A Research on the Attraction of World Cultural Heritage Based on Tourist Perception:A Case Study of Mountain Tai

本研究以泰山为例,通过对旅游者进行问卷调查,利用因子分析方法,确定了文化遗产旅游吸引力系统的主要构成因素及其内部结构。依据因子的特征值,吸引力因素可以分为不同的层次,其中核心吸引力因素决定着文化遗产旅游吸引力的性质。遗产类资源是一个复杂的文化系统,旅游吸引力系统的构成因素只是整体文化系统的组成部分,但代表了旅游者感知的文化遗产特征,发展旅游应注意开发项目与吸引力系统特性的一致性。By taking Mountain Tai as an example,this research did a questionnaire investigation of the travelers,applied factor analysis method to determine the main components of the attraction system of cultural heritage tours and its internal structure.The attraction factors are grouped into different levels in accordance with their trait values.Among these factors,the core factors determine the nature of the attraction of cultural heritage tours.The authors believe that heritage resources is composed of a complex cultural system in which the components of the attraction system play only a minor part,but represent the cultural heritage traits perceived by tourists,and that tourism development projects should be programed in line with the specialty of the attraction system.山东省社会科学规划研究基地重点项目(批准号:05JDW13)阶段性成

Expression and Localization of hnRNP A2/B1 during Differentiation of Human Osteosarcoma MG-63 Cells Induced by HMBA

背景与目的:核基质蛋白的差异表达与细胞癌变和增殖分化调控关系密切。本研究观察了hnRNP A2/B1在诱导分化处理前后人成骨肉瘤MG-63细胞核基质中的存在和分布,及其与Actin、Prohibitin的共定位关系。方法:选择性抽提经环六亚甲基双乙酰胺(hexamethylene bisacetamide,HMBA)诱导处理前后的MG-63细胞核基质,并运用双向电泳、质谱分析、蛋白质印记杂交、免疫荧光、激光共聚焦等技术检测hnRNP A2/B1在核基质中的表达与定位变化,及其与相关蛋白的共定位关系。结果:双向电泳及蛋白质印迹杂交结果证实了hnRNP A2/B1存在于MG-63细胞核基质蛋白组分中,并在HMBA处理后细胞核基质中表达下调;免疫荧光显微镜观察显示hnRNP A2/B1定位在核基质上,经HMBA处理后hnRNP A2/B1表达减弱。激光共聚焦显微镜观察结果显示,hnRNP A2/B1与细胞核基质蛋白组分Actin、细胞增殖相关调控因子Prohibitin具有共定位关系,但在诱导处理后细胞内的共定位关系减弱。结论:hnRNP A2/B1在MG-63细胞诱导分化过程中的表达分布,及其与Actin、Prohibitin的共定位关系的改变对MG-63细胞分化具有重要影响,值得进一步探索和研究。BACKGROUND & OBJECTIVE: The differentially expressed nuclear matrix proteins have great effects on canceration and regulation of cell differentiation. This study was to explore the existence and distribution of ribonucleoprotein hnRNP A2/B1 in nuclear matrix and its co-localization with Actin and Prohibitin in human osteosarcoma MG-63 cells before and after hexamethylene bisacetamide (HMBA) treatment. METHODS: The nuclear matrix of MG-63 cells before and after treatment of HMBA were selectively extracted. The expression and localization of hnRNP A2/B1 in nuclear matrix were detected by 2-D PAGE, MALDI-TOF-MS, Western blot, and immunofluorescent staining. The co-localization of hnRNP A2/B1 with Actin and Prohibitin was observed under laser scanning confocal microscope (LSCM). RESULTS: hnRNP A2/B1 was detected in the component of nuclear matrix proteins of MG-63 cells by Western blot and immunogold staining and its expression was decreased after treatment of HMBA. hnRNP A2/B1 was located in the nuclear matrix, and its expression was weakened after HMBA treatment. hnRNP A2/B1 was co-localized with Actin or Prohibitin in MG-63 cells, while the co-localization relationship was weakened during differentiation of MG-63 cells. CONCLUSIONS: hnRNP A2/B1 is a kind of nuclear matrix protein, and localizes in the nuclear matrix. The distribution and expression of hnRNP A2/B1 and its co-localization with Actin and Prohibitin play important roles during the differentiation of MG-63 cells.国家自然科学基金项目(No.30470877)~

DD-MZM单边带调制系统偏置电压优化控制方案

为了获得更加稳定的梳状谱发生器,对基于DD（双平行）-MZM（马赫-曾德尔调制器）的单边带调制系统偏置电压控制方案进行了研究,着重分析了非理想消光比对MZM以及调制器偏置电压控制标准的影响,并在此基础上提出了优化的偏置电压判别标准。研究证明,在非理想消光比条件下,选取MZM传输曲线的最高点作为系统偏置电压的判别标准具有更高的精确性

草甸沼泽土壤硝化-反硝化作用和有机碳矿化对氮输入的响应

通过室内培养实验,研究了草甸沼泽土壤N2O排放和反硝化损失对氮输入的响应特征,结果表明,在培养期(23 d)内N2O平均排放速率为0.32(N0),0.87(N1),17.69(N2),28.07(N3)μgN2O-N/(kg±.h),反硝化平均损失速率为0.25(N0),0.81(N1),22.29(N2),30.28(N3)μgN2O-N/(kg±.h),两者都随氮输入量增高而升高。其中,N3处理N2O平均排放速率和反硝化平均损失速率与对照差异显著(p<0.05),N1和N2与对照差异不显著。N2O排放总量占氮输入的比例为0.03%(N1),1.04%(N2),1.76%(N3),反硝化损失总量占氮输入的比例为0.04%(N1),1.29%(N2),1.93%(N3),均表现为随氮输入量的增大而增高。N1处理下有机碳矿化速率低于对照,而N2和N3有机碳矿化速率高于对照,说明低氮输入对有机碳矿化有一定抑制作用,高氮输入促进有机碳矿化