网络环境下电子出版物的分编和管理对策

电子出版物的出现与发展,改变了图书馆馆藏信息资源结构,使得图书馆传统的以纸本文献为主体的编目及管理工作发生深刻变化。文章试从图书馆实际工作出发,对这一变革中出现的新问题进行积极地探讨并提出对策

Electrochemical Nucleation of Au on n-Type Semiconductor Silicon Electrode Surface

在成功实现半导体硅表面电沉积致密金膜的柠檬酸盐镀金实际应用体系中,运用循环伏安和电位阶跃法研究了Au在n型SI(111)电极表面的电沉积过程和成核机理.结果表明,在该体系中,Au在SI表面呈现不可逆电极过程,成核过电位达到250 M V;根据COTTrEll方程求得扩散系数d=(1.81±0.14)x10–4 CM2 S–1;运用SCHArIfkEr-HIllS(SH)理论模型对比分析拟合实验结果,表明Au在n型SI表面遵循扩散控制下的三维连续成核机理;通过扫描电子显微镜观察Au初期成核、生长形貌,进一步证实了Au的三维连续成核机制,并讨论了阶跃电位和阶跃时间对Au核形貌和密度的影响.Cyclic voltammetry and chronoamperometry have been used to investigate the mechanism of gold electrodeposition on the n-Si(111) electrode surface from a citrate bath, which had successfully applied to directly electroplate a dense gold film on the silicon surface.The results show that Au electrodeposition on the n-type silicon surface is an irreversible process, and the nucleation overpotential reaches 250 m V.According to Cottrell equation, the diffusion coefficient(D) is calculated to be(1.81 ± 0.14) × 10-4 cm2 s-1.The Scharifker-Hills(SH) model was used to analyze the experimental data.Agreement between the fitting curves and the theoretical curves confirms that the nucleation process of Au electrodeposition on the n-type silicon surface follows the progressive nucleation mechanism with three-dimensional growth under diffusion control.To further confirm the progressive nucleation mechanism, scanning electron microscopy(SEM) was used to observe the nucleation and growth of Au deposits at the initial stage of electrodeposition.The SEM results show that the morphology and density of the Au deposits are affected by the electrochemical deposition potential and time.国家重大科学仪器设备开发专项(2011YQ03012406); 国家自然科学基金界面电化学创新群体(21321062)及国家自然科学基金(21373172)资助项目~

Specific Release of Bacteriochlorophylls B800 of LH2 from Rhodobacter azotoformans Induced by Sodium Dodecyl Sulfate

采用吸收光谱法研究了十二烷基硫酸钠(SdS)诱导rHOdObACTEr AzOTOfOrMAnS外周捕光复合体lH2细菌叶绿素(bACTErIOCHlOrOPHyllS,bCHlS)的解离行为和规律.结果表明:室温下,在10MMOl？l-1TrIS-HCl(PH8.0)缓冲液中,低浓度SdS只诱导lH2中b800细菌叶绿素解离生成游离bCHlS,而b850不受影响;当浓度达到0.08%(W/V)时,能特异性地诱导b800缺失,其缺失过程和游离bCHlS的生成过程均符合单指数模型,且二者的速率常数近似相等.高浓度SdS能同时诱导b800和b850解离生成游离bCHlS,其中b800可发生缺失,而b850则不完全解离,解离过程均符合单指数模型;b800对SdS更敏感,其解离速率常数约是b850的4倍,游离bCHlS生成速率常数明显低于b800解离速率常数,而与b850解离速率常数相接近.The release behaviors of bacteriochlorophylls of peripheral light-harvesting complex LH2 from Rhodobacter azotoformans induced by sodium dodecyl sulfate (SDS) were investigated using absorption spectroscopy.The results indicated that bacteriochlorophylls of B800 band are released from their binding sites and transformed into free bacteriochlorophylls by incubating LH2 sample in 10 mmol?L-1 Tris-HCl (pH 8.0) buffer containing SDS of low concentration at room temperature.However, the bacteriochlorophylls of B850 band are not released.The dynamics of B800 release and free BChl formation induced by 0.08% (w/V) SDS can be well fitted by the monoexponential model.The rate constant of B800 release is nearly equal to that of free BChls formation.The release of both B800 and B850 of LH2 can be induced by high concentration SDS, simultaneously.The bacteriochlorophylls of B800 band can be completely transformed into free BChls, but not for B850.Although both of their release processes show monoexponential models in 1% SDS solution, the release rate constant of B850 is remarkably lower than that of B800 and close to that of free BChls formation.国家自然科学基金(No.30970068);国家科技基础条件平台建设(No.2005DKA21209);厦门大学近海海洋环境科学国家重点实验室高级访问学者基金(No.MELRS0907);山西省回国留学人员科研(No.200713)资助项

塞克硝唑片治疗滴虫阴道炎的随机对照多中心临床研究

【目的】评价口服塞克硝唑片治疗滴虫阴道炎的有效性和安全性。【方法】采用多中心、随机双盲、平行对照设计，入选滴虫阴道炎患者144例，塞克硝唑片组与替硝唑片组各72例，对其临床疗效和滴虫清除率进行比较，并观察用药后不良事件和实验室检查异常的发生情况。【结果】用药后塞克硝唑片组痊愈率和有效率分别为61％和94％；替硝唑片组痊愈率和有效率分别为51％和94％，两组之间比较差异无统计学意义（P〉0．05）。塞克硝唑片组滴虫清除率为96％；替硝唑片组滴虫清除率为97％，两组比较差异无统计学意义（P〉0．05）。144例病例中无不良事件与严重不良事件发生；塞克硝唑片组实验室检查异常发生率为0，替硝唑片组实验室检查异常发生率为4％，两组之间比较差异无统计学意义（P〉0．05）。【结论】口服塞克硝唑片治疗滴虫阴道炎安全、有效

原位心脏移植（7例报告）

【目的】总结7例心脏移植经验，探讨心脏移植的近远期疗效。【方法】1998年10月至2005年4月施行7例原位心脏移植，7例均为心肌病，其中5例为扩张型，2例为限制型，手术方法采用标准法4例、双腔静脉法3例，供心平均冷缺血时间为（167．4±22．1）min，术后定期行心内膜活检，使用四联免疫抑制剂。【结果】第1例存活5d，死于低心排及主动脉内球囊反搏（IABP）的并发症，第4例存活18月，死于中一重度的急、慢性并存的排斥反应，余5例至今存活，至今存活时间分别为6年9个月、5年6个月、1年7个月、10个月、3个月。【结论】心脏移植是治疗终末期心脏病的有效手段，处理好术后并发症，密切监测和处理排斥反应，能取得良好的近远期疗效，长期生存病例需特别注意慢性排斥反应的发生

Analyze the epidemical multidrug-resistant Mycobacterium tuberculosis from Shenzhen by DNA sequencing

通讯作者: 杨慧( yh2009cn@y ahoo.com.cn)[中文文摘]目的了解深圳市耐多药结核分枝杆菌(multidrug-resistant Mycobacterium tuberculosis,MDR-TB)的分子流行病学特征。方法参照WHO/I UATLD标准,使用L-J药敏培养基,采用1%比例法药敏试验筛选出敏感株和异烟肼(isoniazid,INH)、利福平(rifampicin,RFP)双耐药临床分离株,通过DNA测序检测深圳地区153株敏感株与132株MDR-TB的INH耐药基因katG、inhA、oxyR-ahpC基因间区域及RFP耐药基因rpoB碱基排列顺序,运用DNASTAR和blastn进行序列分析。结果 153株敏感株突变率为27.5%(42/153)。132株MDR-TB突变率为98.5%(130/132),其中katG基因突变率为73.5%(97/132),68.9%(91/132)为katG315位突变;inhA基因突变率为18.2%(24/132),11.4%(15/132)为启动子区域突变,未发现inhA94特异突变株;ahpC基因突变率为16.7%(22/132),10.6%(14/132)为启动子区域突变;rpoB基因81 bp核心区域突变率为93.2%(123/132)。结论 katG315、inhA启动子区域、ahpC启动子区域以及rpoB 81 bp核心区域突变为深圳地区耐多药结核分枝杆菌主要突变类型,与其他国家和地区差异无统计学意义;但深圳地区未见inhA94突变株。[英文文摘]Objective To investigate the epidemiologic characteristic of mult idrug-resistant (MDR) Mycobacterium tuberculosis (MTB) in Shenzhen. Methods According to the standard of WHO, International Union Against Tuberculosis and Lung Disease (IU ATLD) ,drug-susceptive and MDR MTB strains were collected by drug suscept ibility test (DST) of 1% proportion method.kat G, inh A, intergenic region of oxy R-ahp C and 81bp core region of rpo B genes in 153 drug-sus-ceptive strains and 132 MDR strains were analyzed by DNA sequencing. Results 27.5%(42/153) of drug-susceptive strains and 98.5% (130/132) of MDR strains showed gene mutat ions. Of 132 MDR strains, 73.5%( 97/132) had ka t G mutat ions, and 68.9% (91/132) of kat G mutations were at codon 315; 18.2% (24/132) had in h A mutations, and none specific mutations were found at codon 94 of in h A; 11.4% (15/132) had mutations at inh A-promoter region; 16.7% (22/132) had ahp C mutat ions, 10.6% (14/132) had mutations at interg enic region of oxyR-ah p C; 93.2%( 123/132) had mutations at the 81bp core region of rpoB. Conclusion The codon 315 of kat G, promoter region of inh A, ahp C and the 81bp core region of rpoB play predominant roles in MDR-T B in Shenzhen.艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2008ZX10003-004