Fabrication of Glass Microfluidic Chips By Electrochemical Micromachining

玻璃微流控芯片在许多领域已经得到较广泛的应用,但目前的加工需要繁琐的步骤及昂贵的设备进行图形转移及金属牺牲层开窗口。本文提出一种快速制作金属牺牲层图形窗口以用于玻璃微流控芯片加工的方法。以CO2激光直写加工PET膜模板,微细电解加工玻璃基片上的铬/金牺牲层快速获得窗口,湿法腐蚀及热键合制作玻璃微流控芯片。结果表明该法可在10秒内开窗口,电解加工过程使用的模板厚度、电解液组成及施加的压力与电压对窗口的质量都有显著影响。加工的微通道宽度为145μm,边缘整齐,宽度均匀,相对标准偏差为3.72%,深度25μm,底部平整度高,并成功用于氨基酸混合液的芯片毛细管电泳分离。同时使用该方法加工的金微电极阵列,电极宽度为100μm,最小间距可达100μm。Glass is a popular material for microfluidic chip,but its fabrication process includes pattern transferring and window-opening on sacrificial metal layer by photolithography is time consuming and high cost.Here a simple and rapid method for window-opening was developed.Cr/Au sacrificial film was developed by electrochemical micromachining using a PET through-mask ablated with CO2 laser system.Window-opening could be finished within 10 seconds,and its quality depends on the thickness of the PET mask,component of electrolyte,the pressure and electric voltage applied.The width and depth of the fabricated microchannel are 145μm and 25μm respectively,relative standard deviation is 3.72%.Electrophoretic separation of amino acid mixture was carried out successfully using the fabricated glass chip.And gold microelectrode array with 100μm width and 100μm interval was fabricated by this method too.国家自然科学基金资助项目(No.20675066);; 教育部留学回国人员科研启动基金资助项

Design and implementation of low voltage power distribution intelligente monitoring system based on isomeric architecture

为满足现代低压配电智能监控的需求,通过建立异构体系结构(B/S与三层C/S混合软件体系结构),有效地发挥了两种体系结构各自的优势,很好的将监控设备、企业内部信息资源、Internet有线网络资源与无线局域网资源等有机的集成在一起,同时通过WebService技术建立逻辑服务层,实现跨平台跨系统间的数据交互与整合。在整个系统实现过程中,依据软件工程的理论,使用面向对象的方法将复杂的系统需求逐一进行分解成数据监测、控制、实时报警模块,Web服务模块,数据信息管理模块和系统维护模块4大模块,并对每一个模块进行详细分析设计和应用实现,最终研制开发出低压配电智能监控系统。该系统具有很高的继承性、扩展性和重用性,能够满足工业低压配电智能系统的可扩充性、网络化、分布式系统的需求。In order to satisfy the modern low voltage power distribution intelligent monitoring requirements.this paper builds the isomeric architecture(Combine B/S and san layer C/S),which could use these two architectures’ advantages.It can integrate the monitoring equipment,enterprise internal information resources,network resource of internet and resource of LAN very well.It uses Web service technology and builds logic server,which can realize data exchange and integration in heterogeneous system platform.Based on the theories of the software engineering,the system decompose of monitoring,control and alarm real time model,Web service model,data management model and systems maintenance model.Low voltage power distribution intelligent monitoring system is designed by analyzing the above four models.This system has high inheritance,augment ability and reusablity;it meets the industry low voltage power distribution monitoring system’s requirements of augment ability,network and decentralization.福建省科技厅科技三项费用资助项目(2003H087

Electro-driving Permeation Performance of Nylon 6,6 Membrane

电驱动条件下膜分离性能的研究对膜在微流控芯片等微小器件中的应用具有重要的指导意义.研究了界面聚合的尼龙6,6膜的电驱动分离性能,并考察了电场强度、通电时间和温度等操作条件对SO42-和Cl-离子透过性能的影响.结果表明尼龙6,6膜具有较好的SO24-和Cl-离子透过性能,并且透过性能差异不大,离子透过百分比随电场强度和通电时间的增加而增加,而随温度的增加基本保持不变.而对fITC标记的甘氨酸和赖氨酸则能完全截留,截留分子量在500左右,具有部分纳滤膜性质.该膜可望应用于微型器件中氨基酸等有机与生物大分子的电驱动分离和浓缩.The study of separation properties of membrane under electro-driving condition is of an important guiding significance for the application of membrane in microdevices such as microfluidic chip.The electro-driving permeation performance of SO 24- and Cl- of Nylon 6,6 membrane which was prepared by interfacial polymerization was investigated, and effects of electric field, operation time and temperature on the SO 24- and Cl- permeation performance of Nylon 6,6 membrane were studied.Experimental results showed that the permeation percentage of SO 42- and Cl- are high and similar for nylon 6,6 membrane.Ion permeation percentage was increased with electric field strength and operation time, but not changed with temperature.However, FITC labeled glycine and lysine were blocked totally, the molecular weight cutoff is around 500.So in a broader sense, the synthesized nylon 6,6 membrane is one kind of nanofiltration membrane and could be used to separate and concentrate big organic or biological molecules in miniaturized devices.国家自然科学基金(No.20675066);国家基础科学人才培养基金(No.J0630429)资助项

木麻黄低效林不同发育阶段小枝养分和热值动态

对福建东南沿海防护林木麻黄低效林不同发育阶段小枝中养分(氮、磷)状况、内吸收率和热值的比较研究表明,木麻黄低效林小枝中氮含量分别高于正常林中相应发育阶段的小枝,而磷的含量在幼嫩小枝中低效林高于正常林,在成熟和衰老小枝中正常林高于低效林;低效林氮的内吸收效率稍低于正常林,而磷的内吸收效率则高于正常林;正常林和低效林小枝中的N:P比都高于16;低效林幼嫩和衰老小枝中的热值与正常林差别不显著,而低效林成熟小枝中的热值高于正常林。国家“十一五”科技支撑计划项目(2006BAD03A14-01);; 福建省重大科技专项(2006NZ0001-2);; 福建省森林培育与林产品加工利用重点实验室资助项

Nitrogen and phosphorus concentrations,N∶P ratio and resorption efficiency of leaves in different forest types

以福建东山沿海防护林中木麻黄、厚荚相思、刚果桉和湿地松4种主要树种为研究对象,探讨了纯林和混交林成熟叶与衰老叶中的养分状况、氮磷比、养分内吸收率以及它们之间的关系。结果表明:叶片中的养分浓度和内吸收率一般表现为纯林高于混交林、固氮树种高于非固氮树种。这是由于混交林和固氮树种改善了立地条件,林木能够从土壤中吸收较多的养分,从而降低了二者叶片中的养分内吸收率。各林分类型成熟叶片中的氮磷比都大于14,表明这些立地条件都存在不同程度的磷限制,导致大部分林分叶片磷内吸收率(rEP)大于氮内吸收率(rEn),因而衰老叶中的氮磷比大于成熟叶,但成熟叶和衰老叶氮磷比之间依然存在显著的正相关关系。不同林分类型成熟叶和衰老叶养分浓度、内吸收率和氮磷比在固氮树种和非固氮树种中存在互补性,表明固氮树种和非固氮树种在养分利用方面存在差异。不同树种的营养利用策略不同,在选择造林树种时,应优先考虑叶片养分含量低而内吸收率高的“营养保存型“树种。Nitrogen and phosphorus concentrations,N∶P ratio,nutrient resorption efficiency and their relationship in mature and senescent leaves in pure and mixed protection forest of Casuarina equisetifolia,Acacia crassicarpa,Eucalyptus ABL 12 W5 and Pinus elliottii were discussed in this paper.The results showed that nutrient concentration and resorption efficiency of leaves were higher in pure forest and N fixing species than in mixed forest and non-N-fixing species,respectively.This indicated that soil nutrient availability increased in mixed forest and N-fixing species,so the trees could absorb more nutrients from soil and depended less on nutrient resorption from senescent leaves.The N∶P ratios of mature leaves were all above 14,nitrogen was less limiting than phosphorus,and accordingly phosphorus resorption efficiency(RE_P)was higher than nitrogen resorption efficiency(RE_N).N∶P ratio was increased with leaf senescence.There was significant positive relationship between N∶P ratio in mature and in senescent leaves.Inter-and intraspecific correlations among nutrient concentrations,nutrient resorption and N∶P ratio of mature and senescent leaves in various forest types were different in N-fixing and non-N-fixing species.Due to the difference of nutrient use strategy for various life-forms,there was a significant correlation in N-fixing species,but no correlation in non-N-fixing species,and vice versa."Nutrient conservation" species,such as P.elliottii,with low leaf nutrient and high nutrient resorption efficiency should be prior selected for afforestation.“十一五”国家科技支撑计划项目(2006BAD03A14-01);福建省科技重大项目(2006NZ0001-2);国家林业局南方山地用材林培育重点实验室项

Sensitive Approaches for the Assay of the Global Protein Tyrosine Phosphorylation in Complex Samples Using a Mutated SH2 Domain

Sensitive Approaches for the Assay of the Global Protein Tyrosine Phosphorylation in Complex Samples Using a Mutated SH2 Domai

Highly efficient enrichment of phosphopeptides using porous zirconium-phytic acid hybrid

Highly efficient enrichment of phosphopeptides using porous zirconium-phytic acid hybri

基于NS2的TCP性能仿真分析

随着Internet的发展,网上传输的多媒体信息迅速增多,网络拥塞现象日益严重,Internet的服务质量QoS问题研究成为当前研究热点之一。实施拥塞控制应该是其他QoS机制正常工作的必要前提[1]。该文在介绍了免费网络仿真平台NS2构成及其原理、仿真平台搭建的基础上,对拥塞控制算法的性能进行了仿真分析,为今后进一步改进拥塞控制算法的TCP性能仿真研究奠定基础。国家自然科学基金(60371012);; 福建省科技重点项目,编号:2002Y02