Concentration Protile of Etchant Measured by Microelectrode Technique in the Process of Chemical Micromachining

利用微圆盘电极技术,测定了KBr、L-胱氨酸和硫酸组成的刻蚀溶液体系中Pt电极表面电化学氧化产生的刻蚀剂Br2浓度分布,为约束刻蚀剂层技术(CELT)中刻蚀体系的选择和优化提供更直观的依据.GaAs表面CELT微加工实验证明了用微圆盘电极测得的表面刻蚀剂的浓度分布趋势与微加工实验所得到的结果一致.A carbon-disk microelectrode was used to investigate the surface concentration profile of etchant Br2, which was electrogenerated on the Pt working electrode. The steady state reducing currents of Br2 at different distances away from the Pt electode was measured. The concentration profile was estimated from the current-distance variation curves as a function of different sampling times. Experimentally determined concentration profiles are in good agreement with those estimated from the microetching results. The microelectrode technique has offered a good method to choose suitable etching solution for chemical micromachining.国家高新技术发展规划(863项目)(2002AA404170);; 福建省自然科学基金计划资助项目(E0520001

Concentration protile of etchant measured by microelectrode technique in the process of chemical micromachining

A carbon-disk microelectrode was used to investigate the surface concentration profile of etchant Br-2, which was electrogenerated on the Pt working electrode. The steady state reducing currents of Br-2 at different distances away from the Pt electode was measured. The concentration profile was estimated from the current-distance variation curves as a function of different sampling times. Experimentally determined concentration profiles are in good agreement with those estimated from the microetching results. The microelectrode technique has offered a good method to choose suitable etching solution for chemical micromachining

Identification and preliminary analysis of a novel full-length cDNA encoding retinoid X receptor 2 from Schistosoma japonicum

通信作者 E-mail: [email protected][中文文摘]目的克隆编码日本血吸虫视黄酸X受体2(SjRXR2)蛋白的全长cDNA,并对其进行初步研究。方法利用cDNA末端快速扩增技术(RACE)获得SjRXR2蛋白全长编码cDNA。利用生物信息学技术,对基因结构进行初步分析。利用实时荧光定量(Real time)PCR技术对该基因在日本血吸虫不同时期虫体中的转录情况进行分析。应用在线抗体表位预测软件获得SjRXR2配体结合区抗原性较强的一个多肽序列,合成该多肽片段,并免疫小鼠制备抗血清。利用Western blot技术分析该蛋白在日本血吸虫中的表达。结果采用RACE技术成功获得了SjRXR2蛋白全长编码cDNA,总长度为5 960bp,其完整开放阅读框为4 308 bp,编码1 435个氨基酸,预测分子量为159 kDa。生物信息学分析表明该基因编码的蛋白质序列具有核受体家族2的典型结构域特征,且与曼氏血吸虫RXR2有较高的相似性。Real time PCR分析表明,该基因在21、42 d龄日本血吸虫虫体内有较高的转录水平。Western blot分析表明,小鼠SjRXR2多肽免疫血清可特异性识别日本血吸虫虫体150 kDa蛋白。结论成功获得了编码SjRXR2蛋白的全长 cDNA，并制备了针对该蛋白的特异性多克隆抗体，为进一步研究该蛋白的功能奠定了基础。 .国家自然科学基金（31172315）；公益性行业（农业）科研专项（20090303036

海洋酸化对二甲基硫生产释放影响的研究进展

Since the industrial revolution, huge amounts of CO2 released by human beings has been absorbed by the ocean, which has caused decreased of pH and calcium carbonate saturation in the ocean, resulting in ocean acidification. Ocean acidification can not only affect the ocean primary productivity, growth, reproduction and metabolism of marine organisms, but also affect the production and release of dimethylsulfide (DMS). DMS mainly produced via the degradation of dimethylsulfoniopropionate (DMSP) and the results on the influence of ocean acidification on DMS(P) production isstill controversy. Therefore, to accurately predict the future trend of DMS (P) under ocean acidification stress,it is necessary to explore the migration and transformation processes of DMS(P) under different pCO2 ocean acidification

Left-Right hand distinction for multi-touch tabletop interaction

现有多触点交互桌面系统通常只提供触点位置、形状等信息,不包括触点左右手归属信息.触点所属手及左右手手性信息的提供,对于多指手势识别、丰富双手交互 技术,尤其是非对称双手交互技术具有重要意义.基于手的解剖结构特征,提出一种不需辅助硬件设备的触点左右手归属判定方法.首先,以手势设计的基本原则为 指导,根据手的解剖结构特征,提出交互桌面手-臂系统三角形模型;其次,基于该三角形模型,给出多触点交互桌面同手触点聚类方法及左右手识别方法;然后, 对MTDriver做扩展,实现了提供左右手信息的多触点跟踪工具箱,并提出了基于左右手信息的交互桌面交互技术;最后,评估结果表明,同手触点聚类方法 及触点左右手手性识别方法均具有较高的正确率,而且在时间性能方面满足交互桌面交互实时性要求.In a multi-touch interactive system, distinguishing between user's left and right hands is of great significance for recognizing multi-finger gestures and further fully exploring the potential of bimanual interaction. However, the function of left-right hand distinction is beyond the capability of most multi-touch systems. In this paper, a robust solution to the left-right hand distinction based on the anatomical structure characteristic of hand is presented. Firstly, guided by the basic principles of gesture designing, the interactive tabletop hand-arm triangle model is proposed based on the anatomical structure characteristic of hand. Secondly, multi-touch interactive tabletop same hand contact points clustering method and left-right hand recognizing method are given based on the triangle model. Then, the methods are integrated into MTDriver to detect and send contact points's left-right information to the multi-touch applications and new bimanual interactive technologies are demonstrated. Finally, evaluation shows that the solution can achieve high recognition accuracy and good time performance that could support fluency bimanual interaction on interactive tabletop. © Copyright 2014, Institute of Software, the Chinese Academy of Sciences. All rights reserved

Research progress of nuclear hormone receptors in Schistosoma

核受体在后生动物中作为一类重要的转录调控因子,在一系列重要的生化过程中发挥重要的作用,包括细胞分化和增殖。核受体含共有的蛋白结构,包含一个n端-A/b区,一个高度保守的dbd,一个铰链区(d区)和一个C-端lbd区。到目前为止,寄生扁形动物中的核受体,只在曼氏血吸虫和日本血吸虫中有报道。本文对近年来血吸虫的核受体的研究成果做简要综述,以为今后更进一步的研究提供参考。Nuclear hormone receptors(NRs)belong to a large protein superfamily that are important transcriptional modulator in metazoan and play vital role in metabolism including cell differentiation and proliferation.NRs share a common tertiary structure,the typical NR contains an N-terminal A/B domain,a highly conserved DNA binding domain(DBD),a hinge(D domain),and a C-terminal ligand binding domain(LBD).The nuclear receptors in parasitic platyhelminths were reported so far only in the Schistosoma mansoni and Schistosoma japonicum.The aim of this review is to examine the current knowledge of nuclear receptors in parasitic platyhelminths that provide the reference for further research.国家自然科学基金(No.31172315;No.81271871)资助~

Lactobacillus pentosus P307, its application and process for preparing bacteriocin

本发明公开了一株戊糖乳杆菌P307及其应用和利用其制备细菌素的方法，属于微生物技术领域。该戊糖乳杆菌P307是从刺参内脏发酵物中分离、纯化得到的，能够适应低盐环境，并且发酵代谢产生细菌素，其已保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20221055。经研究发现，该戊糖乳杆菌P307对革兰氏阳性菌和革兰氏阴性菌均有明显的抑制效果，抑菌谱较广泛，可以应用在果蔬抗氧化保鲜和水产品防腐保鲜中；其产生的细菌素具有良好的热稳定性及pH稳定性，适用范围广，并且具有显著的蛋白酶敏感性，经过胃蛋白酶等酶处理后几乎完全失去抑菌活性，避免了在体内的负面影响