Cloning and Construction Expressing Vector of Ovine Interleukin-2 Gene

从绵羊血中分离白细胞,提取绵羊白细胞RNA,利用RT-PCR、PCR扩增绵羊白细胞介素2(OvineIL-2)基因。将OvineIL-2PCR产物与TE载体定向连接,用SaLI和BamHI双酶切重组TE,利用低溶点胶回收500bp大小的片段;将其补平后与PPSD质粒连接,用EcoRI酶切鉴定,找出正向连接重组质粒。用BamHI酶切重组PPSD质粒,低熔点胶回收2500bp大小的片段;将其与经BamHI酶切去磷酸化的PME290表达质粒连接,筛选出阳性重组PME290,即为OvineIL-2基因表达载体。IL2 is known to be as an adjuvant in many areas. The ovine IL2 gene with synthesized recognition sequence at 5` and 3` end respectivecy was amplified and cloned from the peripheral blood of ovine. Ovine IL2 gene was amplified with ovine RNA as template by RTPCR and PCR. The amplified fragment was subjected to restriction digest and coined into the TE and PPSD vector. An expression plasmid was constructed by cloning the IL2 gene into PME290. The PME290SDIL2 was transformed into the host competent cell PAK/2pfs and the recombinant IL2 was expressed in the supernatant of the cultures of the transformant cell PAK/2pfs.国家科技部科研院所技术开发专项资金项目(国科发财字(1999)592号

Chemical constituents of bear bile

目的:系统研究中国传统名贵中药熊胆中的化学成分。方法:利用大孔树脂,SEPHAdEX lH-20,OdS及正相硅胶柱等色谱手段进行分离,通过多种波谱学数据分析进行单体化合物的结构鉴定。结果:从熊胆中分离得到9个单体化合物,经结构鉴定分别为4′,7-二羟基异黄酮(1),4′,7-二羟基-6-甲氧基异黄酮(2),4′,5,7-三羟基异黄酮(3),4′-甲氧基-7-羟基异黄酮(4),牛磺熊去氧胆酸(5),牛磺鹅去氧胆酸(6),熊去氧胆酸(7),鹅去氧胆酸(8),胆固醇(9)。结论:化合物1~4为首次从熊胆中分离,也是从熊科动物中首次分离得到。Objecctive: To study the chemical constituents of bear bile.Method: The compounds were isolated by repeated column HP20 macroporous adsorption resin,Sephadex LH-20,ODS and silica gel as packing materials.The structures were identified on the basis of extensive spectroscopic data analysis and by comparison of their spectral data reported.Result: Nine compounds were identified as 4′,7-dihydroxyisoflavone(1),4′,7-dihydroxy-6-methoxyisoflavone(2),4′,6,7-trihydroxyisoflavone(3),4′-methoxy-7-hydroxyisoflavone(4),tauroursodeoxycholic acid(5),taurochenodeoxycholic acid(6),ursodeoxycholic acid(7),chenodeoxycholic acid(8),cholesterol(9).Conclusion: Compounds 1-4 were separated from bear bile for the first time.厦门市科技计划项目(3502Z20083005

内蒙古呼伦贝尔市饮茶型氟铝联合中毒研究

目的探讨饮茶型氟中毒是否为砖茶型氟铝联合中毒。方法对饮茶型氟中毒病区进行饮水、砖茶、奶茶、主食、副食及牧民发、血、尿氟铝水平检测,对饮茶型氟中毒病区儿童和成人进行临床检查和拍摄 X 线片,同时对成人进行血液生物化学检查,以饮水型病区及非病区居民为对照组。结果呼伦贝尔市饮茶型病区饮水、主食、副食氟铝水平均在正常范围,砖茶氟铝水平分别为(541.3±48.8)、(4351±724)mg/kg,奶茶氟铝水平分别为(3.83±0.73)、(4.28±1.56)mg/L。饮水型病区饮水氟铝水平分别为(3.68±1.05)、(0.45±0.25)mg/L。饮茶型病区牧民氟总摄入量96.15%、铝总摄入量89.60%来源于砖茶。儿童氟斑牙检出率饮茶型病区为25.24%,饮水型病区为63.17%,饮茶型病区非常显著低于饮水型病区(P<0.01);成人氟中毒检出率饮茶型病区为42.61%,饮水型病区为28.83%,饮茶型病区显著高于饮水型病区(P<0.01)。结论呼伦贝尔市饮茶型氟中毒发病机理较为复杂,可能是砖茶型氟铝联合中毒