The Phylogeny Tree Reconstruction Based on the Usage Frequencyof Codons and Corresponding Complementary Codons

为了更深入地了解密码子与互补密码子使用相关的内涵,70个单细胞和多细胞生物被更进一步的分析。结果得到70种细胞生物具有较高的相关系数平均值(0.5101,标准差是0.1389),表明密码子与其互补密码子使用间确实存在正的使用相关联系,进一步地支持了以前的结果。此外,通过对70个单细胞和多细胞生物在基于密码子与互补密码子使用频率差异,以及配对频率的高低信息的分析基础上,绘制进化树,得到的进化树与传统的进化树非常接近。The hypothesis that a codon bias usage is identical to its complementary codon usage preference has been investigated by using the relationship analysis of codons vs their complementary ones among 70 organisms. Significantly positive usage correlations between codons and their complementary ones were found and its implication in biology was also analyzed. The codoncomplementary codon tree was further built, which fairly exhibited the evolutionary relationship of these organisms. The results not only demonstrated the validity of our hypothesis,but also manifested the usefulness of correlation analysis in studying on codon usage pattern and molecular evolutionary mechanisms of organism.ThisworkwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina(No.60305001)andagrantfromtheScienceandTechnologyMinistryofChina(PreliminaryStudyonFunctionalGenomeSystematics)(No.2001CCA01400)

基于拓扑链路识别的光网络流量数据合成算法

基于深度学习的光网络流量诊断与预测等场景中，由于保密等原因，光链路的流量数据采集和存储工作受限。针对数据量少而无法支撑深度学习的问题，文章提出了一种基于拓扑链路识别的光网络流量数据合成算法，其核心思想是在生成对抗网络框架下，联合基于光网络拓扑的条件生成模型和基于光网络流量的数据合成模型，以自监督的方式合成指定光链路的流量数据。仿真结果表明，所提算法合成的光网络流量数据在自相关系数指标上与真实数据接近且使得基于全连接神经网络的流量预测模型准确率达到95%以上

中国燃料乙醇的应用及生产技术的效益分析与评价

针对国内外燃料乙醇的应用和发展情况，对比分析了淀粉质、糖质和纤维素类原料燃料乙醇生产工艺的异同，并对我国3种原料的资源状况进行了评价。以10万t/a的燃料乙醇工厂为例，对不同原料燃料乙醇生产工艺的经济性、能源效益和社会效益进行了对比分析，发现甜高梁和木薯最有经济优势；从能源效益分析可知生产工艺能耗约占80％，工艺改进非常重要；从单位土地能源净产出情况看，甜高梁最高，其次是甘蔗和木薯，可见能源作物具有开发潜力；通过社会效益分析，10万t燃料乙醇工厂会给农民带来2～4亿元的年收益，提供约1000个的就业岗位；且10万t燃料乙醇的应用CO2年减排量可达30万t以上，减排CO、CH等汽车有害污染物近1700t

Expressions of Fas/DcR3 and FasL/RGD-FasL mediated apoptosis in pituitary adenoma cells

目的研究RGD-FasL诱导垂体腺瘤细胞株GH3、MMQ、AtT20所产生的细胞毒性效应,并探讨其机制。方法应用RT-PCR法和流式细胞仪检测肿瘤细胞上Fas、DcR3的表达。应用MTT测定法检测FasL、RGD-FasL对肿瘤细胞所产生的细胞毒性效应,并经琼脂糖凝胶电泳证实是否为该配体所诱导的凋亡。通过流式细胞仪PI染色法评价细胞周期的变化和凋亡分析。通过Western蛋白印迹法检测Caspase 8、Caspase 9、Caspase 3、Bcl-2、RANKL和JNK2的表达。结果垂体腺瘤细胞株GH3、MMQ、AtT20均表达Fas和DcR3。FasL和RGD-FasL诱导肿瘤细胞所产生的细胞毒性效应均呈现剂量依赖关系。细胞周期分析表明RGD-FasL能诱导细胞周期的停滞。经RGD-FasL或FasL干预的肿瘤细胞的凋亡指数差异不明显。经RGD-FasL干预后,Caspase 8、Caspase 9、Caspase 3、RANKL和JNK2的表达均增加,而Bcl-2的表达减少。结论RGD-FasL能通过Caspase途径诱导垂体腺瘤细胞的凋亡。RGD-FasL的研制很可能为垂体腺瘤的生物靶向治疗提供一种新的途径。Objective To investigate the cytotoxic effects of pituitary adenoma cell lines GH3,MMQ,AtT20 induced by RGD-FasL and the underlying mechanism.Methods Fas and DcR3 mRNAs were detected by RT-PCR and their surface expression was measured by flow cytometry.Cytotoxicity exerted by RGD-FasL on tumor cells was measured with MTT assay and the induced apoptosis was determined by agarose gel electrophoresis.The cell cycle and apoptosis was assessed by flow cytometry with PI staining.The expressions of Caspase8,Caspase9,Caspase3,Bcl-2,RANKL and JNK2 were detected by Western Blot.Results GH3,MMQ and AtT20 cells expressed Fas and DcR3.The cytotoxic effects of FasL and RGD-FasL on tumor cells were dose-dependent.The cell cycle analysis indicated that RGD-FasL could induce cell cycle stagnation.In pituitary tumor cells treated with RGD-FasL,the apoptostic index was insignificantly different from that treated with FasL.The expression of Caspase8,Caspase9,Caspase3,RANKL and JNK2 increased while that of Bcl-2 decreased after treated with RGD-FasL.Conclusion RGD-FasL induces apoptosis of pituitary adenoma cells through Caspase activation,and may be a novel therapeutical candidate for the treatment of pituitary adenomas