Construction of expression vector of HCV NS4B and its effect on cell apoptosis

目的 构建丙型肝炎病毒（HCV）2a型非结构蛋白4B（NS4B）的真核表达载体,并观察其对骨肉瘤细胞U2OS凋亡的影响。方法 以质粒PJFH1为模板,通过PCR反应扩增4目的基因片段,采用同源重组法与PCMV-tag2b载体相连,转化大肠杆菌感受态DH5α,筛选正确的克隆。以脂质体为介导转染U2OS细胞,通过Western blot检测NS4B蛋白的表达,免疫荧光检测细胞的凋亡。结果 构建pCMV-tag2b-NS4B重组质粒,经测序其与NCBI公布的序列完全一致,并可在U2OS细胞中表达,DAPI检测显示细胞凋亡率为（17.25±2.95）%,对照组凋亡率为（6.53±2.36）%。结论 成功构建NS4B的真核表达载体,并可在U2OS细胞中表达,诱导细胞凋亡。山西省自然科学基金（No：2014021037-9）; 山西省高等学校大学生创新创业训练项目（No：2014052）; 山西医科大学汾阳学院科技发展基金（No：2016B02

‘福芳’甜椒嫁接植株之田間生育情形

In autumn crop, the graft success rate of 'Lucky Fragrance' sweet pepper were 100％, except grafted on 'M210101', 'Passion' and 194 rootstocks were 61.1％, 77％ and 77％, respectively. Flowering of these combinations were early then grafted on 'Miles Flavor', 'Passion' and 'Born Fire' rootstocks. The early fruit yield and total yield of plants grafted on 'Passion' and 'Born Fire' rootstockswere increased. The yields of plants grafted on 'Miles Flavor' and 'M210101' rootstocks were decreased. In spring crop, the field survival rate of 'Lucky Fragrance' sweet pepper grafted on 'M210101' and 'Born Fire' rootstocks were 88.9％ and 94.4％, respectively. Plants grafted on 'Miles Flavor' , 'Passion' and 'Born Fire' rootstocks enhanced anthesis. The plant height, stem node and fresh weight were increased by grafted on 'Passion' and '194' rootstocks. The plants had early fruit yield, fruit number and total yield in the combination of grafted on 'Passion' rootstock, but they were decreased when plants grafted on '31346','M210101' rootstocks and control.秋作以'福芳'接穗嫁接於M210101之成活率為61.1%,'百香'及'生生4號'砧者為77%，其他砧木者為100%。始花期以'百里香'、'百香'及'艷茹'砧顯著較早，其他地上部植株性狀於各穗砧組合無顯著差異。嫁接苗單株果實初期產量及總產量，以'百香'及'艷茹'砧較高，'百里香'及M2l0l0l玷之產童為最低。'福芳'春作嫁接於M210101砧之成活率為79.3%，定植30天之田間存活率除'艷茹'砧之94.4%及M210101砧之88.9%，其他組合皆為100%。始花期以'百里香'、'百香''及'艷茹'砧 顯著較早，嫁接植林之株高、節數及地上部鮮乾重以'百香'及'生生4號'砧顯 著最佳，31346砧及共砧之生育較差。'百香'砧嫁接苗單株初期產量，總產量果數為最高，31346、M210101砧及'福芳'對照植株為顯著最低

枯草桿菌處理對’青皮’苦瓜種子發芽之影響

The research studied the effect of application method and three Bacillus subtilus solution brands on seed germination of ‘Ching Pi' bitter groumd. The germination of ‘Ching Pi' bitter gourd seeds soaked with 60℃ water for 10 minutes, then 25℃ water for 24 hiurs were 86.7%. Mildew appeared on 10% of the ‘Ching Pi' control seeds. Germination of ‘Ching Pi' bitter gourd seeds Jun'solutions inhibited Rhizoctonia solani mycelium growth. The antibiotic content of ‘Huo Di' Jun' was greater than that of ‘Huo Li Bao' and ‘Yong Xin'. Bacillus spore number and antibiotic content in seed coated of seeds primed with B. subitilus solution were greater than that of soaked seed, and antibiotic content increased with time.苦瓜種子種皮厚且硬，在發芽過程中常有發霉的現象，會造成種子發芽率不佳及不整齊等問題，因此利用三品牌枯草桿菌以浸泡、披覆及滲調處理種子以改善種子發霉的問題。’青皮’苦瓜種子經60℃水浴10分鐘再浸25℃自來水24小時，發芽率為86.7%，發霉率為10%。’青皮’苦瓜種子以披覆或滲調活地菌發芽率分別為76.4%及83.3%，發霉率均為0%，以披覆及滲調處理種皮表面的孢子數及抗生素含量較高，且抗生素含量隨時間增加而增加。苦瓜種子種皮厚且硬，在發芽過程中常有發霉的現象，會造成種子發芽率不佳及不整齊等問題，因此利用三品牌枯草桿菌以浸泡、披覆及滲調處理種子以改善種子發霉的問題。’青皮’苦瓜種子經60℃水浴10分鐘再浸25℃自來水24小時，發芽率為86.7%，發霉率為10%。’青皮’苦瓜種子以披覆或滲調活地菌發芽率分別為76.4%及83.3%，發霉率均為0%，以披覆及滲調處理種皮表面的孢子數及抗生素含量較高，且抗生素含量隨時間增加而增加