我国厦门地区散发性戊型肝炎病毒部分核苷酸序列分析

自新疆发生戊型肝炎（HE）暴发流行以来，已陆续报道我国其他地区存在散发感染〔1〕。本实验室已报道我国广州地区的散发性HEV与我国北方和国外HEV在基因结构上有明显不同〔2，3〕。最近又对从厦门地区急性HE病人的血清中检出的HEVrnA作序列分析，结果...总后指令性课

Study On Anti HEV

戊型肝炎病毒（HEV）和戊型肝炎已被研究多年，许多作者从流行病学、血清学以及病毒基因方面，作了许多工作［1~3］，在诊断试剂方面，也有不少报告［4~6］.我们在研究中发现，HEV抗体有不同亚型，现将初步结果报告如下.1材料和方法1.1材料HEV抗原的...The anti HEV in serum of the normals and the patients with hepatitis was detected by using three strains HEV antigen:HEV 87A, HEV G93, HEV F86.The results showed that the anti HEV.IgM was not detected on the normals, and the positive of anti HEV.IgM to HEV 87A, HEV G93.HEV F86 have 7,12,23 cases on the patients with hepatitis, respectively.The positive of anti HEV.IgG to HEV 87A, G93, F86 have 8,8,5 cases on the normals; 24, 30 and 19 cases on the patients with hepatitis, respectively.On the patients with hepatitis, only one positive of anti HEV.IgM to 87A, G93, F86 but negative to the other two antigen have 0, 4, 12 cases, respectivelg; only one posictive of anti HEV.IgG to 87A, G93 or F86, but the negative to the other two antigen have 7,8,3 cases, respectively.Two positive of anti HEV.IgM to 87AG93 or 87AF86 or G93F86, but the negative to the other one antigen have 0,3,5 cases, two positive of anti HEV.IgG to them have 10,2,6 cases, respectively.On the normals, all positive of anti HEV.IgG to the three antigen have 3 cases,it has 8 cases on the patients with hepatitis.On these patients, all positive of IgM to the three antigen have 3 cases.It was point out that there are different subgroups in anti HEV, so except to study different subgroup of HEV gene, we must take notice of existing the subgroup of anti HEV in diagnostic reagent of HEV

The Clinical Application of HEV-87A Antigen EIA KIT

我国戊型肝炎全病毒抗原抗HEV·IgM酶联诊断盒的临床应用廖绵初，干侣仙，黄如统，李晓萸，李德荣（厦门大学抗癌研究中心）（军事医学科学院五所）赵炳华（浙江医科大学第一附属医院）从1989年正式确立戊型肝炎HEPATITISE以来，许多国家都研制其诊断...hepatitis patient's sera were detected by HEV-87A antigen (inactivated cultue virus)EIA KIT.82 of them were anti-HEV.IgM positive by Diagnostic Biotechnology (Pte)Ltd (DBL)EIA kit; 90 of them were anti--HEV IgM positive and 88 were anti-HEV IgG positive by HEV-87A EIA kit.5 negative sera detected by DBL kit were neutralized by HEV87A antigen.4 of 5 could be neutralized, 1 of the 5 sera could not be neutralized.The results show that our EIA kit is more sensitive and speciFic.Among early patients with hepatitis E, the determination of anti-HEV IgM is better than that of anti-HEV IgG and it was discovered that patients with hepatitis E are co-inFectous with hepatitis A,B,C,D virus some times

Gene analysis of acute sporadic HEV in the southern areas of China

目的进一步证实我国南方地区存在戊型肝炎病毒（HEV）不同的基因序列。方法用逆转录套式聚合酶链反应（rT-nESTEdPCr）检测8例厦门地区急性散发性成型肝炎患者血清中的HEVrnA，其中2例阳性；测定了广州分离株g93—2株病毒第5代培养液的HEVrnA。对阳性PCr产物进行克隆、核苷酸序列分析。结果厦门株X-S1与广州g93-2株病毒核苷酸和氨基酸序列的同源性为992%和975%；厦门株（X-S1）和广州株（g93-2）与缅甸株（bur—121）和我国新疆株（87A）的核苷酸和氨基酸序列的同源性为79.9%和86.3%，与墨西哥株的同源性为774%和%.3%。结论经序列分析结果进一步证实我国南方地区确实存在一种不同的HEV基因型。ON.ctiv.To investigate the existing different bine wnuences of tiepatitis E virus (HEV)in the southern areas of China.Methods Eight acute--phase sera of sporadic patients with hepatitis E inXiamen were tested by reverse transcription-- polymerase chain reaction(RT -- P C R ).Two cases of them werepositive.The fad pessagu culture of Guamphou G93--2 Strain of HEV was also detected by RT--PCR.Thepositive RT--PCR products underwent cloning and nucleotide wnuencing.R.sults The homology of nucleotide and the amino acid between Xiamen X-S1 and Guangzhou G93--2 strain are 99.2% and 97.5%.Thehomology of nucleotide and the amino acid wnuences of Xiamen Strain and GuangZhou strain are 79.9%and 86.3% compared with the Burmese Strain (Bur--121) and our country Xitoiang Strain (87A), and are77.4% and 86.3% against Median Strain.Conclu.ion The results suggest that there is a different grnotypeof HEV in the southern areas of China.总后卫生部基金!960803