Role of Kv1. 3 channel of CD4--+ T lymphocytes in the formation of atherosclerosis in rat spleen

目的:探讨大鼠动脉粥样硬化(AS)模型中Cd4+T淋巴细胞电压门控钾通道(kV)kV1.3的表达、功能及其在AS中的作用。方法:采用高脂饮食法建立大鼠动脉粥样硬化模型,流式细胞术分析淋巴细胞的比例,采用免疫磁珠法分离Cd4+T淋巴细胞,研究脾组织Cd4+T淋巴细胞kV1.3MrnA表达、细胞内钙离子浓度及细胞因子分泌的变化。结果:(1)AS组脾组织Cd4+T淋巴细胞占总T淋巴细胞比例较对照组明显升高(74.93%±2.15%VS67.80%±2.54%,P<0.05)。(2)经刀豆蛋白A(COnA)刺激,AS组T淋巴细胞增殖程度明显高于对照组(1.1321±0.1750VS0.7971±0.0955,P<0.05)。(3)AS组脾组织Cd4+T淋巴细胞在COnA刺激状态下胞内钙离子浓度明显高于对照组(H=82,P<0.05)。(4)AS组脾组织Cd4+T淋巴细胞在刺激48H后较刺激24H后细胞因子(Il-2,Tnf-α)分泌显著增加。(5)AS组脾组织Cd4+T淋巴细胞kV1.3MrnA表达明显高于对照组(3.670±1.579VS1)。结论:AS组脾组织Cd4+T淋巴细胞比例高于对照组,Cd4+T淋巴细胞kV1.3MrnA表达增多,提示高表达kV1.3的Cd4+T淋巴细胞可能在AS的发生发展中发挥重要的作用。AIM: To investigate the function of voltage-gated potassium channel Kv1.3 and its possible role in CD4 + T lymphocytes in the formation of atherosclerosis ( AS) in rat spleen.METHODS: The rat atherosclerosis model was established by feeding high-fat diet.The proportion of lymphocytes was determined by flow cytometry.The CD4 + T lymphocytes were separated using immunomagnetic bead.The mRNA expression of Kv1.3 in CD4 + T lymphocytes was detected.The concentrations of intracellular calcium and cytokines were also measured.RESULTS: ( 1) The proportion of CD4 + T lymphocytes in AS group was significantly higher than that in control group ( 74.93% ± 2.15% vs 67.80% ± 2.54% ,P < 0.05) .( 2) After stimulated with concanavalin A ( ConA) ,the proliferation of CD4 + T lymphocytes in AS group was significantly higher than that in control group ( 1.1321 ± 0.1750 vs 0.7971 ± 0.0955,P < 0.05) .( 3) After stimulated with ConA,the concentration of intracellular calcium in AS group was higher than that in control group.( 4) In AS group,the releases of cytokines of IL-2 and TNF-α in AS group were significantly higher when stimulated with ConA for 48 h than that for 24 h.( 5) The mRNA expression of Kv1.3 in CD4 + T lymphocytes was greatly higher in AS group than that in control group ( 3.670 ± 1.579 vs 1) .CONCLUSION: In AS rats,the increase in CD4 + T lymphocytes as well as the augmentation of Kv1.3 mRNA expression in the cells suggest that up-regulation of Kv1.3 mRNA expression in CD4 + T lymphocytes may be involved in the mechanism of atherosclerotic formation in rat spleen.国家自然科学基金资助项目(No30871045

CD4~+T淋巴细胞Kv1.3通道在大鼠动脉粥样硬化中的作用

目的:探讨大鼠动脉粥样硬化(AS)模型中CD4+T淋巴细胞电压门控钾通道(Kv)Kv1.3的表达、功能及其在AS中的作用。方法:采用高脂饮食法建立大鼠动脉粥样硬化模型,流式细胞术分析淋巴细胞的比例,采用免疫磁珠法分离CD4+T淋巴细胞,研究脾组织CD4+T淋巴细胞Kv1.3mRNA表达、细胞内钙离子浓度及细胞因子分泌的变化。结果:(1)AS组脾组织CD4+T淋巴细胞占总T淋巴细胞比例较对照组明显升高(74.93%±2.15%vs67.80%±2.54%,P<0.05)。(2)经刀豆蛋白A(ConA)刺激,AS组T淋巴细胞增殖程度明显高于对照组(1.1321±0.1750vs0.7971±0.0955,P<0.05)。(3)AS组脾组织CD4+T淋巴细胞在ConA刺激状态下胞内钙离子浓度明显高于对照组(H=82,P<0.05)。(4)AS组脾组织CD4+T淋巴细胞在刺激48h后较刺激24h后细胞因子(IL-2,TNF-α)分泌显著增加。(5)AS组脾组织CD4+T淋巴细胞Kv1.3mRNA表达明显高于对照组(3.670±1.579vs1)。结论:AS组脾组织CD4+T淋巴细胞比例高于对照组,CD4+T淋巴细胞Kv1.3mRNA表达增多,提示高表达Kv1.3的CD4+T淋巴细胞可能在AS的发生发展中发挥重要的作用

Effects of Heavy Metal and Pollutants on the Non-special Immunity of the Shrimp and Crab

综述了近10a国内外有关重金属、氨态氮、亚硝酸盐、有机污染物等几种环境因子对虾蟹类非特异性免疫影响的研究成果,以期为通过改善养殖环境条件提高虾蟹类自身免疫抗病力提供理论依据。同时,为进一步深入开展该领域的研究工作积累资料。This paper attempted to review and evaluate existing information about the effects of heavy metal,ammonia-N,nitrite and the organic pollutants on the non-special immune response of the shrimp and crab,which providing theory for improving the self-immunity of shrimp and crab by meliorating cultural environment. In addition,it could provide information for further study on this field

Expression profile of voltage-gated potassium channel Kv1.3 in aorta of atherosclerosis rats

目的探讨动脉粥样硬化(AS)大鼠主动脉病变局部离子通道kV1.3的表达水平及作用。方法雄性WISTAr大鼠16只,随机分为两组:正常组(8只,予普通饮食+0.9%氯化钠溶液)和AS组(8只,予高脂饮食+维生素d3负荷)。采用组织病理学检查,观察主动脉粥样硬化病变。采用实时定量反转录-聚合酶链反应(rT-PCr)和WESTErn印迹法检测主动脉病变局部kV1.3和白细胞介素(Il)-2、干扰素(Ifn)-γ的表达水平。结果组织病理学检查证实纤维增生性AS斑块。AS组的kV1.3MrnA为(31.48±8.64)x10-3,显著高于正常组的(3.28±0.79)x10-3(P=0.012)。AS组的kV1.3、Il-2、Ifn-γ蛋白表达量分别为0.691±0.067、0.611±0.055、0.759±0.050,均显著高于正常组的0.490±0.052、0.299±0.058、0.273±0.052(P值均<0.01)。结论 kV1.3在主动脉粥样硬化病变局部的表达增高。kV1.3可能在AS的发生和发展过程中发挥着重要的作用。Objective To investigate the expression of voltage-gated potassium channel Kv1.3 in the aorta of a rat model of atherosclerosis and its role in the progress of atherosclerotic plaque formation.Methods A total of 16 male Wistar rats were randomly divided into normal control(normal diet and saline)and atherosclerosis group(high lipid diet+Vitamin D3 overload),with 8 rats in each group.In 16 weeks later,all rats were killed after weighing,and their blood samples and aorta were collected.Pathological changes of the rat aortic artery were observed with HE staining.Real time RT-PCR and Western blotting analysis were used to determine the mRNA and protein expression of Kv1.3 and the protein expressions of interleukin(IL)-2 and interfereron(IFN)-γ.Results Pathological changes showed that fiber proliferative atherosclerotic plaques were found in the aorta of atherosclerosis group,with inflammatory cells infiltrating in the local lesion.Real time RT-PCR analysis showed that the expression of Kv1.3 mRNA in the aorta was increased significantly in the atherosclerosis rats than that in the controls([31.48±8.64]×10-3 vs.[3.28±0.79]×10-3,P<0.05).Western blotting analysis showed that the protein expression of Kv1.3,IL-2 and IFN-γ in the aorta were also increased significantly in the atherosclerotic rats than that in the controls(Kv1.3[0.691±0.067] vs.[0.490±0.052],IL-2 [0.611±0.055] vs.[0.299±0.058],IFN-γ [0.759±0.050] vs.[0.273±0.052],P<0.01 n=8).Conclusion The expression of Kv1.3 potassium channels is increased in the plaques of atherosclerotic rats.Kv1.3 may play an important role in the development and progression of atherosclerosis.国家自然科学基金资助项目(30871045

Retrieving the Most Prevalent Small Fullerene C(56)

通讯作者地址: Xie, SY (通讯作者),Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] of China,20721001 ,21031004 973 Program 2007CB815301 2011CB93590

Effect of raindrop impact on nutrient losses under different near-surface soil hydraulic conditions on black soil slope

目前,土壤水分饱和和壤中流条件下,雨滴打击对养分流失的影响尚不清楚。通过3个近地表土壤水文条件(自由入渗、土壤水分饱和与壤中流)下,土槽上方架设与不架设尼龙纱网模拟降雨对比试验,研究雨滴打击对黑土坡面侵蚀过程及NO3-N、NH4-N与PO4-P随径流和侵蚀泥沙迁移的影响。结果表明,纱网覆盖消除雨滴打击后坡面侵蚀量和泥沙浓度分别减少59.4%&mdash;71.6%和57.3%&mdash;73.0%,不同水文条件下减少量的排序为:自由入渗&gt;壤中流&gt;土壤水分饱和。消除雨滴打击后养分随径流流失的减少仅在自由入渗条件下体现较明显,该水文条件下NO3-N、NH4-N与PO4-P流失分别减少33.3%、23.1%和40.7%;3种水文条件下,消除雨滴打击均明显减少养分随泥沙的流失,其中自由入渗条件下减少效果最明显,该水文条件下,NO3-N和NH4-N流失分别减少20.9%&mdash;54.9%和25.0%&mdash;62.3%,而PO4-P流失减少在74.6%以上。雨滴打击增大了NO3-N的淋失,但对NH4-N与PO4-P的淋失几乎无影响。消除雨滴打击后,自由入渗条件下养分的等效径流迁移深度减少26.7%&mdash;42.6%,而土壤水分饱和与壤中流条件下基本无变化。以上..

Soil Aggregate Transport During Soil Erosion Process Under Different Soil Surface Conditions on Black Soil Slope Farmland

土壤侵蚀过程中,土壤团聚体的迁移反映了团聚体的破碎程度及径流的搬运能力,直接影响着侵蚀强度。以东北典型薄层黑土区耕层土壤为研究对象,采用模拟降雨试验方法,研究裸露休闲、纱网覆盖与秸秆覆盖3种地表条件下不同粒级(&gt;5mm,2～5mm,1～2mm,0.5～1mm,0.25～0.5mm和&lt;0.25mm)土壤团聚体的迁移特征。结果表明:(1)纱网覆盖与秸秆覆盖具有削减径流和抑制侵蚀的作用,其中抑制产沙作用更明显,减沙率分别为75%和99%以上;(2)3种地表条件下,&lt;0.25mm团聚体是主要的流失粒级,其占到团聚体流失总量的50%以上;各粒级流失量均表现为:裸露休闲&gt;纱网覆盖&gt;秸秆覆盖;(3)纱网覆盖和秸秆覆盖下的团聚体粒级分布与裸露休闲的相比,差异较为明显的均是&lt;0.25mm粒级,该粒级流失量较裸露休闲的分别减少74.62%和99%;秸秆覆盖与纱网覆盖之间差异明显的是&lt;0.25mm与1～5mm粒级,流失量较纱网覆盖的分别减少97.81%和86.03%;(4)秸秆覆盖下团聚体平均重量直径表现为最大;分形维数和平均重量比表面积均表现为:裸露休闲&gt;纱网覆盖&gt;秸秆覆盖

降雨侵蚀过程中黑土团聚体流失特征

坡面侵蚀过程中土壤团聚体流失可直接反映土壤团聚体的破碎程度以及雨滴打击和径流搬运之间的相互作用。基于模 拟降雨试验，研究降雨侵蚀过程中黑土坡面土壤团聚体流失特征。试验处理包括黑土区常见的2 个侵蚀性降雨( 50、100mm/h 降雨强度) 和2 个坡度( 5&deg;和7．5&deg;) 以及2 种地面处理( 裸露休闲和秸秆覆盖) 。结果表明: 裸露休闲处理下径流含沙量是秸秆 覆盖处理的27．5&mdash;141．3 倍，且不同处理下含沙量最大值均出现在降雨初期。覆盖秸秆与无覆盖试验处理下土壤团聚体流失均 以＜0．25 mm 微团聚体为主。秸秆覆盖试验处理下泥沙中＜0．25 mm 微团聚体流失量占团聚体流失总量的34．5%&mdash;56．8%，而在 裸露休闲处理下其值达到82%以上。秸秆覆盖处理下的各粒级团聚体流失量较裸露休闲试验处理减少了33．3%以上，其中差 异最明显的是&ge;1 mm 粒级团聚体与＜0．25 mm 微团聚体，二者分别较裸露休闲处理减少了43．1%&mdash;96．4%和99．0%以上。秸秆 覆盖处理下的0．25&mdash;2 mm 粒级团聚体流失比例较裸露休闲处理明显增加。秸秆覆盖试验处理下流失团聚体的平均重量直径 ( MWD) 和几何平均直径( GMD) 分别是裸露休闲处理的1．5&mdash;2．9 和1．7&mdash;2．0 倍; 而秸秆覆盖处理下的平均重量比表面积 ( MWSSA) 和分形维数( D) 较裸露休闲处理分别减少了26．2%&mdash;32．9%和5．1%&mdash;6．7%。</p

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坡面侵蚀过程中土壤团聚体迁移反映了团聚体的破碎程度以及雨滴打击和径流搬运之间的相互作用。基于模拟降雨试验,研究了黑土坡面不同粒级土壤团聚体的迁移特征。研究结果表明,同干筛处理相比,湿筛后&ge;0.25mm粒径的水稳性团聚体含量为52%,其较干筛处理减少24%。湿筛后土壤团聚体的粒级分布以&lt;0.25mm团聚体居多;湿筛处理后&gt;1mm粒级的团聚体含量较干筛处理减少了83.8%。在50和100mm/h两个降雨强度下,团聚体流失以&lt;0.25mm的微团聚体为主,其流失量占团聚体流失总量的80%以上,且不同降雨强度下微团聚体流失量与含沙浓度存在显著正相关关系。50mm/h降雨强度下微团聚体流失量随降雨历时的增加呈先快速增加后递减,最后趋于相对稳定的变化趋势;而100mm/h降雨强度下,其变化趋势则表现为先快速增加后缓慢上升趋势。&ge;0.25mm各粒级团聚体的流失比例和流失团聚体的平均重量直径(MWD)均随降雨强度的增加而减小,反映了大雨强下雨滴打击对团聚体的分散作用。Ａｂｓｔｒａｃｔ：Ｔｈｅ　ａｇｇｒｅｇａｔｅ　ｍｏｖｅｍｅｎｔ　ｄｕｒｉｎｇ　ｈｉｌｌｓｌｏｐｅ　ｅｒｏｓｉｏｎ　ｐｒｏｃｅｓｓ　ｒｅｆｌｅｃｔｓ　ｔｈｅ　ｉｎｔｅｒａｃｔｉｏｎ　ａｍｏｎｇ　ｔｈｅ　ａｇｇｒｅｇａｔｅｂｒｅａｋａｇｅ，ｒａｉｎｄｒｏｐ　ｉｍｐａｃｔ　ａｎｄ　ｒｕｎｏｆｆ　ｔｒａｎｓｐｏｒｔａｔｉｏｎ．Ｔｈｅ　ｓｉｍｕｌａｔｅｄ　ｒａｉｎｆａｌｌ　ｅｘｐｅｒｉｍｅｎｔ　ｗａｓ　ｄｅｓｉｇｎｅｄ　ｔｏ　ｑｕａｎｔｉｆｙｍｏｖｅｍｅｎｔ　ｃｈａｒａｃｔｅｒｉｓｔｉｃｓ　ｏｆ　ｖａｒｉｏｕｓｌｙ　ｓｉｚｅｄ　ａｇｇｒｅｇａｔｅｓ　ｏｎ　ｈｉｌｌｓｌｏｐｅ　ｆａｒｍｌａｎｄｓ　ｏｆ　ｔｈｅ　ｂｌａｃｋ　ｓｏｉｌ　ｒｅｇｉｏｎ，ＮｏｒｔｈｅａｓｔＣｈｉｎａ．Ｔｈｅ　ｒｅｓｕｌｔｓ　ｓｈｏｗｅｄ　ｔｈａｔ　ｔｈｅ　ｃｏｎｔｅｎｔ　ｏｆ＞０．２５ｍｍ　ｗａｔｅｒ－ｓｔａｂｌｅ　ｓｏｉｌ　ａｇｇｒｅｇａｔｅ　ｗａｓ　ａｂｏｕｔ　５２％，２４％ｌｏｗｅｒ　ｔｈａｎ　ｔｈｅ　ｃｏｎｔｅｎｔｓ　ｏｂｔａｉｎｅｄ　ｔｈｒｏｕｇｈ　ｄｒｙ　ｓｉｅｖｉｎｇ，ｗｈｉｌｅ＞１ｍｍ　ｓｏｉｌ　ａｇｇｒｅｇａｔｅｓ　ｃｏｎｔｅｎｔｓ　ｗａｓ　８３．８％ｌｏｗｅｒ．Ｔｈｅ　ｍａｊｏｒｉｔｙ　ｏｆ　ｔｈｅ　ｗａｔｅｒ－ｓｔａｂｌｅ　ｓｏｉｌ　ａｇｇｒｅｇａｔｅｓ　ｗａｓ＜０．２５ｍｍ　ａｇｇｒｅｇａｔｅｓ．Ｕｎｄｅｒ　ｒａｉｎｆａｌｌｓ　ｏｆ　５０ａｎｄ１００ｍｍ／ｈ　ｉｎｔｅｎｓｉｔｉｅｓ，＜０．２５ｍｍ　ａｇｇｒｅｇａｔｅｓ　ｃｏｎｔｒｉｂｕｔｅｄ　ｍｏｓｔ　ｏｆ　ｔｈｅ　ａｇｇｒｅｇａｔｅ　ｌｏｓｓ，ａｃｃｏｕｎｔｉｎｇ　ｆｏｒ　ｍｏｒｅｔｈａｎ　８０％ ｏｆ　ｔｈｅ　ｔｏｔａｌ　ａｇｇｒｅｇａｔｅ　ｌｏｓｓ．Ｔｈｅ　ｍｉｃｒｏ－ａｇｇｒｅｇａｔｅ　ｌｏｓｓ　ｒａｔｅｓ　ｗｅｒｅ　ｆｏｕｎｄ　ｓｉｇｎｉｆｉｃａｎｔｌｙ　ｐｏｓｉｔｉｖｅｌｙｃｏｒｒｅｌａｔｅｄ　ｗｉｔｈ　ｓｅｄｉｍｅｎｔ　ｃｏｎｃｅｎｔｒａｔｉｏｎｓ　ｕｎｄｅｒ　ｂｏｔｈ　ｒａｉｎｆａｌｌ　ｉｎｔｅｎｓｉｔｉｅｓ．Ａｓ　ｔｈｅ　ｒａｉｎｆａｌｌ　ｔｉｍｅ　ｅｘｔｅｎｄｅｄ，ｔｈｅｌｏｓｓ　ｒａｔｅ　ｏｆ　ｍｉｃｒｏ－ａｇｇｒｅｇａｔｅ　ｒａｐｉｄｌｙ　ｉｎｃｒｅａｓｅｄ　ｆｒｏｍ　ｔｈｅ　ｉｎｉｔｉａｌ　ｓｔａｇｅ　ａｎｄ　ｔｈｅｎ　ｄｅｃｒｅａｓｅｄ　ｉｎ　ａ　ｓｈｏｒｔ　ｐｅｒｉｏｄ　ｏｆｔｉｍｅ　ａｎｄ　ｒｅａｃｈｅｄ　ａ　ｒｅｌａｔｉｖｅｌｙ　ｓｔａｂｌｅ　ｓｔａｇｅ　ｕｎｄｅｒ　５０ｍｍ／ｈ　ｒａｉｎｆａｌｌ　ｉｎｔｅｎｓｉｔｙ；ｗｈｉｌｅ　ｕｎｄｅｒ　１００ｍｍ／ｈ　ｒａｉｎｆａｌｌｉｎｔｅｎｓｉｔｙ，ｔｈｅ　ｍｉｃｒｏ－ａｇｇｒｅｇａｔｅ　ｌｏｓｓ　ｒａｔｅ　ｉｎｃｒｅａｓｅｄ　ｑｕｉｃｋｌｙ　ａｎｄ　ｉｎｃｒｅａｓｅｄ　ｓｌｏｗｌｙ　ａｆｔｅｒ　ｔｈｅ　ｐｅａｋ　ｖａｌｕｅ　ｗａｓｒｅａｃｈｅｄ．Ｔｈｅ　ｐｒｏｐｏｒｔｉｏｎ　ｏｆ＞０．２５ｍｍ　ａｇｇｒｅｇａｔｅｓ　ｌｏｓｓ　ａｎｄ　ＭＷＤ　ｏｆ　ａｇｇｒｅｇａｔｅｓ　ｌｏｓｓｅｓ　ｄｅｃｒｅａｓｅｄ　ｗｉｔｈ　ｔｈｅｉｎｃｒｅａｓｅ　ｏｆ　ｒａｉｎｆａｌｌ　ｉｎｔｅｎｓｉｔｙ，ｉｎｄｉｃａｔｉｎｇ　ｔｈａｔ　ｒａｉｎｄｒｏｐ　ｉｍｐａｃｔ　ｈａｄ　ｐｌａｙｅｄ　ａ　ｇｒｅａｔ　ｒｏｌｅ　ｉｎ　ｓｅｐａｒａｔｉｎｇ　ａｇｇｒｅｇａｔｅｓ