Advantages of double-embedding with agar and paraffin in making light microscope slices of small vessels

预先将固定好的小血管包埋到1.5%琼脂中,调整好其位置和管腔。采用自制的琼脂包埋模子可以方便快捷的获得结构完整大小适中的组织琼脂块。再按照常规的的脱水包埋程序做石蜡包埋,最后切片染色。结果显示,琼脂石蜡双包埋法保持了小血管管腔的完整性和规则性,内膜等组织结构几乎没有损坏和丢失,最终得到较为理想的光镜切片。The fixed small vessels were pre-embedded into 1.5% agar in advance,and its position and lumen were adjusted well.By using self-made agarembedding mould,the agar block in complete structure and right size could be obtained conveniently.Then paraffin embedding was done according to the conventional dehydration and embedding procedure,and staining was the last step.The results indicated that,by using the double embedding method,the integrity of lumen was kept,the intima and other tissues of vessel were not damaged and lost,the ideal light microscope slices were gotten ultimately.国家重点基础研究发展规划项目资助(973计划项目)(编号:2012CBA01303