Large Culures for Haematococcus pluvialis Outside and Accumulation of Astaxanthin

针对雨生红球藻(Haematococcus pluvialis)大体积室外培养开展实验,对不同品系藻株、不同营养方式、胁迫条件等对雨生红球藻生长及虾青素积累的进行了初步研究.结果表明,HPM品系在室外培养过程中指数生长率为0.22,胁迫后虾青素产率为1.35mg/L,均优于其他两个品系.室外培养中加入醋酸钠的混合培养基可以缩短胁迫时间,并且其虾青素的积累量比光合自养培养下提高了126.3%.盐胁迫比高光胁迫更能有效地积累虾青素,虾青素产率从0.76 mg/L提高到了1.33 mg/L.The green microalga Haematococcus pluvialis was cultured outside in large volume,the effects of different trophic and stress conditions on cell growth and astaxanthin accumulation were studied.The effects of different strains on accumulating astaxanthin was also investigated.The results showed that the growth rate of the strain HPM outside was 0.22,and the astaxanthin production was 1.35 mg/L,which were higher than the other two strains.The stress period was shortened under the mixotrophic system with addition of NaAc,and the astaxanthin content moreased 126.3% than that under the autotrophic system.The stress of high salinity was better to accumulate astaxanthin than high light intensity.Under the stress of high salinity,the astaxanthin production was 1.33 mg/L compared to the data under the stress of high light(0.76 mg/L).厦门市科技项目(3052Z20031086)资