Study on Fluorimetry for Determination of Phenylalanine Using Enzyme Substituted by the UV-Light

在碱性介质中， 光子可以取代苯丙氨酸氧化酶使苯丙氨酸分解并生成H2 O2 ， 然后用氯化血红素作为模拟酶检测H2O2 ， 从而建立间接测定苯丙氨酸的新方法.In alkaline medium,the photon can substitute phenylalanine oxydase,and cause phenylalanine to decompose and produce H 2 O 2 ,which are detected by using HPA to be coupled and produce strong fluorescent substance in present of mimetic peroxydase-hemin.Thus,a new fluorimetry for the determination of phenylalanine was developed.福建省自然科学基金!（B96003

Rh/SiO_2和Rh/NaY催化剂上合成气反应的高压原位红外光谱研究

采用高压原位FT -IR技术 ,对比研究了CO加H2 反应条件下Rh/SiO2 和Rh/NaY催化剂表面反应中间物种 .在Rh/SiO2 表面上 ,无论在常压还是在 1.0MPa合成气中 ,只观察到线式和桥式吸附CO .而在常压合成气中 ,Rh/NaY上不仅存在上述CO吸附物种 ,而且还有孪生型的Rh(I) (CO) 2 和少量Rh6 (CO) 16 ;当合成气压力升至 1.0MPa后 ,Rh(I) (CO) 2 迅速转化成Rh6 (CO) 16 和在 2 0 42cm-1产生吸收的单核羰基Rh物种 ,与此同时催化剂表面还生成了单齿和双齿乙酸根物种 ;这些在高压下生成的物种在合成气压力重新降回到常压时依然稳定存在 .研究Rh/NaY上合成气反应表面物种与H2 的反应行为表明单齿乙酸根很可能是反应的活性中间物 .这些结果说明Rh/NaY催化剂在高压合成气中的重构是诱发选择生成乙酸反应的基

In-situ FT-IR study of high pressure syngas conversion over Rh/SiO2 and Rh/NaY catalysts

High pressure syngas [V(CO) : V(H-2) = 1] conversion over unpromoted Rh catalyst supported on silica and zeolite NaY were studied at 250 degreesC with an in-situ. IR cell that avoided contamination of iron carbonyls. Change of the syngas pressure produced no effect on the IR spectrum of Rh/SiO2; bridged and linear CO on Rh clusters were the only detectable surface species under 0.1 to 1.0 MPa of flowing syngas. In addition to the bridged and linear CO species, two types of dicarbonyls [Rh(I)(CO)(2)] and a small amount of Rh-6(CO)(16) were formed when Rh/NaY was exposed to 0.1 MPa syngas. Increasing of the syngas pressure to 1. 0 MPa over Rh/NaY resulted in transformation of the dicarbonyls to Rh-6(CO)(16) and probably a mononuclear medium carbonyl featuring an absorption 2042 cm(-1). The detectable reaction products adsorbed on Rh/NaY catalyst under 1.0 MPa were monodentate and bidentate acetates. These surface species were maintained even after releasing the syngas pressure back to 0.1 MPa. Thus, a remarkable difference exists in the effect of syngas pressure on the strtucture of Rh catalysts: reconstruction of Rh catalyst under high pressure of syngas occurs in zeolite NaY but not on silica. Reactivity of the adsorbed surface species toward hydrogen after the catalyst reconstruction suggests that the monodentate acetate groups are responsible for the selective formation of acetic acid from syngas over the Rh/NaY catalyst

可动凝胶体系渗流流变特性及其表征

通过室内流变实验和渗流流变特性实验,研究了可动凝胶体系的流变特征,分析了可动凝胶体系的损耗模量和储能模量的量值变化关系,阐明了可动凝胶体系的粘弹特性及触变性。根据实验和非牛顿流变学理论,建立了多参数粘弹-触变性本构关系,并在实验和应用中得到验证。模型计算结果与实验数据相吻合,本构方程的稳定性较好

Characterization and rheometry of porous flow for weak gel system

通过室内流变实验和渗流流变特性实验,研究了可动凝胶体系的流变特征,分析了可动凝胶体系的损耗模量和储能模量的量值变化关系,阐明了可动凝胶体系的粘弹特性及触变性.根据实验和非牛顿流变学理论,建立了多参数粘弹-触变性本构关系,并在实验和应用中得到验证.模型计算结果与实验数据相吻合,本构方程的稳定性较好

可动凝胶体系非线性渗流数学模型

为进一步研究油田开发渗流中可动凝胶驱的力学行为特征,在实验研究基础上,通过对渗流机理、渗流规律的分析,研究可动凝胶体系非线性渗流力学行为,揭示可动凝胶体系的渗流特征。结合实验,对可动凝胶流动机理和物理化学性质的研究,根据质量传输流体力学、化学动力学的深入研究,系统地建立了可动凝胶体系驱油渗流数学模型。从而,系统的刻画了可动凝胶体系驱油这一复杂渗流流动数学描述难题,完善可动凝胶体系渗流的理论,为今后可动凝胶体系驱油数值模拟研究和现场应用提供了理论基础

In-situ Photochemical Fluorimetry of Tyrosine and Its Determination

在氨性缓冲溶液中，酪氨酸经２７５ｎｍ紫外光照射后，产生荧光二聚体。据此，建立了现场光化学荧光法测定酪氨酸的新方法。线性范围为 ２．０ × １０－７～ ２．０ × １０－５ ｍｏｌ／Ｌ。检测限及精密度分别为 ３．２ × １０－９ｍｏｌ／Ｌ－１和 １．７％。方法简便、快捷，对其它氨基酸有较好的选择性，可用于啤酒及氨基酸注射液的测定。文章对光化学反应机理作了初步探讨。Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4CI buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2. 0 x 10-7 - 2. 0 x 10-5 mol/L tyrosine. The detection limit is 3 .2 x 10-9 mol/L and relative standard deviation is 1 .7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.福建省自然科学基金资助项目

Light as a Substitute for Glutaminic Acid Oxidase and Fluorimentric Determination of Glutaminic acid

在弱碱性介质中，紫外光的照射引起谷氨酸的光化学反应，产生Ｈ２Ｏ２和ＮＨ３，与谷氨酸氧化酶存在下的酶促反应等效。利用氯化血红素（ｈｅｍｉｎ）作为过氧化酶的替代物，催化Ｈ２Ｏ２与对羟基苯乙酸（ＨＰＡ）的偶合荧光反应，建立了间接荧光法测定谷氨酸的方法。Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H2O2 and HN3 in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H2O2 produced by photochemical reaction oxidase p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.福建省自然科学基金资助项