP1在非角化性癌、低分化癌及青年组 NPC中高表达 ;在鳞癌、高分化癌及老年组中低表达。P5 3在低分化癌中高表达 ;但与 NPC组织学类型、发病年龄不存在明显相关性。结论　在 NPC发生、发展过程中 EBV潜伏感染起主导作用 ,P5 3基因异常表达可能起协同作用。EB病毒潜伏感染是 NPC发病年龄年轻化的主要原因之一Objective To study the correlation of Epstein Barr Virus Latent infection and suppressor gene P53 mutation with the human nasonpharyngeal Carcinoma(NPC).Methods\ The nasonpharyngeal bioptic tissue of 59 cases of NPC and 12 cases of normal and chronic inflammation were examined to observe the Lmp 1 and P53 protein expression by using immunohistochemistry technique(S P methods).Results\ In 59 cases of NPC,Lmp 1 positive rate is 69 49%,P53 44 06% and both P53 and Lmp 1 30 51%.Statistically positive expression of Lmpl was correlated with hostologica type and differentiation degree of NPC,and age of illness.Statistically positive expression of P53 was correlated with low differentiation tumor.There are no significant differences in positive expression of P53 among various ages and hostological type.Conclusions In the course of tumorigenesis of NPC,Epstein Barr Virus latent infection plays the major role and P53 mutation plays the coordination role.Epstein Barr Virus latent infection is the main course that young people get ill