Autophagy Inhibition by Chloroquine Sensitizes Cervical Cancer SiHa Cells to CPT Treatment

自噬诱导是肿瘤细胞对化疗药物抵抗性的原因之一,该研究探讨溶酶体抑制剂氯喹对喜树碱(camptothecin,CPT)诱导的宫颈癌细胞Si Ha死亡的增敏效果。CPT和/或氯喹处理宫颈癌Si Ha细胞,MTT法检测细胞增殖,DAPI和TUNEL染色观察细胞凋亡,Western blot和免疫荧光检测自噬及凋亡相关蛋白。结果发现,CPT处理后,Si Ha细胞MAP1LC3B荧光点和LC3II(microtubuleassociated protein light chain 3II)蛋白水平增加,p62荧光点和蛋白质水平则减少;而采用氯喹特异抑制自噬后,可明显提高CPT诱导的细胞凋亡、caspase-9的激活和PARP(poly ADP-ribose polymerase)的切割,而全长caspase-2水平显著下降。以上结果提示,氯喹可通过抑制细胞自噬而增强宫颈癌细胞株Si Ha对CPT诱导细胞凋亡的敏感性。The autophagy induction is one of the reasons for the resistance of tumor cells to chemotherapy drugs. In this study, the enhanced sensitivity of cervical cancer Si Ha cells to camptothecin(CPT)-induced cell death by chloroquine(a lysosome inhibitor) was investigated. The cell viability was detected by MTT assay, meanwhile, apoptosis was observed by DAPI and TUNEL, autophagy related proteins and apoptosis proteins were analyzed by immunofluorescence(IF) staining and Western blot in Si Ha cells after CPT treatment alone or combined with chloroquine. The results found that in Si Ha cells with CPT treatment the autophagy related protein LC3 foci and microtubule-associated protein light chain 3II(LC3II) protein level was increased, but p62 foci and protein level was decreased. When autophagy was inhibited by chloroquine, the CPT-induced apoptosis was obviously enhanced, and caspase-9 was activated and PARP was cleaved, but full length caspase-2 was decreased. Taken together, these results indicated that the inhibition of autophagy by chloroquine could sensitize cervical cancer Si Ha cells to CPT inducing cell apoptosis.山西省自然科学基金(批准号:2014021037-9);; 山西医科大学汾阳学院博士启动基金(批准号:1301);山西医科大学汾阳学院科研项目基金(批准号:1422)资助的课题~

Reclamation Dynamics Along the Mainland Coast of Shandong Province during 1974-2017

With the rapid socioeconomic development, human activities continue to expand from mainland toward the sea. In this context, reclamation has become an important way to solve the shortage of land resources in coastal areas. However, high-intensity and unreasonable reclamation has caused tremendous pressure on the eco-environment in the coastal zone. Timely and accurately monitoring regional reclamation is of great significance for the protection of coastal resources and the promotion of regional sustainable development. Shandong Province is a major marine province with a long history of reclamation, with prominent coastal eco-environment problems caused by reclamation. By summarizing the existing literature, we found that there are few studies focused on unraveling the human-environmetal laws of reclamation, especially in Shandong province. So we extracted the vector data of reclamation using remote sensing images, and analyzed the spatiotemporal dynamics along the mainland coast of Shandong Province from 1974 to 2017. The present study was based on RS/GIS technology, combined with a variety of data sources and aiming to inform reclamation management and related planning of the coastal zone in the study area. Results showed that: (1) The reclamation area of the mainland coast of Shandong Province had reached 4649.26 km~2 by 2017, presenting a sustained growth trend during the research period. The main type of utilization was sea-based reclamation. (2) Sea-based reclamation was mainly distributed in the Yellow River delta and the tidal flat areas of Laizhou Bay, while land-based reclamation was concentrated in the coastal areas of major ports and cities. The gravity center of reclamation moved from southeast to northwest during 1974-2017. (3) Frequent conversion from sea-based reclamation to land-based reclamation was prominent, and reclamation utilization types changed from being single to more diversified. The proportion of harbors, towns, industries, and other utilization types in reclamation development had increased rapidly, and the development of aquaculture and salt industry was no longer the main way. The reclamation utilization turned to be more comprehensive, diversified, and intensified. (4) Coastal reclamation in the whole province presented obvious multi-stage characteristics. Before 2000, reclamation in Shandong Province was mainly used to develop aquaculture and salt industry. While after 2000, especially from 2007 to 2017, the land-based reclamation area had increased rapidly, mainly used for port construction, urban and tourism infrastructure construction, and industrial development of coastal ports, due to the impact of the coastal development strategy, urbanization and industrialization. (5) The types of reclamation had been transformed between each other frequently. The new land in Shandong Province came not only from land reclamation to the sea, but also from the transformation of original reclamation types such as aquaculture ponds and salt fields.</p

Discussion on the development of china’s marine ranching

我国海洋牧场开发近几年取得了重要进展。本文在简要介绍海洋牧场概念、我国海洋牧场开发历史与现状基础上,指出当前我国海洋牧场开发建设中尚存在布局不合理、选址不科学、结构类型单一、运营模式粗放、管理困难等突出问题和挑战,并从体制转变、制度完善、政策引导和技术创新等方面提出了对策建议。China’s marine ranching has made a rapid development in recent years .On basis of a brief introduction to concept of marine ranching and the developing progress of china ’s marine ranching , this article considers that china’s marine ranching is confronted with a number of problems and chal‐lenges ,including unreasonable spatial arrangement ,unscientific location ,single type ,extensive oper‐ation mode ,and difficulties in management ,and discussed accordingly the countermeasures from as‐pects of system transform ,institution improvement ,policy design and technological innovation

Construction ofWetland Ecological Networks under Four Kinds of Resistance of Rivers in the Yellow River Delta and Their Comparison

Ecological network is an efficient tool to increase the landscape connectivity which plays important roles in maintain of ecosystem integrity and protection of key habitats. Resistance surface is one of the most important features of ecological network, determining the effectiveness of ecological network construction. The main objective of this paper was to analyze how water resistance surface can influence the connectivity of potential wetland ecological corridors in a typical coastal wetland of the Yellow River Delta. Four water resistance surface schemes were designed based on the values of water resistance cost, respectively: (1) a basic cost value, according to the current references; (2) half of the basic value, (3) the minimum cost value, and (4) decreasing of the cost interval. The cost distances and potential wetland ecological corridor for these four schemes were generated based on the least cost path method. The structure features and landscape connectivity characteristics of these networks of the four different schemes were evaluated using both network constructive indices and landscape connectivity indices. The results showed significant differences among the four potential wetland corridors. Both water cost and the cost interval had affected the ecological corridors significantly. When the value of water cost was decreased by half, the pattern of network 2 was similar with that of the network 1 which based on the referenced value. The number of nodes, the pattern of the potential corridors and their total length have changed slightly. However, when the cost value was the minimum, the number of nodes and the length of potential corridors had increased greatly. The potential corridors have overlapped with the real river networks, especially in the Yellow River tail channel and old channels. In addition, when the cost interval was decreased, the structure of network 4 became complex and the number of nodes, length of potential corridors have also increased. The assessment results of network connectivity also showed that the four designations were able to generate the closed networks and the landscape connectivity indices have increased due to the increasing of threshold values. In particular, when the threshold values were larger than 12 km, the landscape connectivity of network of the third scheme was the highest. The results also showed that the density of new nodes was more intensive in inland areas than that in other parts of the delta, indicating a more fragmented landscape pattern. Consequently, the connection between these wetland habitats should be strengthened. This research could give scientific suggestions for protection and management of the wetlands in the Yellow River Delta

Extraction and spatial analysis of impervious surfaces in the Bohai Bay region based on OLI imagery

不透水面的分布规模、空间格局和变化是影响区域生态系统和环境变化的重要指标。Landsat 8卫星的成功发射使得基于Landsat系列遥感影像的陆地生态环境监测及其科学研究得以延续。本研究以该卫星搭载的陆地成像仪(OLI)影像为遥感数据源,基于植被-不透水面-土壤(V-I-S)模型,采用最小噪声分离法(MNF)和支持向量机(SVM)相结合的方法实现地表信息遥感影像分类,并提取高反照和低反照覆盖初步提取不透水面,利用遥感指数去除水体、阴影等干扰物后,得到研究区不透水面的分布现状,在此基础上分析其空间格局。结果表明:相较于TM影像,OLI影像辐射分辨率提高为16bit,其波段范围普遍变窄,影像经大气..

海岸带生态系统科研价值评估——以环渤海区域为例

通过影子价格法、费用支出法、支付意愿法、机会成本法等生态系统评估方法对环渤海区域2009年海岸带生态系统科研的价值进行了估算。估算结果表明:2009年环渤海区域单位海岸线科研的实用价值为每年每公里5.478×106元,长效价值为每年每公里1.598×105元,剩余价值为每年每公里5.019×104元,总价值为每年每公里5.688×106元,其中,实用价值贡献率高达96.3%

Seepage analysis and control of asphalt core dam of Aqing hydropower station

水电站的渗流计算分析是一项复杂而在设计中又极其重要的工作,很难获得符合实际的渗流分析结果。结合阿青水电站的渗流控制分析,全面阐述了水电站渗流分析中熟悉工程概况和水文地质条件,明确分析任务、概化模型、剖分网格、选取材料参数,确定了计算方案和边界条件,整理分析计算结果,进行方案比较,分析了参数敏感性的方法和过程,认为应做好上述每一个环节,对全面把握工程的渗流性状,进行防渗排水体系的方案选择和优化的重要意义。分析表明,河床最大剖面的渗流量不一定比较低坝高剖面的渗流量大,狭窄河谷心墙堆石坝坝体和坝基总的渗流量,也可能比大坝最大剖面的渗流量与坝轴线长度的乘积大