A preliminary approach to biological function and application of Recombinant Human Prothymosin α

胸腺素α原(Prothymosinα，ProTα)于1984年首次从大鼠胸腺中分离出来，当时被认为是一种胸腺激素，后来发现这种蛋白普遍存在于所有哺乳动物组织中，人ProTα分子量约为12.4kD，由109个氨基酸构成，其蛋白序列富含大量酸性氨基酸，是一种高度酸性的亲水性多肽，等电点为3.5，蛋白结构是一种罕见的非折叠的“天然无结构蛋白”，带负电，免疫原性极差，具有核内定位信号，进化上高度保守，分布极其广泛，淋巴组织及非淋巴组织均可找到，基因定位于第2号染色体上，是哺乳动物中唯一被报道过带有磷酸化谷氨酸的蛋白。基因文库检测中显示人ProTα基因是细胞中表达最丰富的基因之一。 ProTα大量存在...Prothymosin α (ProTα) was intitally isolated from rat thymus, highly conserved among mammals. Human ProTα was a 109 amino acid residue polypetide, and its molecular weight was 12.4 kD. 3.5 of isoelectric point values, rich in glutamic acid. The structure of ProTα was an intrinsically disordered protein which structure was unfolded, with nuclear location signal, electrophorese to positive pole, a h...学位：理学硕士院系专业：环境与生态学院_生态学学号：3312012115166

The Research Progress of Camel Milk in Protection and Treatment of 2 Type Diabetes and its Application Prospect in China

骆驼奶含有丰富的营养物质,尤其是胰岛素及胰岛素肽类物质。文章研究目前糖尿病的流行状况和危害,分析目前骆驼奶在防治糖尿病的研究现状及其应用前景。Camel milk was rich in nutrition, with high level of insulin or insulin protein.The paper summari-zes the diabetes prevalence and harm in human being, and introduces the research progress of camel milk in protec-tion and treatment of diabetes and its application prospect in China.新疆维吾尔自治区科技支疆项目(201191163)资

Study on the Localization of Prothymosinαin Normal and Tumor Tissues

检测并分析胸腺素α原(PrOTHyMOSInα,PrOTα)在小鼠正常肝组织与肝肿瘤组织中表达、分布的情况.取20只昆明雄性小鼠随机分为对照组和移植性肝癌模型组,每组10只,模型组接种H22肝癌组织,建立移植性肝癌模型.7d后将模型组肝癌组织和对照组正常肝的组织取出,部分组织按常规石蜡包埋方法制片,免疫组织化学法分别检测肝癌组织与正常肝组织中PrOTα的表达分布情况;部分组织按提取胞质蛋白的方法获得蛋白用于WESTErn-blOT分析.在WESTErnblOT中,PrOTα在肝癌组织细胞质的表达量明显低于正常肝组织;在免疫组织化学法中,正常肝组织的细胞核呈阴性,胞质呈强阳性;而在肝癌组织中胞核呈强阳性,胞质呈弱阳性.PrOTα在正常组织与肿瘤组织中的表达分布有明显差异.由于在WESTErn-blOT中所提的蛋白主要为胞质蛋白,在癌变的组织中,PrOTα主要集中在细胞核中,因此推测,当胞质中的PrOTα大量表达时对于肝细胞稳定、抑制细胞发生癌变有重要功能,此结果为进一步研究PrOTα在肿瘤发生和诊断中的作用提供了重要的理论依据.To study and analyze the expression and distribution of prothymosinα(ProTα)in normal and tumor cells of mice,twenty male Kunming mice were randomly divided into normal group and model group.Mice in the model group were inoculated with H22(Hepatoma 22)tumor cell.After 7days,part of livers of the normal and hepatocellular carcinoma tissues were extracted to make slices for immunohistochemistry test and HE staining of ProTα,while part of tissues were homogenized,and cell lysates were extracted for Western-blot to test the expression level of ProTαin normal liver and hepatocellular carcinoma tissues.The results showed that the expression level of ProTαin the cytoplasm of normal liver cell was significantly higher than that of hepatocellular carcinoma tissue.Immunohistochemistry showed the strong positive reaction in the cytoplasm of normal tissue,and negative in the nuclei.On the contrary,hepatocellular carcinoma tissue showed strong positive immunoreactivity in the nuclei,but weak positive response in cytoplasm.The results indicated that the distribution of ProTαin the normal and tumor tissues was changeable and nuclei translocation of ProTαgradually took place during the occurrence and development of hepatocellular carcinoma.These results laid the foundation for the mechanism study of ProTαin tumor occurrence,and would be beneficial to develop diagnosis method of cancer.福建省自然科学基金(2013J01383