Mitochodrial DNA COI Sequence Analysis of Calanus sinicus (Copepod) in Qingdao Waters

采用苯酚 氯仿 异戊醇(25∶24∶1)提取、异丙醇沉淀、酒精洗涤、TE缓冲液保存方法提取了采自胶州湾青岛海域的中华哲水蚤基因组DNA;以相应引物经PCR扩增得到线粒体DNA细胞色素氧化酶 I亚基基因(mtCOI)片段;PCR产物采用化学法进行质粒重组(载体pGEM T Easy Vector)、热休克转化重组质粒至大肠杆菌感受态细胞(JM109)、氨苄LB培养基扩大培养;测序.结果表明,中华哲水蚤线粒体COI碱基大小为710 bp(国际基因库索引号AY665663),其碱基组成A、C、G、T含量分别为24.23%、19.15%、22.54%和34.08%;G+C含量为41.69%,A+T为58.31%. 【英文摘要】 Calanus sinicus is a dominant species in the zooplankton communities of the Yellow Sea,Bohai Sea and the East China Sea, and is important food for many pelagic fishes especially their larvae.The specimens in the present study were collected from Qingdao Waters in May of 2002,by horizontal haul.The genomic DNA of Calanus sinicus was extracted by phenol:Chloroform:isoamyl alcohol (25241) and receipted by isopropyl alcohol.Mitochondrial DNA COI gene fragments were amplified successfully under our PCR conditi...国家重点基础研究发展规划项目 ( 973 项目)(G19990437-08

Mitochodrial DNA COI Sequence Analysis of Calanus sinicus (Copepod) in Qingdao Waters

采用苯酚 氯仿 异戊醇(25∶24∶1)提取、异丙醇沉淀、酒精洗涤、TE缓冲液保存方法提取了采自胶州湾青岛海域的中华哲水蚤基因组DNA;以相应引物经PCR扩增得到线粒体DNA细胞色素氧化酶 I亚基基因(mtCOI)片段;PCR产物采用化学法进行质粒重组(载体pGEM T Easy Vector)、热休克转化重组质粒至大肠杆菌感受态细胞(JM109)、氨苄LB培养基扩大培养;测序.结果表明,中华哲水蚤线粒体COI碱基大小为710 bp(国际基因库索引号AY665663),其碱基组成A、C、G、T含量分别为24.23%、19.15%、22.54%和34.08%;G+C含量为41.69%,A+T为58.31%.Calanus sinicus is a dominant species in the zooplankton communities of the Yellow Sea,Bohai Sea and the East China Sea, and is important food for many pelagic fishes especially their larvae.The specimens in the present study were collected from Qingdao Waters in May of 2002,by horizontal haul.The genomic DNA of Calanus sinicus was extracted by phenol:Chloroform:isoamyl alcohol (25241) and receipted by isopropyl alcohol.Mitochondrial DNA COI gene fragments were amplified successfully under our PCR conditions,then were recombined with pGEM-T Easy Vector (Promega Co.) and sequenced.The length of the sequence (accession no.AY665663 in GenBank) was 710 base pairs,and its composition (A,C,G and T) was 24.23%、19.15%、(22.54%)和34.08%, respectively.Alignment was completed between the mtCOI sequence collected from Qiaodao Waters and from Inland Sea,Japan (accession no.AF332769,GenBank),and the identity by calculated with DNAMAN software is 99.53%.The correctness and significance of nucleotide sequence met in our study was discussed in the present paper.国家重点基础研究发展规划项目 ( 973 项目)(G19990437-08

Analysis of Mitochondrial DNA CO I Gene Fragment of Pseudeuphausia sinica

【中文摘要】 采用苯酚-氯仿提取、异丙醇沉淀提取中华假磷虾基因组DNA;以相应引物经PCR扩增得到线粒体DNACOI片段;PCR产物采用化学法与载体(pGEM-TEasyVector)重组基因、热休克法转化重组质粒至感受态大肠杆菌(JM109)、氨苄LB培养基扩大培养;测序.结果表明,中华假磷虾线粒体COI碱基709bp,其碱基组成A、T、G、C含量分别为28 59%、35 35%、17 61%和18 45%(国际基因库索引号AY754819);与同科内其它3属10种磷虾的mtCOI基因片段核苷酸组成相似. 【英文摘要】 Pseudeuphausia sinica (Euphausiacea, Crustacea) is abundant in the coastal and neritic waters of the East China Sea and the Southern Yellow Sea all the year round, and is the important food for many fishes, especially for fish larvae. The genomic DNA of Pseudeupharsia sinica, collected from Xiamen waters in May of 2002, was obtained by phenol: Chloroform:isoamyl alcohol (25:24:1) extraction and isopropyl alcohol precipitation. Mitochondrial DNA COI gene was successfully amplified and sequenced. 709 base pai...国家重点基础研究发展规划项目(973)(G1990437-08)资

Analysis of Mitochondrial DNA CO I Gene Fragment of Pseudeuphausia sinica

采用苯酚-氯仿提取、异丙醇沉淀提取中华假磷虾基因组DNA;以相应引物经PCR扩增得到线粒体DNACOI片段;PCR产物采用化学法与载体(pGEM-TEasyVector)重组基因、热休克法转化重组质粒至感受态大肠杆菌(JM109)、氨苄LB培养基扩大培养;测序.结果表明,中华假磷虾线粒体COI碱基709bp,其碱基组成A、T、G、C含量分别为28 59%、35 35%、17 61%和18 45%(国际基因库索引号AY754819);与同科内其它3属10种磷虾的mtCOI基因片段核苷酸组成相似. 【英文摘要】 Pseudeuphausia sinica (Euphausiacea, Crustacea) is abundant in the coastal and neritic waters of the East China Sea and the Southern Yellow Sea all the year round, and is the important food for many fishes, especially for fish larvae. The genomic DNA of Pseudeupharsia sinica, collected from Xiamen waters in May of 2002, was obtained by phenol: Chloroform:isoamyl alcohol (25:24:1) extraction and isopropyl alcohol precipitation. Mitochondrial DNA COI gene was successfully amplified and sequenced. 709 base pai...国家重点基础研究发展规划项目(973)(G1990437-08)资

四株草鱼呼肠孤病毒毒株的细胞感染特性比较研究

本文首次对低温保存的三株草鱼呼肠孤病毒GCRV873 、GCRV875、GCRV876与新分离的GCRV991毒株进行了细胞培养与病毒感染特性等比较研究。结果表明 ,GCRV873 、GCRV875、GCRV876在 - 30℃保存 10年后仍然具有一定的感染性 ,其滴度均在 10 2 TCID50 /mL以上 ,略低于从病鱼组织分离的GCRV991毒株的滴价。经传代培养后 ,四株GCRV的毒力逐渐升高 ,并趋于稳定 ;当感染复数 (MOI)为 0 .0 5PFU/cell时 ,测定四株GCRV的滴度均

High-density matter: current status and future challenges

There are many fascinating processes in the Universe which we observe in more and more in detail thanks to increasingly sophisticated technology. One of the most interesting phenomena is the life cycle of stars, their birth, evolution and death. If the stars are massive enough, they end their lives in the core-collapse supernova explosion, the one of the most violent events in the Universe. As the result, the densest objects in the Universe, neutron stars and/or black holes are created. Naturally, the physical basis of these events should be understood in line with observation. The current status of our knowledge of processes in the life of stars is far from adequate for their true understanding. We show that although many models have been constructed their detailed ability to describe observations is limited or non-existent. Furthermore the general failure of all models means that we cannot tell which are heading in the right direction. A possible way forward in modeling of high-density matter is outlined, exemplified by the quark-meson-coupling model (QMC). This model has a natural explanation for the saturation of nuclear forces and depends on very few adjustable parameters, strongly constrained by the underlying physics. Latest QMC results for compact objects and finite nuclei are presented

草鱼呼肠孤病毒新分离株(GCRV_(991))的病毒学特性分析

从湖南长沙分离到一株致病性强的草鱼呼肠孤病毒 (GCRV991) ,该病毒能使草鱼CIK ,肥头鲤FHM细胞产生明显的CPE ,对水生动物BF2 ,EPC及哺乳动物BHK ,VERO细胞株不敏感。中和实验显示 ,GCRV873 抗体能有效地中和GCRV991病毒颗粒 ,形成抗原抗体免疫复合物。纯化的病毒核酸与蛋白经SDS PAGE分离 ,分别呈现 11条清晰的核酸带及 5条主要与 2条微量结构多肽图谱 ,其核酸蛋白分子量大小与GCRV873 相近似。该毒株基因组总分子量为 14.48× 10 6kD ,

Identification and Characterization of Vibrio pomeroyi Isolated from Apostichopus japonicus with Skin Ulcer Syndrome During Aestivation

以患有腐皮综合征的度夏仿刺参(APOSTICHOPuS JAPOnICuS)为对象,从其体壁病灶处分离纯化获得一株病原细菌.通过传统细菌鉴定方法对病原菌的形态学、生理生化特征和培养特征进行研究;以16SrrnA基因测序为基础,结合基质辅助激光解析电离飞行时间质谱技术(MAldI-TOf MS)进行鉴定,结果表明该菌株在分类上属于伯麦罗氏弧菌(VIbrIO POMErOyI);人工感染实验验证了不论是注射感染还是浸浴感染,该菌株均能导致仿刺参发病甚至死亡.此外,将本次实验获得的伯麦罗氏弧菌菌株SuS(V.POMErOyI SuS)与伯麦罗氏弧菌的其他菌株,如V.POMErOyI lMg20537T(典型菌株)、V.POMErOyI 929进行比较,探讨了来自不同海域的菌株生理生化特征与环境因子之间的关系.研究结果显示:伯麦罗氏弧菌为南移度夏仿刺参腐皮综合征的病原菌,而且表明MAldI-TOf MS技术结合微生物数据库MAldI bIOTyPEr系统的鉴定方法准确、便捷、重复性高,非常适合于传统鉴定方法易混淆的种类鉴别,这为南移仿刺参的疾病检查和预防奠定了基础.The sea cucumber,Apostichopus japonicus(Selenka,1867),is a major aquaculture species along the coastline of northern China.Because of increasing demand of the profitable species,culture of A.japonicus has expanded towards to the southern region of China including Fujian,Guangdong Provinces.However,as a temperate species,A.japonicusis more easily infected by environmental bacteria during aestivation,and skin ulcer syndrome is proved to be the major disease of sea cucumber.In this study,a dominant bacterium strain was isolated from the body wall of diseased cultured sea cucumber(A.japonicus)infected with skin ulcer syndrome.Phenotypic characterization of the bacteria was investigated with traditional biochemical tests.Genotypic characterization was evaluated by 16SrRNA gene sequencing technique,along with matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-Tof-MS).The phenotypic and genotypic characterization proved that the pathogen was Vibrio pomeroyi.Meanwhile,the artificial infection test proved that V.pomeroyi could cause severe infections and even death whether injection or immersion.Additionally,comparing V.pomeroyi SUS isolated from China with other strains of V.pomeroyi,including LMG 20537T(type strain)isolated from Brazil and V.pomeroyi 929isolated from Peter the Great Bay showed distinctly different tolerance pattern with environmental factors,e.g.salinity and temperature.For the first time this paper reported the isolation of the pathogen of A.japonicus with skin ulcer syndrome during aestivation in southern China and MALDI-Tof MS analysis was proved to be an accurate and rapid method for identification and differentiation of common pathogens.福建省海洋与渔业厅科研专项(闽海渔科(2012)003号

开都河流域下游绿洲土壤盐渍化特征及其光谱分析[J]

以新疆开都河流域下游绿洲为研究区,通过土壤样品化学分析及其光谱反射率的测量,分析了土壤盐渍化特征以及土壤反射光谱曲线与土壤盐渍程度之间的关系;基于统计分析的方法,建立了表征土壤盐渍化特征的因子与其高光谱数据的多元回归模型。研究结果表明:研究区土壤pH值的平均值大于8.0,属于弱碱性土壤;土壤盐分含量呈T型分布;盐渍化土壤的光谱反射率曲线特征在形态上相似,变化平缓;在可见光-近红外波段,盐渍化土壤的光谱反射率曲线形状基本上由五个折线段和四个吸收带组成

基于GIS土壤侵蚀监测研究——以新疆为例[J]

以新疆为例,应用GIS技术对水利部TM影遥影像进行栅格处理,得到研究区域中水力、风力及冻融侵蚀数据,对其进行归一化处理,得到侵蚀的不同程度等级例表.然后分别对以上三种侵蚀采用主导因素法,计算出三种侵蚀在研究区域的面积及与总侵蚀面积的百分比,最后对区域内侵蚀分布情况进行地域分析.研究表明,风力侵蚀在新疆最为严重,同时还存在着两种侵蚀共同作用的区域