基于GPS的景区旅游团时空行为特征研究——鼓浪屿实证案例

本文以鼓浪屿旅游团为研究对象,从量化分析角度揭示其从整体到局部的时空行为特征。首先,借助GPS设备获取旅游团轨迹,结合现场调研和GIS分析旅游团整体时空特征,进一步通过局部特征点提取和分析,探究人气景点受欢迎的原因。研究有助于从实证案例和量化分析角度分析旅游团的时空行为特征,并提出更具针对性的景区管理策略。国家自然科学基金项目（41671141）福建省自然科学基金项目（2015J01226）中央高校基金项目（20720170046）厦门市科技局项目（3502Z20183005

EFFECTS OF SEAWATER ACIDIFICATION AND THERMAL STERSS ON THE ANTIOXIDANT RESPONSES AND ENERGY METABOLISM OF ALPHEUS JAPONICUS MIERS

As a consequence of global climate change in the last two centuries, ocean acidification resulted in warming waters.In this study, we explored the physiological responses of snapping shrimp Alpheus japonicus Miers, an abundant and broadly distributed species of marine crustacean, to modern (400ppm) and future ambient CO_2 (2000ppm) seawater acidic levels at two temperatures (20, 23°C) in experiment.Results show that the elevated acidic and thermal stresses led to oxidative stress, and acidic and thermal stresses had interactive effects on the catalase (CAT) activity, glutathione (GSH) content and the ratio of glutathione (GSH) to oxidized glutathione (GSSG), GSH content and the GSH/GSSG ratio of the shrimp were significantly inhibited by 66.0% and 20.8%, respectively, while the LPO level increased by 51.4% in contrast to the controls.Moreover, the HK activity was increased and PROT content reduced, showing disturbance in glycolysis and energy reserves.In other words, the energy metabolism strategy adopted by A.japonicus would be not sustainable in the long term.In addition, the short-term seawater acidification and elevated thermal stress in the experiment triggered oxidative stress and disturbance in energy metabolism of A.japonicus.Therefore, global warming and ocean acidification would affect the population replenishment of the species, as well as other ocean creatures in the future

一种部分解耦并联机构运动学与动力学分析

针对并联机床加工复杂曲面零件，设计了一种部分解耦5自由度并联机构。分析了该并联机构的自由度和解耦性能；基于矢量法，推导了机构中各驱动分支和动平台运动之间的映射关系，分析了并联机构中各构件的运动；考虑各构件的重力、惯性力和惯性力矩，利用虚功原理建立了机构的动力学方程；利用CAD软件对并联机构运动学和动力学方程进行了验证。分析了部分解耦并联机构的奇异性，为该类机构的应用奠定了理论基础

四种救护陆龟粪便菌群组成分析

为了解不同陆龟的肠道微生物菌群的区系特点,采用高通量测序技术,分析比较人工养殖的缅甸陆龟(Indotestudo elongata)、苏卡达陆龟(Centrochelys sulcata)、辐射陆龟(Astrochelys radiata)和豹龟(Stigmochelys pardalis)粪便微生物多样性。结果表明:厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、互养菌门(Synergistetes)和变形菌门(Proteobacteria)均为4种陆龟的优势菌门,与其他已报告的爬行动物优势菌门相似。虽然陆龟属于后肠发酵动物,但4种陆龟粪便样本中相对丰度排名前10的菌属(普雷沃氏菌属Prevotella、瘤胃球菌属Ruminococcus、梭菌属Clostridium等)与反刍动物瘤胃菌群有着极为明显的相似性。NMDS分析结果显示来自相似环境的龟粪便微生物差异更小,LEfSe分析显示每种龟都有着各自的标志物菌,因此导致肠道菌群差异的根本因素很大程度上取决于自身的遗传特质。氨基酸球菌属(Acidaminococcus)和费氏刺骨鱼菌属(Epulopiscium)在已报导的爬行动物肠道微生物中未见提及,但在4种陆龟中均被检测到,且个别陆龟粪便样本中相对丰度接近20%

不同加工方式的油茶籽油对变应性接触性 皮炎的抗炎效果Anti-inflammatory effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis

为了明确不同加工方式的油茶籽油对变应性接触性皮炎（ACD）的治疗效果，并探究油茶籽油中的主要抗炎物质，分别制备低温压榨油茶籽油、热榨油茶籽原油、热榨精炼油茶籽油、土榨油茶籽油和超临界CO2油茶籽油，分析不同加工方式及添加不同活性物质的油茶籽油对ACD模型小鼠耳廓肿胀的影响，并结合ELISA法、HE染色和免疫组化探究其对小鼠炎症的抑制效果。结果表明：超临界CO2油茶籽油对ACD小鼠耳廓肿胀的抑制率最高，与阳性对照组相比其小鼠血清中炎症因子TNF-α、IL-1β和IL-6含量降低率超过10%，炎症细胞浸润程度明显好转，耳廓肿胀组织中NF-κB阳性表达量与阴性对照组相比无显著差异；预防性给药的抗炎效果普遍低于造模后给药；倍数添加角鲨烯和生育酚的油茶籽油对ACD小鼠耳廓肿胀的抑制率显著提高，在50%左右，且角鲨烯、生育酚处理组小鼠耳廓组织中NF-κB阳性表达量与阴性对照组无显著差异。因此，外用超临界CO2萃取的油茶籽油治疗小鼠ACD效果最佳，其抗炎效果并非源于单一活性成分，与角鲨烯和生育酚等活性成分均密切相关。In order to clarify the therapeutic effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis (ACD), and further explore the potential active substances of anti-inflammatory in oil-tea camellia seed oil, cold pressed oil-tea camellia seed oil, hot pressed crude oil-tea camellia seed oil, hot pressed-refined oil-tea camellia seed oil, native pressed oil-tea camellia seed oil, and supercritical CO2 extracted oil-tea camellia seed oil were prepared, the effects of different processing methods of oil-tea camellia seed oils and different active substances added with oil-tea camellia seed oils on auricular swelling in ACD model mice were analyzed, and their anti-inflammatory effect in mice were studied by combining ELISA method, HE staining, and immunohistochemistry. The results showed that the supercritical CO2 extracted oil-tea camellia seed oil showed the highest inhibition rate on auricular swelling of ACD mice, and the inflammatory factors, such as TNF-α, IL-1β and IL-6 were reduced more than 10% compared with positive control. The degree of inflammatory infiltration significantly improved, and there was no significant difference in NF-κB positive expression of auricular tissue between supercritical CO2 extracted oil-tea camellia seed oil treatment group and negative control. However, the anti-inflammatory effect of prophylactic administration was generally lower than that of post modeling administration.The oil-tea camellia seed oil that supplemented with multiple squalene and tocopherol increased the inhibition rate of auricular swelling about 50%. There was no significant difference in NF-κB positive expression of auricular tissue between the squalene and tocopherol group and the negative control. In general, supercritical CO2 extracted oil-tea camellia seed oil exhibits the best therapeutic effect on ACD mice, and the active anti-inflammatory ingredients are closely related to squalene and tocopherol