Isolation and Identification of Secondary Metabolites from Halophytic Helianthustuberosus Tuber-Derived Fungus

盐生植物菊芋(Helianthus tuberosus)是一种新型经济作物,特殊生境可能会使其内生真菌产生特殊的次生代谢产物,如结构新颖或活性显著的化合物.为研究这种耐盐植物内生真菌的次生代谢产物以及植物宿主和内生真菌之间可能存在的关系,本实验室从耐盐菊芋块茎中分离纯化出10株内生真菌,对这10株内生真菌进行抗卤虫活性筛选,初步筛选出1株抗卤虫活性良好的菌株Mucor sp.ht-7,规模发酵后通过提取分离技术得到7个化合物,用波谱手段鉴定了7个化合物的结构,对这株内生真菌和宿主次生代谢产物之间的关系进行分析探讨,并对化合物生物活性加以分析

Abundance and production of bacteria and their correlations with environmental factor

以2014年8月南海北部海水样品为研究对象,利用平板计数法和流式细胞仪计数法对南海北部表层和垂直海域可培养细菌和细菌总数分布状况进行研究,对细菌; 生产力进行测定,并结合环境因子进行相关性分析。结果表明:珠江口到南海北部海域,水平方向可培养细菌总数变化范围是3.70*10~2 ~; 1.42*10~3 CFU/mL,细菌总数变化范围是5.12 * 10~5 ~1.61 * 10~6; cells/mL,细菌生产力的变化范围是0.03 ~0.40 mg/m~3/h;垂直方向上可培养细菌变化范围是1.08 *10~3 ~9.00; *10~3 CFU/mL,细菌生产力变化范围是0.01 ~0.08; mg/m~3/h,其中表层海水中的细菌生产力明显高于底层。与环境因子相关性分析表明,水平方向上,影响南海北部表层海水细菌总数和细菌生产力的主要因; 素是温度、盐度、硝酸盐(NO_3-N)、硅酸盐(SiO_3-Si)、亚硝酸盐(NO_2-N)和磷酸盐(PO_4-P)(P<0.05);垂直方向上; ,影响南海北部可培养细菌总数的主要因素是NO_2-N(P<0.05),影响细菌生产力的主要影响因素是温度和盐度(P; <0.05)。可见,南海北部表层海水中细菌总数高于可培养细菌总数2; ~3个数量级,表明该海域表层海水存在大量不可培养细菌;细菌的生命活动在海水表层相较底层更为活跃。Distribution of abundance and production of bacteria and their; correlations with environmental factor were investigated, using plate; count method and flow cytometry, in the surface and vertical waters of; the northern south China sea in August 2014. The cultivable bacterial; abundance ranged from 3.70 * 10~2 CFU/mL to 1.42 * 10~3 CFU/ mL, the; total bacterial abundance ranged from 5.12 * 10~5 cells/mL to 1.61 *; 10~6 cells/mL and the bacterial productions varied from 0.03 mg/m~3/h to; 0.40 mg/m~3/h in horizontal distribution. In vertical distribution, the; cultivable bacterial abundance ranged from 1.08 * 10~3 CFU/mL to 9.00 *; 10~3 CFU/mL and the bacterial productions varied from 0.01 mg/m~3/h to; 0.08 mg/m~3/h, respectively. The correlation analysis results showed; that the environmental factors affecting the abundance of total bacteria; and bacterial productivity included temperature, salinity,; nitrate(NO_3-N),silicate (SiO_3-Si), nitrite (NO_2-N),and phosphate; (PO_4-P) (P<0.05). NO_2-N was the main influencing factor to cultivable; bacteria abundance (P < 0.05), while bacterial productivity was highly; correlated with temperature and salinity in vertical distribution of; northern south China sea (P < 0.05). The total bacterial abundance was 2; ~ 3 orders of magnitude higher than cultivable bacteria, suggesting that; there were many uncultured bacterium in surface water of northern south; China sea and bacterial activities in the surface maybe more active than; in the bottom.全球变化与海气交互作用; 国家重点研发计划全球变化及应对专项; 海洋公益性行业科研专

Diversity of CO_2 fixation gene in the surface waters of northern South China Sea in the Calvin cycle

为揭示南海北部表层海水中参与卡尔文循环的固碳基因多样性及其与环境因子的关系,本研究以卡尔文循环中的关键酶核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubis CO)的Ⅰ、Ⅱ型基因(cbbL、cbbM)作为分子标记,采用Illumina Miseq高通量测序技术对海水中的固碳基因多样性进行分析,并结合多元统计分析的方法,探讨了固碳基因多样性与环境因子的关系.结果显示,在南海北部表层海水中,含cbbL基因固碳基因主要归属于变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)和厚壁菌门(Firmicutes),其中,优势亚群分别是γ-变形菌亚门(45.3%)、蓝细菌(30.9%)和β-变形杆菌亚门(23.8%);而含cbbM基因的固碳菌群未检测到;近岸的A9站位与其他站位物种组成有一定的差异,异着色菌属(Allochromatium)、硫杆菌属(Thiobacillus)和硫单胞菌属(Thiohalomonas)为其特有菌属.相关性和冗余分析(RDA)结果显示,含cbbL基因的固碳基因丰度与水温、盐度呈显著负相关(p<0.01),与硝酸盐、亚硝酸盐、硅酸盐呈显著正相关(p<0.01).To elucidate the diversity of CO_2 fixation and its relationship with environmental factors in surface water of northern South China Sea,the coding gene form I( cbbL) and form II( cbbM)( that encodes Ribulose-1,5-biphosphate carboxylase/oxygenase( Rubis CO) were chosen as biomarkers in Calvin-Benson-Bassham cycle( a common carbon fixation pathway for chemolithoautotrophic microorganisms) to analyze the carbon sequestration gene diversity. The Illumina Miseq sequencing method and multivariate statistical analysis were employed. The cbbM gene was not detected in any of samples,while the cbbL gene was found in all samples. The cbbL-containing genetic communities were dominated by Proteobacteria,Cyanobacteria and Firmicutes,in which γ-Proteobacteria( 45. 3%),Cyanobacteria( 30. 9%),β-Proteobacteria( 23. 8%) were predominant subpopulation. There were differences between the nearshore station of A9 with other stations,in which Allochromatiu,Thiobacillus and Thiohalomonas were unique species in A9 station.Relativity and redundancy analysis suggest that water temperature, salinity, nitrate, nitrite, silicate were highly statistically significant factors.Temperature and salinity were positively( p < 0. 01),while the other three factors negatively influenced CO_2 fixation gene of cbbL gene abundance( p <0. 01).全球变化与海气交互作用专项(No.GASI-03-01-02-05);; 海洋公益性行业科研专项(No.201305030,201405007)~

一种多氧生物碱类化合物及其制备和应用

本发明涉及抑藻剂、杀虫剂和抑菌剂领域，具体地说是一种海藻内生真菌来源的多氧生物碱类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将米曲霉(Aspergillus？oryzae)cf-2接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的多氧生物碱类化合物。本发明获得的多氧生物碱类化合物，经抑藻活性实验得出化合物半数抑制浓度为13.0微克/毫升，同时该化合物还具有杀虫和抑菌活性

Polyoxybiotic alkali compound as well as preparation method and application thereof

本发明涉及抑藻剂、杀虫剂和抑菌剂领域，具体地说是一种海藻内生真菌来源的多氧生物碱类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将米曲霉(Aspergillus oryzae)cf-2接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的多氧生物碱类化合物。本发明获得的多氧生物碱类化合物，经抑藻活性实验得出化合物半数抑制浓度为13.0微克/毫升，同时该化合物还具有杀虫和抑菌活性

Tetranuclear diterpenoids as well as preparation and application thereof

本发明涉及杀虫剂和抑菌剂领域，具体地说是一种海藻内生真菌来源的四环二萜类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将长梗木霉(Trichoderma longibrachiatum)cf-11接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的四环二萜类化合物。本发明获得的四环二萜类化合物，经杀虫活性实验得出化合物在100微克/毫升时对卤虫的致死率为82.6％，半数致死浓度为23.1微克/毫升，同时该化合物还具有抑菌活性

一种氯代生物碱类化合物及其制备和应用

本发明涉及抑藻剂、杀虫剂和抑菌剂领域，具体地说是一种海藻内生真菌来源的氯代生物碱类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将杂色曲霉(Aspergillus？versicolor)DL-29接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的氯代生物碱类化合物。本发明获得的氯代生物碱类化合物，经抑藻活性实验得出化合物半数抑制浓度为6.3微克/毫升，同时该化合物还具有杀虫和抑菌活性

一种四环二萜类化合物及其制备和应用

本发明涉及杀虫剂和抑菌剂领域，具体地说是一种海藻内生真菌来源的四环二萜类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将长梗木霉(Trichoderma？longibrachiatum)cf-11接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的四环二萜类化合物。本发明获得的四环二萜类化合物，经杀虫活性实验得出化合物在100微克/毫升时对卤虫的致死率为82.6%，半数致死浓度为23.1微克/毫升，同时该化合物还具有抑菌活性

一种降二萜类化合物及其制备和应用

本发明涉及杀虫剂、抑菌剂和肿瘤细胞抑制剂领域，具体地说是一种海藻内生真菌来源的降二萜类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将温特曲霉(Aspergillus?wentii)NA3接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的降二萜类化合物。本发明获得的降二萜类化合物，经杀虫活性实验得出化合物半数致死浓度为1.81微克/毫升，同时该化合物还具有抑菌和抑制肿瘤细胞的活性

Oxygen-rich disesquiterpenes compound, and preparation method and application thereof

本发明涉及杀虫剂领域，具体地说是一种海藻内生真菌来源的多氧二倍半萜类化合物及其制备和应用。具体结构式如(I)所示，其制备方法为将焦曲霉(Aspergillus ustus)cf-42接种于真菌液体培养基中发酵培养，发酵产物纯化后，即为式(I)所示的多氧二倍半萜类化合物。本发明获得的多氧二倍半萜类化合物，经杀虫活性实验得出化合物在100微克/毫升时对卤虫的致死率为87.1％，半数致死浓度为65.4微克/毫升