Cytotoxic T-Lymphocyte Responses to Human Papillomavirus Type 16 E5 and E 7 Proteins and Hla-a*0201-Restricted T-Cell Peptides in Cervical Cancer Patients

Previously, we found that human papillomavirus type 16 (HPV- 16) E5 protein is a tumor rejection antigen and can induce cytotoxic T-lymphocyte (CTL) activity. Therefore, in this study, human leukocyte antigen A*0201 (HLA-A*0201)- restricted human CTL epitopes of HPV-16 E5 protein were identified using a bioinformatics approach, and the abilities of these predicted peptides to induce an immune response in HLA-A*0201 transgenic mice were confirmed by assaying E5-specific CTLs and in vitro-generated CTLs from normal peripheral blood T lymphocytes of HLA-A2-positive human donors. Second, the CTL responses to HLA-A*0201 CTL epitopes (E5 63-71 and E7 11-20) were examined in HPV-16- infected patients with HLA-A2. Third, the effect of HLA-A- type alleles on CTL activities in response to the entire E5 and E7 proteins was examined in cervical cancer patients. E5 and E7 peptides (but not the whole proteins) stimulated E5- and E7-specific CTL recall responses in HPV-16- and HLA-A2- positive cervical cancer patients, and HPV-16 E5 and E7 proteins stimulated naive T cells in HPV-16 -negative cervical cancer patients with HLA-A11 and -A24 haplotypes. In summary, this is the first demonstration that E5 63-71 is an HLA-A*0201 - restricted T-cell epitope of HPV-16 E5

日月潭創新導覽摺頁

[[abstract]]本次將傳統導覽手冊的內容加以改善，把QR code 加入導覽手冊中避免有厚厚一本的導覽手冊但實際上用到的只有地圖，不如把文字轉換成影片讓想了解的旅客，用自己的手機掃描一下便可簡單的獲取資訊。 透過文獻回顧我們將日月潭主要的六條步道挑選出來六條步道按照順序分別是，涵碧步道、水社親水步道、年梯步道、大竹湖步道、水蛙頭步道以及慈恩塔步道。將這六條步道的文化故事透過實際拍攝影片，來做說明。 再來將我們實際到日月潭拍攝的影片，放在youtube平台上，把網址轉成QR code 並貼在導覽手冊上，背後即是日月潭地圖，方便旅客尋找自己想要的資訊

日月潭創新導覽摺頁

[[abstract]]本次將傳統導覽手冊的內容加以改善，把QR code 加入導覽手冊中避免有厚厚一本的導覽手冊但實際上用到的只有地圖，不如把文字轉換成影片讓想了解的旅客，用自己的手機掃描一下便可簡單的獲取資訊。 透過文獻回顧我們將日月潭主要的六條步道挑選出來六條步道按照順序分別是，涵碧步道、水社親水步道、年梯步道、大竹湖步道、水蛙頭步道以及慈恩塔步道。將這六條步道的文化故事透過實際拍攝影片，來做說明。 再來將我們實際到日月潭拍攝的影片，放在youtube平台上，把網址轉成QR code 並貼在導覽手冊上，背後即是日月潭地圖，方便旅客尋找自己想要的資訊