溶菌酶1在小鼠超数排卵前后孵化及休眠囊胚中差异表达的研究

目的研究溶菌酶1(lysozyme 1,LYZ1)基因在超数排卵前后小鼠孵化囊胚和休眠胚胎中的分布以及表达,探究动物胚胎着床过程中新的调节机制。方法从妊娠5 d ICR小鼠体内获取的正常孵化囊胚和超排囊胚,利用小鼠延迟着床模型于妊娠第8天获取休眠胚胎和超排休眠胚胎。利用免疫荧光和Western Blot方法检测LYZ1蛋白在四组胚胎中的分布和差异表达变化。结果 LYZ1在超数排卵前、后小鼠孵化囊胚和休眠胚胎中均有表达,且主要集中在内细胞团中,滋养层细胞和胞质中少见分布;与未进行超排的小鼠相比,LYZ1蛋白在超排后小鼠胚胎中表达量显著上调,与未营造休眠模型的小鼠相比,LYZ1蛋白在休眠模型小鼠胚胎中的表达量显著上调。结论 LYZ1蛋白在囊胚内细胞团中表达,可能参与调节胚胎内细胞团的发育;LYZ1蛋白在超排-休眠胚胎中的高表达,说明LYZ1蛋白在休眠和超数排卵的双重影响下,会因为抵御不利环境而上调。2016年度科技创新服务能力建设-科技计划重点项目(KZ201610020018);;2018科技创新服务能力建设(KZ201610020018)~

福建鲍产业发展形势分析

鲍,素有\"软黄金\"的美誉。自上世纪90年代开始,福建引入皱纹盘鲍与日本盘鲍的杂交鲍并逐渐扩大养殖规模。笔者归纳了福建省鲍的养殖分布及生产情况,结合鲍的消费结构及产业支撑现状发现鲍产业发展过程中的育苗场规划,养殖管理,种质退化,安全用药等方面存在问题,对此提出推进科学养殖,增强环保意识;加强管理制度建设,实现质量监管;加强种质管理,选育新品种;加强技术体系建设;提升产业融合度等建议

重振雄风——卧龙圈养大熊猫的“性教育”

一、阴盛阳衰——圈养雄性大熊猫繁殖力极为低下自从1963年世界上第一只人工繁殖的大熊猫诞生以来,圈养大熊猫的人工繁殖一直面临着三大难关：配种难、育幼难和受孕难。一直以来有繁殖能力的雄性大熊猫低于雌性。人工饲养的大熊猫有80%以上的雄体丧失了交配能力

Research and developments of electrocatalysts and membranes for proton exchange membrane fuel cells

The research progress in DICP of the electrocatalysts and membranes for proton exchange membrane (PEM) fuel cells will be presented here.The research progress in DICP of the electrocatalysts and membranes for proton exchange membrane (PEM) fuel cells will be presented here

Nitrogen-doped carbon Electrocatalysts for Oxygen Reduction Reaction in Proton Exchange Membrane Fuel Cells

Fuel Cell(FCs) technology, as a new renewable energy generation, has garnered great interest and attention around the world in the past decade because of its advantages such as high power density, environment friendly and so on. Besides, it has the potential to strengthen the national energy security by reducing dependence on petroleum products and offering a cleaner, more efficient alternatives of today energy technology.Fuel Cell(FCs) technology, as a new renewable energy generation, has garnered great interest and attention around the world in the past decade because of its advantages such as high power density, environment friendly and so on. Besides, it has the potential to strengthen the national energy security by reducing dependence on petroleum products and offering a cleaner, more efficient alternatives of today energy technology

Oxygen Reduction Reaction on Carbon Supported Ruthenium-based Electrocatalysts in PEMFC

The ruthenium-based electrocatalysts supported on carbon black were prepared by the decarbonylation of the transition metal carbonyl with the 1,6-hexanediol as the solvent. The catalysts are characterized via X-ray diffraction (XRD), transmission electron microscopy(TEM) and high resolution TEM(HRTEM). The electrochemical behaviours of the catalysts were investigated by cyclic voltammetry(CV)and rotating disk eletrode(RDE) measurements in 0.5 M H2SO4 solution. The catalysts demonstrate attractive catalytic activity towards the ORR. The catalyst is expected to be promising alternative non-Pt electrocatalysts for PEMFC

一种燃料电池用阴极催化剂及制备方法

本发明涉及一种燃料电池用阴极催化剂及制备方法，所述催化剂为空壳型碳担载Pt基纳米催化剂，以Pt或Pt与过渡金属组成的合金为活性组分，其通式为Pt或PtMx，其中，M=Ag、Au、Ru、Rh、Pd、Os、或Ir，0.05≤x≤0.95，催化剂粒径为10-100nm，壳体壁的厚度1-20nm。制备的催化剂Pt基组分利用率高，相比于传统的碳载Pt基纳米颗粒催化剂，本发明的催化剂在保证表面有效Pt基活性组分的前提下，省去传统纳米颗粒内部不参与反应的Pt基组分，提高Pt基组分的利用率；同时空壳结构诱导Pt基组分发生晶格畸变，产生电子调控作用，参与催化反应的Pt基组分催化活性高