野外斜坡地表入渗性能与渗透系数测量方法与装置

本发明公开了一种野外斜坡地表入渗性能与渗透系数测量方法，它采用了一种野外斜坡地表双环入渗仪，该装置包括一个用于压入野地斜坡地表土中的同心双环结构的斜坡入渗环（3）和一个用于使斜坡入渗环（3）的内环和外环中的降雨量自适应等于饱和无积水入渗量的同样是同心双环结构的降雨筒（2）以及两个用于向降雨筒（2）的双环中供水的马里奥特水箱（1）。本发明的测量方法是通过实现斜坡地表（含水平地表）的饱和润湿无积水入渗及其降雨量的量测，获得土层表面零压力饱和入渗-时间关系，来获得土层表面的降雨入渗性能和饱和渗透系数

Cloning and expression analysis of large yellow croaker (Larimichthys rocea)IL-10gene

白细胞介素10(Interleukin; 10,IL-10)是一种抗炎细胞因子,可以抑制机体免疫反应。本研究经过分析大黄鱼基因组数据库发现了IL-10同源基因,并对其cDNA编码区序列和; 基因组DNA序列进行了克隆分析。大黄鱼IL-10(LycIL-10)基因由5个外显子和4个内含子构成,其序列全长1; 869bp,其开放阅读框(ORF)长555bp,编码184个氨基酸,其N端的22个氨基酸残基为预测的信号肽,成熟肽由162个氨基酸残基组成,包含; 了脊椎动物IL-10标志性保守序列。LycIL-10的氨基酸序列同其他已知物种的IL-10氨基酸序列的一致性为26.49%~77.01%。Rea; l-time; PCR分析发现LycIL-10在检测的组织中为组成型表达,在脾脏和肌肉中转录水平相对较高。三联灭活细菌疫苗和聚肌苷酸胞苷酸(poly(I∶C)); 刺激后,大黄鱼头肾和脾脏中LycIL-10mRNA的转录水平会显著升高,表明LycIL- 10可能参与抑制大黄鱼由细菌和病毒引起的炎症反应。Interleukin-10(IL-10)is a central anti-inflammatory cytokine that; demonstrates immunosuppressive function. In this research,an; interleukin-10(IL-10)homologue has been identified by analyzed the; genomic data of large yellow croaker(Larimichthys; crocea).Subsequently,the open reading frame(ORF)of cDNA and the genomic; DNA sequences have been cloned and analyzed.The genomic DNA of large; yellow croaker IL-10(LycIL-10) consisted of 1 869bp that contains five; exons and four introns,sharing the same organization with mammalian IL-; 10genes.The open reading frame of LycIL-10consisted 555bp that give a; predicted 184amino acid IL-10molecule. It contains a predicted signal; peptide of 22amino acids in the N-terminal and a mature peptide of; 162amino acids,which contained the vertebrate IL-10family signature.The; LycIL-10exhibits a conserved IL-10motif signature and shares; 26.49%-77.01%amino acid sequence identity with other known; IL-10.Real-time PCR analysis showed that LycIL-10 was constitutively; expressed in all tissues tested,especially in spleen and muscle.The; LycIL- 10mRNA transcription level could significantly increase in the; spleen and head kidney of large yellow croaker after trivalent; inactivated vaccines or poly(I∶C)stimulated,indicating that LycIL-10may; be involved in suppressing the inflammatory induced by bacterium or; virus in the large yellow croaker.国家自然科学基金资助项目; 厦门南方海洋研究中心资助项