Polydopamine-Functionalized Graphene Oxide: A Versatile Nanocarrier for Chemotherapy and Photothermal Therapy

光热治疗是一种新型的肿瘤治疗技术，与传统的治疗手段相比，其具有独特的优点，如侵入性低、恢复时间短及并发症发生率小，而将光热治疗与传统的化学药物治疗结合在一个体系里，使热和化疗药物同时作用于肿瘤部位，能够更有效的杀死癌细胞。 石墨烯作为一种二维碳纳米材料，具有超高的比面积和强的近红外光吸收能力，在药物输送与光热治疗方面有着潜在的应用价值。然而，目前的研究中，石墨烯主要是通过π-π堆叠和疏水性相互作用装载疏水性药物，无法实现对那些与之无相互作用的亲水性药物的装载，同时不能为药物提供免于因直接与生理环境接触而失活的保护。多巴胺（DA），一种包含儿茶酚和氨基官能团的物质，能够在温和的聚合条件下自聚合...Photothermal therapy is a new method for cancer therapy, which has special advantages over traditional cancer treatments, such as minimal invasiveness, short recovery times and few complication rates. And the combination of photothermal therapy and chemotherapy in one system is a more effective way to destroy cancer cells than monotherapy since it can deliver both heat and drug simultaneously to t...学位：理学硕士院系专业：材料学院_生物医学工程学号：3142012115007

Magnetic Water Improve the Tolerance of Corn to Cd Ion

本工作在实验室条件下研究磁化水对玉米耐受重金属镉的影响.测定了几种抗氧化酶的活性,与对照组相比,磁化水处理的植株叶片超氧化物歧化酶和过氧化氢酶活性提高,丙二醛含量减少.因此提出,在重金属镉的胁迫下,磁化水可通过提高超氧化物歧化酶和过氧化氢酶活性,消除镉引发的氧化胁迫,保护了细胞膜的完整性(具体体现为植株体内丙二醛含量较低),从而增强植株对重金属镉的耐受性,使植株免受或减缓伤害.对我国受Cd污染地区玉米的种植具有一定的指导意义.In this study,we investigated the effect of magnetic water to the Cd-induced corn's phenotype in the laboratory.In mechanism,the corn flooded by magnetic water display higher SOD and CAT activities and lower concentration of MDA than the control corn.Thus we concluded that Cd can induced oxidant stress in normal conditions,however,flooding of magnetic water increased the SOD and CAT activities,from this result,the concentration of MDA can be decreased in vivo,and the tolerance to the metal pollution can be improved.These conclusions have significant value for culture of corn and other crop in metal polluted area.国家基础科学人才培养基金项目(J0630649

Estimation of genetic parameters of growth traits and genetic gain of F5 generation of scallop Hongmo No. 1

Argopecten irradians concentricus, natively distributed along the Atlantic coast of the United States, has advantages of fast growth, short growth cycle, and high meat yield. It was introduced into China mainland in 1991 and in 2001 its large-scale aquaculture began in the Beibu Gulf area. More than 20 years since the introduction of A. irradians concentricus, as the number of breeding generations increased, the characteristics of the breeding population deteriorated seriously reflected in small individuals, low survival rates, and low meat yields. To solve the problem of germplasm degradation of A. irradians concentricus, this research team hybridized A. irradians concentricus with the scallop Bohai Red in 2016, and successfully obtained hybrid offspring. After four generations of breeding, a new strain of scallop Hongmo No. 1 was obtained. Hongmo No. 1 has the characteristics of a fast growth rate, high survival rate, and high temperature resistance, and has completely adapted to the environment of Beibu Gulf in the south of China, so it has a good prospect of popularization and breeding. Although Hongmo No. 1 has been bred for multiple generations, the genetic parameters of its growth-related traits are unknown and whether it still has breeding potential is unclear. As a result of successive selection in a closed population, genetic parameters varied between different generations of selected strains. Obtaining estimates of genetic parameters and genetic gain of growth-related traits of scallop Hongmo No. 1, which has experienced numerous generations of selection, is of great significance for subsequent breeding. To evaluate the breeding effect of the new scallop strain Hongmo No. 1, this study compared the selected line and a non-selected line in the F4 generation of Hongmo No. 1 and evaluated the genetic parameters and genetic gain of F5 growth-related traits. The results indicated that in the F5 generation of Hongmo No. 1 there was no significant difference in the survival rate between the selected line and the non-selected line (P>0.05), but the growth traits of the selected line were significantly better than those of the non-selected line (P<0.05). The coefficients of variation of quality traits of the two populations of Hongmo No. 1 F5 were 14.60%-28.30%, showing potential for successive selective breeding. The genetic gain of shell length, shell height, shell width, body weight, adductor muscle weight, soft tissue weight, and shell weight in the F5 generation were 7.25%, 7.13%, 4.86%, 25.42%, 31.60%, 27.70%, and 21.29%, respectively. The realized heritability of shell length, shell height, shell width, body weight, adductor muscle weight, soft tissue weight, and shell weight in the F5 generation were 0.37, 0.36, 0.25, 0.36, 0.32, 0.38, and 0.32, respectively, which are moderate levels of heritability. The results of this study showed that the growth traits of the selected line were better than those of the non-selected line, both of which showed a large coefficient of variation for quality traits, moderate mean genetic gain, and medium realized heritability. These results indicate that the genetic improvement of A. irradians concentricus, using first hybridization and then sub-breeding, has achieved good results. These results provide a basis for further breeding of growth traits in scallop Hongmo No. 1

Growth characteristics and production performance analysis of new scallop strain Zihaimo F_4

To assess the production performance of the scallop new strain Zihaimo and provide references for the breeding effect of the new strain,we conducted a research in the same sea area with the same management mode and breeding cycle.By comparing seven traits of scallop farming population including shell length (SL),shell height (SH),shell width (SB),body mass(BW),adductor muscle mass (AW) and shell mass (SW) and survival rate of the new strain Zihaimo F_4 with the Argopecten irradians concentricus,we analyzed their growth characteristics and production performance.The results indicate that the accumulated survival rate of Zihaimo F_4 was 51.32% when it was cultured from October 13~(th) to April 28~(th) of the next year,and the SL was from 1-1.5 mm to 50-60 mm,which was significantly greater than 29.18% of A.irradians concentricus (P<0.01).However,the SL,SH,SB and SW of the two cultivation groups in the same period had extremely significant differences(P<0.01).It is suggested that the growth rate of the new strain Zihaimo F_4 was higher than that of A.irradians concentricus.Compared with A.irradians concentricus,after 6.5 months of cultivation,the SL,SH,SB,BW and AW of the new strain Zihaimo F_4 increased by 19.50%,19.84%,16.23%,56.30% and 20.20%,respectively

云南玉溪绿孔雀遗传多样性分析:与圈养种群的比较

绿孔雀(Pavo muticus)是备受关注的濒危物种。我国南方曾广泛分布的云南亚种(P.m.imperator)目前收缩到中部、南部和西部的局部地区,近年来陆续开展了野外种群调查,但遗传多样性的分析和评估尚未开展。本研究利用从玉溪市新平和峨山交界处采集的野外绿孔雀自然脱落的羽毛,采用11对微卫星标记分析遗传多样性和结构,并与国内主要圈养种群(建群者来自瑞丽地区和其他未确定的地点)进行比较。结果表明:该野生种群的遗传多样性较低,受到近交的影响,遗传多样性进一步丢失的风险较高。遗传结构上,该野生种群和圈养种群的个体被划分为3个亚群,野生种群作为独立的1个亚群,在遗传上有别于圈养种群所含的2个遗传谱系,说明云南省分布的绿孔雀有着显著而复杂的地理分化。当前需要开展系统的谱系地理学研究,以加强族群之间的基因交流为目标,通过生态廊道或个体转移等方式增加遗传交流的机会,扭转遗传多样性随近交的深化而不断丢失的趋势

羽翈中DNA和糖皮质激素共提取技术的研究

羽毛是鸟类研究、监测和管理中常用的非损伤性材料，可从中提取DNA和激素进行遗传和生理分析。然而，作为高度角化的组织，羽翈中的DNA和激素含量极低，提取较为困难。本研究开发一种能够从羽翈中同时提取总DNA和糖皮质激素的方法，命名为DH-CoEx。以丹顶鹤（Grus japonensis）、绿孔雀（Pavo muticus）为代表的大型鸟类廓羽、尾上覆羽和以栗鹀（Emberiza rutila）、小鹀（E.pusilla）为代表的小型鸟类飞羽、绒羽进行测试和评估。结果显示：DH-CoEx能有效从完全角化的羽翈中同时提取出总DNA及糖皮质激素（皮质醇）；从5 mg样本中提取的线粒体DNA（mtDNA）拷贝数达到104量级，可支持长达1 350 bp的片段扩增；所提取的核DNA（nuDNA）质量足以进行长达200 bp的微卫星等位基因分型，成功率为98.11% ~ 99.60%；提取的皮质醇含量达到了传统单一提取激素方法的水平，足以进行相关生理学分析。以上结果说明，DH-CoEx是一种简易、高效和适用性广泛的方法，实现了角化材料总DNA和糖皮质激素的共提取，可以支持mtDNA和微卫星等分子标记的分析和应激激素的分析，极大地提高了羽毛的利用率，具有广泛的应用潜力