Expression of pin1 gene in lung cancer and its significance

背景与目的:Pin是人类高度保守的特异性磷酸化肽基脯氨酰顺及异构酶,它作用于脯氨酸所形成的肽键,并且仅使磷酸化pSer/Thr-Pro发生异构化,这一磷酸化后调控机制能诱导磷酸蛋白的构像变化,使其发挥功能。近来的研究显示这种新的调控机制在许多生理过程中起重要的调节作用,一旦失调便导致一系列人类疾病。如癌症患者体内Pin1表达异常增高,并调控多种癌基因的信号通路。本研究探讨pin1基因在非小细胞肺癌(NSCLC)中的表达及其意义。方法:利用免疫组织化学方法、定量PCR方法分别检测肺癌组织和正常肺组织中Pin1基因在翻译水平和mRNA水平上的表达差异。结果:在蛋白质水平上,Pin1的表达在正常/癌两种不同的肺组织中,表达量差异有显著性;而在mRNA水平上,Pin1的表达量在正常/癌两种不同的肺组织中,差异无显著性。结论:Pin1在NSCLC组织中的表达,可能是在翻译水平受到了调控;我们的研究进一步证实:Pin1在NSCLC组织中存在蛋白水平的过量表达,这对利用Pin1作为NSCLC组织检测标志物提供了理论依据。Background and purpose:Pin1 is a highly conservative enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Recent results indicate that such conformational changes following phosphorylation are a novel signaling mechanism pivotal in regulating many cellular functions. Overexpression of Pin1 is prevalently found in human cancers.We studied expression of pin1 in non-small cell lung cancer and its implication in clinical. Methods:We detected the differential expression level of Pin1 protein in lung cancer specimen and normal lung tissues by immunohistochemical staining. Real-time quantitative PCR was also applied to detect the mRNA differential expression level in lung cancer tissues and normal lung tissues. Results:Pin1 protein is overexpressed in lung cancer at the protein level. On the other hand, the expression level of Pin1 mRNA in lung cancer tissue has no significant change compared with normal tissue. Conclusion:It demonstrated that the Pin1 expression in lung cancer might be regulated by translation mechanism.Our data implicate that Pin1 may serve as a valuable molecular marker for human cancer

First record of genera Rutilaria and Trigonium(Bacillariophyta) and 11 diatom taxa(species and varieties) in South China Sea

Diatom taxa in samples collected from the uppermost 1-cm sediment of the South China Sea(SCS) during cruises in 2000,2001,and 2007 were investigated.Among them,two genera,Rutilaria and Trigonium,and 11 marine taxa(species and varieties) were described for the first time in China.The 11 taxa,Rutilaria radiata,Asterolampra grevillei,Biddulphia turrigera,Cocconeis cyclophora var.decora,Cocconeis ocellata,Dictyoneis marginata,Entogonia davyana,Tryblionella campechiana,Plagiogramma kinkeri,Plagiogramma nankoorense and Trigonium cf.contumax,were mainly extant species,although Rutilaria radiata and Entogonia davyana are fossils.Available data show that these species are mainly present in the eastern and western Pacific Ocean and the Indian Ocean.A large number of taxa newly recorded in this study were previously reported in the Philippines.The presence of these taxa in both locations is likely a result of seawater exchange in the SCS through the Bashi Channel.Taxonomic descriptions,habitats,and distributions of each..

Numerical simulation of gaseous detonation waves propagation and comparison with experimental results

本文应用基元反应模型和频散可控耗散格式(DCD)对氢氧爆轰波进行了二维数值模拟.氢氧混合物的化学反应模型考虑了8种组分20个反应方程式.在处理化学反应引起的刚性问题时采用了时间算子分裂的方法.本文首先对爆轰波数值结果和实验结果进行了对照验证,然后对爆轰波在楔面反射由马赫反射向规则反射转变的过程进行了数值分析,得到了反射转变临界角,并和实验结果及理论分析进行了比较,结果是令人满意的;本文还对爆轰波的多波结构进行了初步的数值分析

Time-frequency filtering method for detecting clicks of bottlenose dolphin (Tursiops truncates)

针对宽吻海豚Click信号检测提出了一种在信号时频图中基于Gabor滤波器的检测方法。该方法首先对声信号进行分段处理,计算每一段信号的时频图;然; 后设计Gabor滤波器,提取时频图中垂直方向的线条;对Gabor滤波处理后的时频图进行自适应阈值处理,提取时频图中能量较强的区域;最后通过连通域; 分析确定Click信号的位置。仿真合成不同信噪比的测试信号,本文算法在Click信号和背景噪声平均功率比为15; dB的情况下,Click信号的找全率达到了99%,错误率为0%;对实际采集的声信号进行Click信号检测,找全率为100%。本文方法预期为海豚观; 测和海豚生物学行为的研究提供一定的技术支持。A method based on Gabor filter is proposed for the detection of Pulses; of Bottlenose dolphin (Tursiops truncates). First, the acoustic signal; is divided into frames, and the spectrogram of each frame is calculated.; Then Gabor filter is designed to extract the vertical lines in the; spectrogram. Adaptive threshold is used to detect the regions where; pulses may be located in the spectrogram. Finally, the positions of the; pulses are determined by the connected component analysis. Simulating; test signal of different signal to noise ratio (SNR), the detection rate; is 99% and the error rate is 0% when SNR is 15 dB. Detecting pulses in; real world acoustic signal, the detection rate is 100%. The method is; expected to provide technical support for dolphin observation and the; study of dolphins' biological behavior

反应堆中微子实验的基线优化

介绍了在 χ2 假设检验中引入本底和系统误差的方法 ,并通过最大化混合角 θ13的测量敏感度对反应堆中微子实验的基线长度进行优化 .考察了对基线有影响的几个因素, 包括振荡效应, 统计误差, 系统误差, 本底等.尽管实验条件可能很不相同 , 最优基线仍可被确定在一个较小的范围内. We have applied the X2-minimization technique that takes backgrounds and systematic uncertainties into account to optimize the baseline of the reactor neutrino experiments designed for determining the mixing angle θ13 of neutrino. The effect of oscillation, systematic errors, statistic errors and backgrounds in the optimization are investigated. Although the experimental conditions may be very different, the optimal baseline is constrained in a narrow range

Additive manufacturing device and process for ceramic precursor material

本发明公开了一种用于陶瓷先驱体材料的增材制造装置和工艺，属于陶瓷材料的增材制造技术领域。该装置包括光路系统、支撑网板、升降机构、气氛循环及切换系统、成形腔和刮平机构；光路系统能够输出两种波长的激光光束，用于对工作区域进行扫描；采用光固化和原位烧结陶瓷化技术实现陶瓷类材料的直接成形，先平铺一层先驱体材料，采用光固化技术实现材料固化，再采用红外光烧结的方式将材料陶瓷化，如此依次逐层固化、逐层烧结最终实现复杂陶瓷结构的直接一体化成形。该成形工艺及装置可以实现先驱体材料陶瓷的一体化三维成形