Induction of MET Receptor Tyrosine Kinase Down-Regulation through Antibody-Mediated Receptor Clustering

The proto-oncoprotein MET is a receptor tyrosine kinase that plays a key role in cancer cell growth and invasion. We have used fluorescence-tagged antibodies to activate MET in live serum-starved glioblastoma cells and monitor the fate of antibody-bound MET receptor in single cell-based assays. We found that the antibodies induced rapid and transient formation of highly polarized MET clusters on the plasma membrane and promoted the activation of MET, resembling the initial effects of binding to its ligand, HGF. However, the antibody-induced clustering and activation of MET led to the rapid removal of the receptor from cell surface and altered its intracellular processing, resulted in rapid degradation of the receptor. Consequently, while cells pre-treated with HGF remain competent to respond to further HGF stimulation, cells pre-treated with antibodies are refractory to further HGF stimulation due to antibody-mediated MET depletion. Removal of MET by sustained treatment of antibodies blocked cancer cell migration and invasion. Our studies reveal a novel mechanism to alter the recycling process of MET in glioblastoma cancer cells by promoting the receptor degradation through a proteasome-sensitive and lysosome-dependent pathway through the ligand-independent activation of MET using anti-MET antibodies

Magnetothermoelectric DC conductivities from holography models with hyperscaling factor in Lifshitz spacetime

We investigate an Einstein-Maxwell-Dilaton-Axion holographic model and obtain two branches of a charged black hole solution with a dynamic exponent and a hyperscaling violation factor when a magnetic field presents. The magnetothermoelectric DC conductivities are then calculated in terms of horizon data by means of holographic principle. We find that linear temperature dependence resistivity and quadratic temperature dependence inverse Hall angle can be achieved in our model. The well-known anomalous temperature scaling of the Nernst signal and the Seebeck coefficient of cuprate strange metals are also discussed.Comment: 1+23 pages, 4 figures, references adde

Electroweak Corrections to the Decays of Stop and Gluino

The electroweak corrections at the order of $O(\alpha_{ew}m_{t(b)}^2/m_W^2)$ to the partial widths of the $\tilde{t}_2 \to \tilde{g} + t$ and $\tilde{g} \to \tilde{t}_1 + \bar{t}$ decays (depending on the masses of the particles involved) are investigated within the Supersymmetric Standard Model. The relative corrections can reach the value of 10% in some parameter space, which can be comparable with the corresponding QCD corrections. Therefore, they should be taken into account for the precise experimental measurement at future colliders.Comment: 15 pages, 5 figures, Physical Review D publication versio