STUDIES ON ECOLOGICAL EFFECTS OF SECONDARY SALINIZATION ON FACILITY CULTURING SOIL AND BIOREMEDIATION

设施栽培农业在我国蔬菜和其他重要的经济作物栽培中发挥了巨大的作用，取得了显著的经济和社会效应，但是由于其大量化肥的施入，特别是氮肥，致使土壤次生盐渍化问题严重突出，土壤中大量的硝态氮给环境和人们的身体健康造成严重威胁。所以开展对于设施栽培农业次生盐渍化研究，对于正确评价土壤环境中次生盐渍化条件下硝态氮迁移转化行为，帮助人们采取合理的整治措施，解决设施栽培土壤次生盐渍化问题具有十分重要的理论和实践意义。本文以设施栽培大棚次生盐渍化土壤为研究对象，开展了硝态氮对土壤微生物生态毒理效应及生物修复研究。主要研究结果如下： 1、随着栽培时间的增加，设施栽培土壤表层土（0~5cm）有机质变化不明显；而土...Facility agriculture has played a huge role in vegetables and other important economic crop cultivation in our country, and won great profit in both economic and social aspect. But large amount of mineral fertilizer used, especially nitrogen, has made soil secondary salinization more serious, and have attracted much attention in recent decades. It is very important to investigate secondary saliniz...学位：理学硕士院系专业：生命科学学院生物学系_动物学学号：2172006115211

Ecotoxicological Effects of Lead and Copper on Seed Germination of Wheat

通过种子萌发试验,研究了不同浓度的Pb2+和Cu2+对小麦种子萌发、幼苗生长以及对小麦α-淀粉酶活性的生态毒理效应。结果表明:Pb2+在低浓度下(5Mg·l-1)对小麦种子萌发过程中的各项指标皆为抑制作用;Pb2+、Cu2+胁迫对小麦α-淀粉酶活性存在低浓度激活和高浓度抑制的效应,且浓度越高,抑制作用越强;Cu2+对小麦种子萌发各项指标的抑制率大于Pb2+,Pb2+、Cu2+对小麦根生长的毒害作用大于对地上部的影响。By seed germination experiments, We studied ecotoxicological effects of Pb2+, Cu2+ on the seed germination, seedling growth and α-amylase activity of wheat.The results showed that the seed germination and seedling growth of wheat could be promoted at low concentrations of Pb2+ (under 0.5 mg·L-1), while they would be inhibited when the concentration of Pb2+ was high; Cu2+ in high concentrations (above 5 mg·L-1) could reduce the germination rate, the seedling growth and vigor markedly; the activity of α-amylase could be enhanced at low concentrations of Pb2+ or Cu2+ but be inhibited at high concentrations, and the injury became severer with the increase of concentration; the inhibition of Cu2+ on seedling growth indices of wheat were larger than that of Pb2+, the stress effects of Pb2+ or Cu2+ was stronger on seed germination than seedling growth.国家高技术研究发展计划(863计划)项目(2007AA10Z441);上海市科委项目(07DZ12055);上海市科委项目(07JC14025

Effects of Cadmium Stress on Seed Germination and Seedling Growth of Dwarf Beans

为比较系统地探讨Cd2+的生物毒性,作者以水培法研究了不同浓度的Cd2+溶液对四季豆种子萌发和幼苗生长发育过程中植物外部生长指标及内部生理生化变化的影响。结果表明:当Cd2+浓度较低时,Cd2+对种子萌发及幼苗生长发育毒害作用较小,一定浓度范围内(0.05~0.5Mg/l)还有促生作用,高浓度Cd2+(5~100Mg/l)能严重抑制四季豆幼苗根及下胚轴的生长;Cd2+胁迫对四季豆幼苗体内过氧化物酶(POd),超氧化物歧化酶(SOd),过氧化氢酶(CAT)活性存在低浓度激活和高浓度抑制的效应,且同一浓度的Cd2+对四季豆幼苗POd、CAT活性的抑制作用表现为根系大于下胚轴,Cd2+浓度>0.5Mg/l时,根系SOd活性大于下胚轴中的活性;Cd2+胁迫下,四季豆下胚轴和根的丙二醛(MdA)含量增加,且根部含量大于下胚轴的量。Cd2+对矮生四季豆种苗生长发育的影响因Cd2+浓度及幼苗部位的不同而异。In order to explore the biological toxicity of Cd2+more systematically, the effects of Cd2+ on the external and internal physiological and biochemical changes in the process of dwarf beans seed germination and seedling growth were studied with hydroponic culture experiments.The results showed that when the concentration of Cd2+ at low level, the effects of Cd2+ on the seed germination and seedling growth were not significant.Seeds germination were activated at a certain extent when Cd2+ concentration ranged from 0.05mg/L to 0.5 mg/L concentrations.The roots and Hypocotyls of seedling exhibited a significant decrease in growth at higher level of Cd2+ concentrations (5～100mg/L).The activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT)could be enhanced at low Cd2+concentrations but inhibited at higher concentration.The activities of POD, CAT were more inhibited in roots than in Hypocotyls at the same Cd2+ concentrations, while that of SOD was higher in roots than in Hypocotyls when the concentration of Cd2+ was more than 0.5mg/L.The malondialdehyde (MDA) contents were continuously increased with Cadmium exposure in both roots and Hypocotyls.The effects of Cd2+on the dwarf beans’growth were varied with the concentration of Cd2+ and the parts of plants.“2007AA10Z441”项目的资助;“07JC14025”项目的资助;“07DZ12055”项目的资