Cloning, Sequencing and Expression of a Chitinase Gene from Aeromonas caviae and Functional Disparity Between the Complete Chitinase and its 563-aa Truncant

豚鼠气单胞菌（Aeromonascaviae）CB101能产生多种分子量不同的几丁质酶。根据已知几丁质酶基因的DNA保守序列，利用人工合成的PCR引物和CB101总DNA，扩增出一个约2.6kb的片段并克隆到pBluescriptIIKS载体上。序列分析表明，这是目前已克隆的最大的微生物几丁质酶基因之一，命名为Chi1，编码865个氨基酸，蛋白质分子量为91.5kDa，从N端到C端依次包括N-末端信号肽序列，类PKD（Polycystickidneydisease,多囊肾）结构域，催化结构域，C端类REJ（ReceptorforEggJelly,卵胶膜受体）区域和两个几丁结合区，是目前已知的最...Aeromonas caviae can secrete several chintinases with different molecular weights. One chitinase gene chi1 has been cloned and sequenced. It encodes a protein of 865 amino acids with a signal peptide at the N-terminus, of which a polycystic-kidney-disease(PKD)-like domain, a triosephosphate-isomerase(TIM) catalytic region, a receptor-for-egg jelly(REJ)-like domain and two tandem chitin-binding-dom...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：19992601

Structures of the cloned chitinase and its truncant from A-caviae

Aeromonas caviae can secrete several chintinases with different molecular weights. One chitinase gene chi1 has been cloned and sequenced. It encodes a protein of 865 amino acids with a signal peptide at the N-terminus, a polycystic kidney disease(PKD)-like domain, a triosephosphate isomerase(TIM) catalytic region, a receptor for egg jelly(REJ)-like domain and two tandem chitin binding domains (ChBDs). The entire chitinase degrades colloid chitin both endolytically and exolytically into N-acetylglucosamine, chitobiose and chitotriose. When removing the 302 amino acids at C-teminus its activity remains but the degraded products are chitobiose, chitotriose and chitotetraose. The study shows that for the full-length chitinase, its substrate with the shortest length is chitotriose while in its truncated form, it is chitotetraose

Putative Exposed Aromatic and Hydroxyl Residues on the Surface of the N-Terminal Domains of Chi1 from Aeromonas caviae CB101 Are Essential for Chitin Binding and Hydrolysis

Chitinase Chi1 of Aeromonas caviae CB101 possesses chitin binding sites at both its N and C termini. Four putative exposed residues aligned in a line on the surface of the N-terminal domains of Chi1 were found to contribute to the enzyme-chitin binding and hydrolysis via site-directed mutagenesis. Also, it was found that Chi1 requires the cooperation of the N- and C-terminal domains to bind fully with crystalline and colloidal chitin

Quick identfication for pathogenic vibrio of aquacultivated creatures by UPPCR-SSCP

采用通用引物PCR配合SSCP分析即UPPCR SSCP技术 ,对弧菌科弧菌属中的副溶血弧菌、溶藻酸弧菌、费尼斯弧菌、鳗弧菌、哈维氏弧菌的 1 6S RNA基因进行检测。结果发现 ,UPPCR SSCP技术能较好地鉴别细菌的不同种 ,但对同种不同株细菌的区分较困难。认为PCR SSCP是将病原菌判定至种的有力工具。此研究对水产养殖病原菌的快速诊断与及时防治有重要意义。By universal primer PCR (UPPCR) amplification and SSCP, 16S DNA gene fragments of Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio furnissii, Vibrio anguillarum, Vibrio harveyi had been detected in this paper. It was found that different species of bacteria could been easily identified by this technique, but the same species could not. The findings suggest that UPPCR SSCP may be a potential tool for identifying the species of pathogenic bacteria. Therefore, this study will be of benefit in quick diagnosis and precaution in aquacultivated creatures.国家自然科学基金!(39770 5 85号 );; 国家海洋局第三海洋研究所海洋生物工程重点实验室开放课题;; IFS基金资助项 目!(A/ 2 338 2号

固相微萃取与色谱联用方法分析水中12种有机氯化合物

运用顶空固相微萃取与色谱联用方法(HS-SPME-GC)对水中的残留有机氯化合物进行了分析.对影响HS-SPME-GC分析灵敏度的各种实验因素如涂层种类、萃取温度、平衡时间、离子浓度等进行了讨论,并将该方法与固相萃取法(SPE)、液液萃取法(LLE)作了对比;同时考察了常见环境共存污染物直链烷基苯磺酸钠(LAS)对几种方法的影响

Nursing management for a patient with erysipelas infection leads to sepsis combined with multiple organ dysfunction syndromes (1例丹毒感染致脓毒症合并多功能脏器衰竭患者的护理体会)

This paper reviewed nursing management for a patient with erysipelas infection leads to sepsis combined with multiple organ dysfunction syndromes. A comprehensive treatment including anti-infective therapy, diuretic treatment, cardiac medication and circulation function treatment and related nursing management was carried out. Targeted nursing strategies including non-invasive ventilation nursing, medication care and fluid-management, fever nursing, catheter maintenance, skin care were carried out to ensure the therapeutic efficacy and symptom relieving. (本文总结1例丹毒感染致脓毒症合并多功能脏器衰竭患者的护理措施, 在给予抗感染、利尿、强心、维持循环功能治疗的同时还要加强护理, 从无创通气护理、用药监护及液体管理、发热护理、管路护理、皮肤护理等方面给予以针对性护理干预, 确保有效改善患者症状。

草炭绿化荒漠

1993-1996年与日本草炭研究会开始“草炭绿化荒漠”的研究工作,1997-2000年开始执行中日政府间JICA合作研究,1998年9月-2001年9月开始中国科学院重大国际合作特别支持项目。该项目以中国科学院阜康荒漠生态试验站为基地,利用草炭改良荒漠,寻求绿化荒漠的新方法、新技术,改善干旱区环境为目的。研究包括草炭的基本性质、土壤-植物系统与水份关系、草炭改土效果、草炭制剂的研究制、草炭利用新技术、草炭的土壤中分解速率和利用年限、草炭绿化荒漠机理等。研制的“草炭土壤调理剂”获发明专利,该制剂可为作物提供全方位的水份和养份供应,为有机肥工业化提供了良好前景;研究方法上采用了盆栽、小区和同位素..